LOCUS       Exported                5134 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPro18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Lee SK, Keasling JD
  TITLE     A propionate-inducible expression system for enteric bacteria.
  JOURNAL   Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
  PUBMED    16269719
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     41..60
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    1972..2028
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2061..2078)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2231..2317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2409..2436
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2455..2546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2547..3407
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2765..2784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3449..3904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3536..3555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3746..3767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4015..4603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4504..4523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4757..4774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4789..4929
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4855..4874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5015..5037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
       61 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      121 tctgctcatg tttgacagct tatcatcgat tcagcttttc agccgccgcc agaacgtcgt
      181 ccggctgatg cctaaataat tcgccgctgc tgttttatcg ccattaaatt tctccagtgc
      241 ctgttgtggt gtcagtaagc gtggagcggg agttttcgcc gactcgcgcg ccagttccgg
      301 cagtagcagc tgcaaaaatt gcggcgttaa atccggcgtc ggttccacac ttaaaaatag
      361 cgccagtcgc tccatcatat tgcgcagttc acgaatattg cccggccagt cgtagtgcac
      421 cagcacggtt tcgcttgcct gtaatccctg gcgtaatgcg gcagaaaacg gggtggagag
      481 cgccgccaga gacactttca aaaagctttc cgccagtggc agaatatccg ccacccgctc
      541 gcgcagtggt ggcaattgca gacgcaaaat actcagccga taaaacaggt cacggcgaaa
      601 ctgcccttgc cgcatatctt cttccagatt gcagtgagtg gcgctaatga cccgcacatc
      661 caccggaaca ggctgatgcc cgccgacgcg ggtgacctct ttttcttcca gcacccgcag
      721 cagccgggtc tgcaacggca gcggcatttc gccaatctca tcgagaaaca gcgtacctcc
      781 gtgggcgatt tcaaacagcc cggcgcgacc gccgcgtcgc gagccggtaa acgccccttc
      841 ctcatagcca aacagttctg cttccagcag cgattcggca atcgccccgc agttgactgc
      901 aacaaacgga tgcgactttt tgccctgtcg cgcatcgtgg cgggcaaaat attcccgatg
      961 aatcgcctgg gccgccagct ctttgcccgt ccccgtttcc ccctcaatca acaccgctgc
     1021 actggagcgg gcatacagca aaatagtctg ccgtacttgt tccatctgtg gtgattgacc
     1081 gagcatatcg cccagcacgt aacgagttct cagggcgttg cgggtggcat cgtgagtgtt
     1141 atggcgtaac gacatgcgcg tcatatccag cgcatcgctg aacgcctggc gcacggtggc
     1201 ggcggaatag ataaaaattc cggtcattcc ggcttcttct gccagatcgg taatcagccc
     1261 cgcgccgacc accgcttcgg tgccgttagc ttttagctcg ttaatctgcc cgcgtgcgtc
     1321 ttcttcggta atgtagctac gttgatcgag gcgcaaatta aaggtttttt gaaacgccac
     1381 cagtgccgga atagtttcct gataagtgac aacgccgata gaagaggtga gttttccggc
     1441 ttttgccagt gcctgtaaca catcgtagcc gctcggttta atcaaaataa ctggcactga
     1501 caggcggctt ttcaggtacg cgccgttaga tccagcggcg atgatggcgt cacagcgttc
     1561 gtttgccagt ttcttgtgga tgtaggtcac cgctttttca aagccaagct ggataggggt
     1621 aatgttcgcc aggtgatcaa actcgaggct gatatcgcga aacagctcga acaggcgcgt
     1681 tacagatacc gtccagataa ccggtttgtc gtcattaagc cgtggtggat gtgccatagc
     1741 gcaccgcaaa gttaagaaac cgaatattgg gtttagtctt gtttcataat tgttgcaatg
     1801 aaacgcggtg aaacattgcc tgaaacgtta actgaaacgc atatttgcgg attagttcat
     1861 gactttatct ctaacaaatt gaaattaaac atttaatttt attaaggcaa ttgtggcaca
     1921 ccccttgctt tgtctttatc aacgcaaata acaagttgat aacaagctag cgaattcgag
     1981 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc
     2041 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     2101 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     2161 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     2221 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2281 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     2341 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     2401 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
     2461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2761 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3061 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     3121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3421 tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt aagcgcggcg
     3481 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3541 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3601 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3661 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3721 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3781 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3841 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3901 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4141 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4681 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4741 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4801 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4861 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     4921 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4981 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5041 tcaccgtcat caccgaaacg cgcgaggcag aaggagatgg cgcccaacag tcccccggcc
     5101 acggggcctg ccaccatacc cacgccgaaa caag
//
