LOCUS       Exported                2135 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Level 0 C-terminal tag  part for MoClo assembly, TTCG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    pEPQD0CM0028
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2135)
  TITLE     A modular toolkit for biofoundry-assisted expression of plant 
            proteins
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     159..177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      181..224
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             265..288
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      335..392
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(416..433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(587..1175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(667..686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1358..1452
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1473..2132)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2047..2066
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
       61 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
      121 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
      181 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcat
      241 tcgggcggga gcggtggaag cggctggagc catccgcagt tcgaaaaata agtcgaccgg
      301 ctgctaacaa agcccgagct tcgagaccct gcagtccggc aaaaaagggc aaggtgtcac
      361 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
      421 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      481 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      541 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1021 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1321 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     1381 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1441 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1501 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1561 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1621 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1681 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1741 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1801 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1861 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1921 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1981 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     2041 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     2101 acggtggtat atccagtgat ttttttctcc atttt
//
