LOCUS       Exported                8210 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human LHX6(LIM Homeobox 6) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLV.PGK.hLHX6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Giacomoni J, Bruzelius A, Stamouli CA, Rylander Ottosson D
  TITLE     Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells 
            into GABAergic Interneurons.
  JOURNAL   Cells. 2020 Nov 10;9(11). pii: cells9112451. doi: 
            10.3390/cells9112451.
  PUBMED    33182669
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1738..2248
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2167..2185
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(3480..3500)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3898..3909)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4058..4291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4363..4484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4400..4419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4454..4473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4524..4659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4586..4605
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4679..4698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4680..4698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4708..4725)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4708..4724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4717..4739)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4866..5321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4953..4972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5163..5184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5347..5451
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5452..6312
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5670..5689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6483..7071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6972..6991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7225..7242
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7359..7380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7395..7425
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7433..7449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7438..7460
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7457..7473
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7457..7473
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7492..7512
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7494..7512
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7613..7992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7993..8196
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8147..8167
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga attccacggg
     1741 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     1801 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     1861 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     1921 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     1981 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     2041 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     2101 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     2161 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     2221 tgaccgaatc accgacctct ctccccaggg ggatccaccg gtatgtactg gaagcatgag
     2281 aacgccgccc cggcgttgcc cgagggctgc cccgccaccg accaggtgat ggcccagcca
     2341 gggtccggct gcaaagcgac cacccgctgt cttgaaggga ccgcgccgcc cgccatggct
     2401 cagtctgacg ccgaggccct ggcaggagct ctggacaagg acgagggtca ggcctcccca
     2461 tgtacgccca gcacgccatc tgtctgctca ccgccctctg ccgcctcctc cgtgccgtct
     2521 gcaggcaaga acatctgctc cagctgcggc ctcgagatcc tggaccgata tctgctcaag
     2581 gtcaacaacc tcatctggca cgtgcggtgc ctcgagtgct ccgtgtgtcg cacgtcgctg
     2641 aggcagcaga acagctgcta catcaagaac aaggagatct tctgcaagat ggactacttc
     2701 agccgattcg ggaccaagtg tgcccggtgc ggccgacaga tctacgccag cgactgggtg
     2761 cggagagctc gcggcaacgc ctaccacctg gcctgcttcg cctgcttctc gtgcaagcgc
     2821 cagctgtcca ctggtgagga gttcggcctg gtcgaggaga aggtgctctg ccgcatccac
     2881 tacgacacca tgattgagaa cctcaagagg gccgccgaga acgggaacgg cctcacgttg
     2941 gagggggcag tgccctcgga acaggacagt caacccaagc cggccaagcg cgcgcggacg
     3001 tccttcaccg cggaacagct gcaggttatg caggcgcagt tcgcgcagga caacaacccc
     3061 gacgctcaga cgctgcagaa gctggcggac atgacgggcc tcagccggag agtcatccag
     3121 gtgtggtttc aaaactgccg ggcgcgtcat aaaaagcaca cgccgcaaca cccagtgccg
     3181 ccctcggggg cgcccccgtc ccgccttccc tccgccctgt ccgacgacat ccactacacc
     3241 ccgttcagca gccccgagcg ggcgcgcatg gtcaccctgc acggctacat tgagagtcag
     3301 gtacagtgcg ggcaggtgca ctgccggctg ccttacaccg caccccccgt ccacctcaaa
     3361 gccgatatgg atgggccgct ctccaaccgg ggtgagaagg tcatcctttt tcagtactaa
     3421 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3481 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3541 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3601 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3661 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3721 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3781 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     3841 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3901 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctaga gcctcttccg
     3961 cgtcttcgcc ttcccgggtc gagctcggta cctttaagac caatgactta caaggcagct
     4021 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4081 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4141 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4201 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4261 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4321 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     4381 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4441 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     4501 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4561 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4621 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     4681 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     4741 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4801 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4861 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4921 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5161 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5221 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5281 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     5341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6001 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6061 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6301 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6361 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6421 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6481 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6541 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6601 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6661 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6721 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6781 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6841 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6901 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6961 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7021 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7081 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7141 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7201 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7261 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7321 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7381 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7441 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     7501 cctcactaaa gggaacaaaa gctggagctg caagcttggc cattgcatac gttgtatcca
     7561 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
     7621 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7681 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     7741 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     7801 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     7861 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     7921 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7981 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     8041 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     8101 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     8161 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
//
