LOCUS Exported 13503 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression vector containing PIK3C2A tagged with GFP. ACCESSION . VERSION . KEYWORDS pLEX303 GFP-PIK3C2A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13503) AUTHORS Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM TITLE The phospholipid PI(3,4)P2 is an apical identity determinant. JOURNAL Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. PUBMED 30487552 REFERENCE 2 (bases 1 to 13503) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13503 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 612..621 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 618..1334 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 618..1334 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(663..684) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(924..943) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1271..1292 /label=EGFP-C /note="EGFP, forward primer" CDS 6453..6470 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 6513..7101 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6566..6586) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6984..6995) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7173..7406 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7484..7605 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7521..7540) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7575..7594 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7645..7780 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7707..7726 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7800..7819) /label=T7 /note="T7 promoter, forward primer" promoter complement(7801..7819) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7829..7846) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7829..7845) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7838..7860) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7987..8442 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8074..8093) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8284..8305 /label=F1ori-F /note="F1 origin, forward primer" promoter 8468..8572 /gene="bla" /label=AmpR promoter CDS 8573..9433 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8791..8810) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9604..10192 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10093..10112 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10346..10363 /label=L4440 /note="L4440 vector, forward primer" protein_bind 10480..10501 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10516..10546 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10554..10570 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10559..10581 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10578..10594 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10578..10594 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10613..10633 /label=T3 /note="T3 promoter, forward primer" promoter 10615..10633 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 10661..10887 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 10888..11068 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 11115..11240 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11733..11966 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12151..12195 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 12370..12870 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 12789..12807 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 12882..13277 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 13337..13454 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agccctgcag ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 661 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 721 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 781 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 841 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 901 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 961 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 1021 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 1081 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 1141 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 1201 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 1261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 1321 acgagctgta caagtactca gatctcgagc tccaccgcgg tggcggccgc tctagaacta 1381 gtatggctca gatatttagc aacagcggat ttaaagaatg tccatcttca catccggaac 1441 caacaagagc aaaagatgtg gacaaagaag aagcattaca gatggaagca gaggctttag 1501 caaaactgca aaaggataga caagtgactg acaatcagag aggctttgag ttgtcaagca 1561 gcaccagaaa aaaagcacag gtttataaca agcaggatta tgatctcatg gtgtttcctg 1621 aatcagattc ccaaaaaaga gcattagata ttgatgtaga aaagctcacc caagctgaac 1681 ttgagaaact attgctggat gacagtttcg agactaaaaa aacacctgta ttaccagtta 1741 ctcctattct gagcccttcc ttttcagcac agctctattt tagacctact attcagagag 1801 gacagtggcc acctggatta cctgggcctt ccacttatgc tttaccttct atttatcctt 1861 ctacttacag taaacaggct gcattccaaa atggcttcaa tccaagaatg cccacttttc 1921 catctacaga acctatatat ttaagtcttc cgggacaatc tccatatttc tcatatcctt 1981 tgacacctgc cacacccttt catccacaag gaagcttacc tatctatcgt ccagtagtca 2041 gtactgacat ggcaaaacta tttgacaaaa tagctagtac atcagaattt ttaaaaaatg 2101 ggaaagcaag gactgatttg gagataacag attcaaaagt cagcaatcta caggtatctc 2161 caaagtctga ggatatcagt aaatttgact ggttagactt ggatcctcta agtaagccta 2221 aggtggataa tgtggaggta ttagaccatg aggaagagaa aaatgtttca agtttgctag 2281 caaaggatcc ttgggatgct gttcttcttg