LOCUS       Exported                8473 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers 
            Puromycin resistance. Inducible in T-REx cells..
ACCESSION   .
VERSION     .
KEYWORDS    pICE-EGFP-FLAG-PSMD2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  TITLE     Plasmids related to the study of the stereospecific bioactivation of
            lipidic alkynylcarbinols into reactive cytotoxic species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    1..225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      271..699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(358..377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     568..589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(708..728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        713..1042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      893..1028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     955..974
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1094..1693
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1094..1113)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1590..1610
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1823..1944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1860..1879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1914..1933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1994..2010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1994..2010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2007..2029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2018..2034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2042..2072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2225..2242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2396..2984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2476..2495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3155..4015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3778..3797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4016..4120)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4192..4571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4572..4775
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4726..4746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4777..4795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4798..4816
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4826..4850
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4931..4940
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4937..5653
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4937..5653
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4982..5003)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5243..5262)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5590..5611
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5669..5692
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8468..12)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
       61 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      661 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
      721 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      781 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      841 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      961 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     1021 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     1081 tcagcacgtg accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1141 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1201 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1261 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1321 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1381 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     1441 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1501 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1561 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1621 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1681 gcccggtgcc tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     1741 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1801 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     1861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1921 gtccaaactc atcaatgtat cttatcatgt ctggctagcc gtcgacctct agctagagct
     1981 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2041 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2101 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2161 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2821 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3001 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3061 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4141 cacctgacgt cgacggatcg ggagatcctg tacgggccag atatccgcgt tgacattgat
     4201 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4261 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4321 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4381 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4441 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4501 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4561 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4621 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4681 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4741 ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc
     4801 ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac
     4861 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggcaag
     4921 cttaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5101 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5521 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5641 cgagctgtac aagtccggac tcagatctga ttacaaggat gacgacgata agctcgagat
     5701 ggaggaggga ggccgggaca aggcgccggt gcagccccag cagtctccag cggcggcccc
     5761 cggcggcacg gacgagaagc cgagcggcaa ggagcggcgg gatgccgggg acaaggacaa
     5821 agaacaggag ctgtctgaag aggataaaca gcttcaagat gaactggaga tgctcgtgga
     5881 acgactaggg gagaaggata catccctgta tcgaccagcg ctggaggaat tgcgaaggca
     5941 gattcgttct tctacaactt ccatgacttc agtgcccaag cctctcaaat ttctgcgtcc
     6001 acactatggc aaactgaagg aaatctatga gaacatggcc cctggggaga ataagcgttt
     6061 tgctgctgac atcatctccg ttttggccat gaccatgagt ggggagcgtg agtgcctcaa
     6121 gtatcggcta gtgggctccc aggaggaatt ggcatcatgg ggtcatgagt atgtcaggca
     6181 tctggcagga gaagtggcta aggagtggca ggagctggat gacgcagaga aggtccagcg
     6241 ggagcctctg ctcactctgg tgaaggaaat cgtcccctat aacatggccc acaatgcaga
     6301 gcatgaggct tgcgacctgc ttatggaaat tgagcaggtg gacatgctgg agaaggacat
     6361 tgatgaaaat gcatatgcaa aggtctgcct ttatctcacc agttgtgtga attacgtgcc
     6421 tgagcctgag aactcagccc tactgcgttg tgccctgggt gtgttccgaa agtttagccg
     6481 cttccctgaa gctctgagat tggcattgat gctcaatgac atggagttgg tagaagacat
     6541 cttcacctcc tgcaaggatg tggtagtaca gaaacagatg gcattcatgc taggccggca
     6601 tggggtgttc ctggagctga gtgaagatgt cgaggagtat gaggacctga cagagatcat
     6661 gtccaatgta cagctcaaca gcaacttctt ggccttagct cgggagctgg acatcatgga
     6721 gcccaaggtg cctgatgaca tctacaaaac ccacctagag aacaacaggt ttgggggcag
     6781 tggctctcag gtggactctg cccgcatgaa cctggcctcc tcttttgtga atggctttgt
     6841 gaatgcagct tttggccaag acaagctgct aacagatgat ggcaacaaat ggctttacaa
     6901 gaacaaggac cacggaatgt tgagtgcagc tgcatctctt gggatgattc tgctgtggga
     6961 tgtggatggt ggcctcaccc agattgacaa gtacctgtac tcctctgagg actacattaa
     7021 gtcaggagct cttcttgcct gtggcatagt gaactctggg gtccggaatg agtgtgaccc
     7081 tgctctggca ctgctctcag actatgttct ccacaacagc aacaccatga gacttggttc
     7141 catctttggg ctaggcttgg cttatgctgg ctcaaatcgt gaagatgtcc taacactgct
     7201 gctgcctgtg atgggagatt caaagtccag catggaggtg gcaggtgtca cagctttagc
     7261 ctgtggaatg atagcagtag ggtcctgcaa tggagatgta acttccacta tccttcagac
     7321 catcatggag aagtcagaga ctgagctcaa ggatacttat gctcgttggc ttcctcttgg
     7381 actgggtctc aaccacctgg ggaagggtga ggccatcgag gcaatcctgg ctgcactgga
     7441 ggttgtgtca gagccattcc gcagttttgc caacacactg gtggatgtgt gtgcatatgc
     7501 aggctctggg aatgtgctga aggtgcagca gctgctccac atttgtagcg aacactttga
     7561 ctccaaagag aaggaggaag acaaagacaa gaaggaaaag aaagacaagg acaagaagga
     7621 agcccctgct gacatgggag cacatcaggg agtggctgtt ctggggattg cccttattgc
     7681 tatgggggag gagattggtg cagagatggc attacgaacc tttggccact tgctgagata
     7741 tggggagcct acactccgga gggctgtacc tttagcactg gccctcatct ctgtttcaaa
     7801 tccacgactc aacatcctgg ataccctaag caaattctct catgatgctg atccagaagt
     7861 ttcctataac tccatttttg ccatgggcat ggtgggcagt ggtaccaata atgcccgtct
     7921 ggctgcaatg ctgcgccagt tagctcaata tcatgccaag gacccaaaca acctcttcat
     7981 ggtgcgcttg gcacagggcc tgacacattt agggaagggc acccttaccc tctgccccta
     8041 ccacagcgac cggcagctta tgagccaggt ggccgtggct ggactgctca ctgtgcttgt
     8101 ctctttcctg gatgttcgaa acattattct aggcaaatca cactatgtat tgtatgggct
     8161 ggtggctgcc atgcagcccc gaatgctggt tacgtttgat gaggagctgc ggccattgcc
     8221 agtgtctgtc cgtgtgggcc aggcagtgga tgtggtgggc caggctggca agccgaagac
     8281 tatcacaggg ttccagactc atacaacccc agtgttgttg gcccacgggg aacgggcaga
     8341 attggccact gaggagtttc ttcctgttac ccccattctg gaaggttttg ttatccttcg
     8401 gaagaacccc aattatgatc tctaagcggc cgcacgcgtc tgcagtctag ataaacccgc
     8461 tgatcagcct cga
//
