LOCUS Exported 9527 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable. ACCESSION . VERSION . KEYWORDS pKRG3-mU6-PUb-hSpCas9-pAc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9527) AUTHORS Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM TITLE A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. JOURNAL Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5. PUBMED 33436886 REFERENCE 2 (bases 1 to 9527) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9527 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 194..269 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" regulatory 1840..1849 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1852..1872 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1897..5997 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5998..6045 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 6061..6114 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6139..6738 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVWPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6139..6158) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6635..6655 /label=Puro-F /note="Puromycin resistance gene, forward primer" protein_bind complement(6743..6767) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" intron 6859..6924 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 7054..7074 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 7499..7633 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7536..7555) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7590..7609 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" CDS 7730..8590 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7948..7967) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8761..9349 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9250..9269 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9503..9520 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gggttggccg attcattaat gcaggcaact 61 cgtgaaaggt aggcggatca gcgaatgaaa tcgcccatcg agttgatacg tccatccatc 121 gctagaaccg cgttcgctgt agaagactat ataagagcag aggcaagagt agtgaaatgg 181 agacgacgtc tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc 241 aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt 301 aaaataaggc tagtccgttt ttagcgcttg cgccaattct gcagacaaat ggctatcttt 361 acatgtagct tgtgcattga atccaattat aatttgcctt ggcaccagct gagccagaca 421 agaaagaaag cttcccagaa gtatatcgat ttagaagggt tgacgtcact tgctgactgc 481 actaatacag caaatgatgc aattagaatg attcaagtga aattcccaaa ttactgattt 541 ttctctggat ttggttatca gattacattc gaagctaaga ttagctaccg aaattgtcga 601 tcaaatcagg aaatcctttc tctatcgaaa aaggcattcg cacatcttcc tctctatgcc 661 atatacacga agggtaggta cattgacgtc tttgccagaa gttgaactgc atcgttcaag 721 gtacagaatg aacgactaac agacacaagc acgttttgct gtccattcag acacagggat 781 ggtacccata ttcgatcgat atagagccat ccaaccgaac agaggtatat gtatgaatgt 841 attgctgaaa ttttctagaa gtacaaccac cactacgaca gtgtctataa aacgcccctg 901 caaaggcgaa accagctcaa tcgaatacgt ttcctagtgg agtgaacatt acgcggccca 961 agtaagcagt gccagtgcaa gtgaagtgaa gtctctagtg aaaaagagtg atccaattag 1021 ccagaggaga aaatttcaga gtgaacaaag ctttattcaa aggacaatta ctattaaatt 1081 ggtgaaagtg catttcggtg aagggaatct tctagtgaag gtaggtaaat taattgatga 1141 aattatagct atgagcgaaa actagtttgg tgaatgattc ctttgtcttt gaatgagcaa 1201 actattttcc aagatggcga ctattgagct ttgagtgatt agtgaaaatt tgcaacgcag 1261 tttcatcatc attgataaaa cccaattgtg attcacagcg ataatcatat ttcgttgaat 1321 catcgctact aattgaatta aatttctaga ataataagaa taacgtattt gctccgtcac 1381 atatctaaaa taaatatttt gatggtaatt acccattaag gtaatattaa cacatatcga 1441 gaaaaacctt gaggaaatcg tgaaaacttg aagatacgca atttcaaaac tacgtagttc 1501 aaagtcgaaa acaagttaat ttttcactta aaagtagggc gttgttgtga cgtcatcacc 1561 ttcaagtgta tatttttcac ttggcctgcg actgcaaacg cagacaaagc aaaacaagtt 1621 taaaacctgt cgtgtcgtgc tcgaagccaa aggcaatgaa tcaatatcaa atgagagttt 1681 gcatttcaca accaattact caagcgtttc ctcgtttctt tttctgctca acagagattt 1741 caacaccaag ctcgataagc ttgttcgaat ctcgagtgcg cgcttccgga ggtatacacc 1801 taggcggtac cactgcagtg aattcggagc tctaccggtg ccaccatggc cccaaagaag 1861 aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag catcggcctg 1921 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc 1981 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga 2041 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga 2101 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag 2161 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag 2221 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac 2281 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc 2341 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg 2401 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg 2461 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag 2521 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg 2581 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc 2641 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac 2701 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg 2761 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac 2821 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac 2881 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag 2941 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag 3001 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg 3061 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc 3121 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt 3181 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc 3241 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc 3301 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag 3361 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc 3421 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac 3481 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg 3541 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc 3601 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc 3661 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat 3721 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga 3781 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag 3841 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc 3901 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc 3961 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag 4021 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc 4081 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa 4141 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc 4201 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa 4261 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag 4321 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac 4381 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac 4441 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac 4501 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc 4561 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc 4621 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag 4681 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg 4741 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat 4801 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg 4861 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg 4921 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc 4981 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag 5041 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc 5101 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg 5161 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct 5221 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag 5281 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg 5341 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg 5401 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa 5461 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc 5521 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc 5581 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc 5641 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc 5701 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa 5761 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc 5821 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc 5881 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac 5941 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaaa 6001 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc aggcaagctt 6061 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctgctagc 6121 ggtagcggca gcggtagcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6181 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6241 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctg gcccgaccac 6541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6601 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 6661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6721 cgcaagcccg gtgcctaata ggacccagct ttcttgtaca aagtggtgac gtaagctagc 6781 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca 6841 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg 6901 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg 6961 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat 7021 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagttgac 7081 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga 7141 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa 7201 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata 7261 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa 7321 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt 7381 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 7441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7621 tcatgtctgg atcccgttta aactacgcgt aattcaaaca gggttctggc gtcgttctcg 7681 tactgttttc cccaggccag tgctttagcg ttattgaaaa aggaagagta tgagtattca 7741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 7801 cccagaaacg ctggtgaaag taaaggatgc tgaagatcag ttgggtgcac gagtgggtta 7861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 7921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 7981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 8041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 8101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 8161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 8221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 8281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 8341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 8401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 8461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 8521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 8581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 8641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 8701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 8761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 8821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 8881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 8941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 9001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 9061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 9121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 9181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 9241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 9301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 9361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 9421 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 9481 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gagaaga //