aagagagatc gacagcaaat tgtcatcttg 2341 aaagaaaggt gaatggaaaa tccctttctg tggcaactgt tacaagaagc cagtctttaa 2401 atattcgaac aactcagctt gcaaaagccc agggccatat atctcagaaa gacccaaatg 2461 ggaccagtag tttgccaact ggaagttctc ttcttcaaga agttgaagta cagaatgagg 2521 agatggcagc tttttgtcga tccattacaa aattgaagac caaatttcca tataccaatc 2581 accgcacaaa cccaggctat ttgttaagtc cagtcacagc gcaaagaaac atatgcggag 2641 aaaatgctag tgtgaaggtc tccattgaca ttgaaggatt tcagctacca gttactttta 2701 cgtgtgatgt gagttctact gtagaaatca ttataatgca agccctttgc tgggtacatg 2761 atgacttgaa tcaagtagat gttggcagct atgttctaaa agtttgtggt caagaggaag 2821 tgctgcagaa taatcattgc cttggaagtc atgagcatat tcaaaactgt cgaaaatggg 2881 acacagaaat tagactacaa ctcttgacct tcagtgcaat gtgtcaaaat ctggcccgaa 2941 cagcagaaga tgatgaaaca cccgtggatt taaacaaaca cctgtatcaa atagaaaaac 3001 cttgcaaaga agccatgacg agacaccctg ttgaagaact cttagattct tatcacaacc 3061 aagtagaact ggctcttcaa attgaaaacc aacaccgagc agtagatcaa gtaattaaag 3121 ctgtaagaaa aatctgtagt gctttagatg gtgtcgagac tcttgccatt acagaatcag 3181 taaagaagct aaagagagca gttaatcttc caaggagtaa aactgctgat gtgacttctt 3241 tgtttggagg agaagacact agcaggagtt caactagggg ctcacttaat cctgaaaatc 3301 ctgttcaagt aagcataaac caattaactg cagcaattta tgatcttctc agactccatg 3361 caaattctgg taggagtcct acagactgtg cccaaagtag caagagtgtc aaggaagcat 3421 ggactacaac agagcagctc cagtttacta tttttgctgc tcatggaatt tcaagtaatt 3481 gggtatcaaa ttatgaaaaa tactacttga tatgttcact gtctcacaat ggaaaggatc 3541 tttttaaacc tattcaatca aagaaggttg gcacttacaa gaatttcttc tatcttatta 3601 aatgggatga actaatcatt tttcctatcc agatatcaca attgccatta gaatcagttc 3661 ttcaccttac tctttttgga attttaaatc agagcagtgg aagttcccct gattctaata 3721 agcagagaaa gggaccagaa gctttgggca aagtttcttt acctcttttt gactttaaac 3781 ggtttttaac atgtggaact aaacttctat atctttggac ttcatcacat acaaattctg 3841 ttcctggaac agttaccaaa aaaggatatg tcatggaaag aatagtgcta caggttgatt 3901 ttccttctcc tgcatttgat attatttata caactcctca agttgacaga agcattatac 3961 agcaacataa cttagaaaca ctagagaatg atataaaagg gaaacttctt gatattcttc 4021 ataaagactc atcacttgga ctttctaaag aagataaagc ttttttatgg gagaaacgtt 4081 attattgctt caaacaccca aattgtcttc ctaaaatatt agcaagcgcc ccaaactgga 4141 aatgggttaa tcttgccaaa acttactcat tgcttcacca gtggcctgca ttgtacccac 4201 taattgcatt ggaacttctt gattcaaaat ttgctgatca ggaagtaaga tccctagctg 4261 tgacctggat tgaggccatt agtgatgatg agctaacaga tcttcttcca cagtttgtac 4321 aagctttgaa atatgaaatt tacttgaata gttcattagt gcaattcctt ttgtccaggg 4381 cattgggaaa tatccagata gcacacaatt tatattggct tctcaaagat gccctgcatg 4441 atgtacagtt tagtacccga tacgaacatg ttttgggtgc tctcctgtca gtaggaggaa 4501 aacgacttag agaagaactt ctaaaacaga cgaaacttgt acagctttta ggaggagtag 4561 cagaaaaagt aaggcaggct agtggatcag ccagacaggt tgttctccaa agaagtatgg 4621 aacgagtaca gtcctttttt cagaaaaata aatgccgtct ccctctcaag ccaagtctag 4681 tggcaaaaga attaaatatt aagtcgtgtt ccttcttcag ttctaatgct gtccccctaa 4741 aagtcacaat ggtgaatgct gaccctatgg gagaagaaat taatgtcatg tttaaggttg 4801 gtgaagatct tcggcaagat atgttagctt tacagatgat aaagattatg gataagatct 4861 ggcttaaaga aggactagat ctgaggatgg taattttcaa atgtctctca actggcagag 4921 atcgaggcat ggtggagctg gttcctgctt ccgataccct caggaaaatc caagtggaat 4981 atggtgtgac aggatccttt aaagataaac cacttgcaga gtggctaagg aaatacaatc 5041 cctctgaaga agaatatgaa aaggcttcag agaactttat ctattcctgt gctggatgct 5101 gtgtagccac ctatgtttta ggcatctgtg atcgacacaa tgacaatata atgcttcgaa 5161 gcacgggaca catgtttcac attgactttg gaaagttttt gggacatgca cagatgtttg 5221 gcagcttcaa aagggatcgg gctccttttg tgctgacctc tgatatggca tatgtcatta 5281 atgggggtga aaagcccacc attcgttttc agttgtttgt ggacctctgc tgtcaggcct 5341 acaacttgat aagaaagcag acaaaccttt ttcttaacct cctttcactg atgattcctt 5401 cagggttacc agaacttaca agtattcaag atttgaaata cgttagagat gcacttcaac 5461 cccaaactac agacgcagaa gctacaattt tctttactag gcttattgaa tcaagtttgg 5521 gaagcattgc cacaaagttt aacttcttca ttcacaacct tgctcagctt cgtttttctg 5581 gtcttccttc taatgatgag cccatccttt cattttcacc taaaacatac tcctttagac 5641 aagatggtcg aatcaaggaa gtctctgttt ttacatatca taagaaatac aacccagata 5701 aacattatat ttatgtagtc cgaattttga gggaaggaca gattgaacca tcatttgtct 5761 tccgaacatt tgacgaattt caggaacttc acaataagct cagtattatt tttccacttt 5821 ggaagttacc aggctttcct aataggatgg ttctaggaag aacacacata aaagatgtag 5881 cagccaaaag gaaaattgag ttaaacagtt acttacagag tttgatgaat gcttcaacgg 5941 atgtagcaga gtgtgatctt gtttgtactt tcttccaccc tttacttcgt gatgagaaag 6001 ctgaagggat agctaggtct gcagatgcag gttccttcag tcctactcca ggccaaatag 6061 gaggagctgt gaaattatcc atctcttacc gaaatggtac tcttttcatc atggtgatgc 6121 atatcaaaga tcttgttact gaagatggag ctgacccaaa tccatatgtc aaaacatacc 6181 tacttccaga taaccacaaa acatccaaac gtaaaaccaa aatttcacga aaaacgagga 6241 atccgacatt caatgaaatg cttgtataca gtggatatag caaagaaacc ctaagacagc 6301 gagaacttca actaagtgta ctcagtgcag aatctctgcg ggagaatttt ttcttgggtg 6361 gagtaaccct gcctttgaaa gatttcaact tgagcaaaga gacggttaaa tggtatcagc 6421 tgactgcggc aacatacttg taattaatta accaccacca ccaccaccac taaggatccc 6481 gtacgtgatc accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg 6541 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 6601 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 6661 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 6721 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 6781 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 6841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 6901 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 6961 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 7021 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 7081 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga 7141 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 7201 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7381 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca 7441 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 7501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 7561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 7621 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 7681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 7741 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc 7801 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg 7861 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 7921 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 7981 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 8041 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 8101 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 8161 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 8221 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 8281 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 8341 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 8401 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 8461 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 8521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 8581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 8641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 8701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 8761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 8821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 8881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 8941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 9001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 9061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 9121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 9181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 9241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 9301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 9361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 9421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 9721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 10201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc 10381 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10441 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10501 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10561 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa 10621 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc 10681 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 10741 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa 10801 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta 10861 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg 10921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 10981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 11041 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag 11101 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 11161 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 11221 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 11281 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 11341 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 11401 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 11461 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 11521 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 11581 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 11641 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 11701 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 11761 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta 11821 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 11881 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 11941 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 12001 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 12061 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 12121 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 12181 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 12241 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 12301 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 12361 aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct 12421 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc 12481 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct 12541 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg 12601 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg 12661 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc 12721 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg 12781 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc 12841 gttgaccgaa tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc 12901 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg 12961 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca 13021 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg 13081 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct 13141 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca 13201 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg 13261 gttatgtgtg ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg 13321 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa 13381 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa 13441 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg 13501 agg //