LOCUS Exported 904 bp ds-DNA linear SYN 28-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pET21a-Core Traptavidin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 904) AUTHORS Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M TITLE A streptavidin variant with slower biotin dissociation and increased mechanostability. JOURNAL Nat Methods. 2010 Apr 11. ():. PUBMED 20383133 REFERENCE 2 (bases 1 to 904) AUTHORS . TITLE Direct Submission JOURNAL Exported May 28, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..904 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 461..478 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(531..549) /label=T7 Term /note="T7 terminator, reverse primer" terminator 545..592 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(716..735) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 tgggaattcc ctctgaatat tttgtttact ttaagaagga gatatacata tggctgaagc 61 tggtatcacc ggcacctggt acaaccagct gggatccacc ttcatcgtta ccgctggtgc 121 tgacggtgct ctgaccggta cctacgaatc cgctgttggt aacgctgaag gcgattacgt 181 tctgaccggt cgttacgact ccgctccggc taccgacggt tccggaaccg ctctgggttg 241 gaccgttgct tggaaaaaca actaccgtaa cgctcactcc gctaccacct ggtctggcca 301 gtacgttggt ggtgctgaag ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac 361 cgaagctaac gcgtggaaat ccaccctggt tggtcacgac accttcacca aagttaaacc 421 gtccgctgct tcctaataaa agcttgcggc cgcactcgag caccaccacc accaccactg 481 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca 541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg 601 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 661 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 721 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 781 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 841 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 901 agtg //