LOCUS Exported 13443 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains. ACCESSION . VERSION . KEYWORDS pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13443) AUTHORS Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D TITLE Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator. JOURNAL Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138. Epub 2019 May 23. PUBMED 31034138 REFERENCE 2 (bases 1 to 13443) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13443 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 637..981 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 925..945 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 1058..1078 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1079..5179 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5198..5218 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5222..5242 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6281..6430 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 6443..6592 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" terminator 6619..6871 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(6906..6924) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 7108..7132 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 8433..9062 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 9491..10564 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 10630..10824 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(11060..11082) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 11168..11308 /label=bom /note="basis of mobility region from pBR322" primer_bind 11223..11242 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(11323..11340) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11494..12082) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11574..11593) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12170..12985) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 12893..12912 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" misc_feature 13418..13442 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat 61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga 121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc 181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag 241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat 361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga 481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa 541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa 601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat 661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt 721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca 781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga 841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga 901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag 961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca 1021 acaaacaaca ttacaattta ctattctagt cgaaatgccc aagaagaaga ggaaggtgga 1081 caagaagtac tccattgggc tcgctatcgg cacaaacagc gtcggctggg ccgtcattac 1141 ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag 1201 cataaagaag aacctcattg gcgccctcct gttcgactcc ggggaaacgg ccgaagccac 1261 gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct 1321 gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga 1381 ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat 1441 cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct 1501 tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat 1561 caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga 1621 caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa 1681 cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct 1741 cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat 1801 cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc 1861 caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat 1921 cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct 1981 gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat 2041 caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca 2101 actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata 2161 cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa 2221 aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca 2281 gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat 2341 cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa 2401 aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt 2461 cgcgtggatg actcgcaaat cagaagagac tatcactccc tggaacttcg aggaagtcgt 2521 ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct 2581 gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa 2641 cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg 2701 agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa 2761 acagctcaaa gaagattatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg 2821 agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa 2881 agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac 2941 ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct 3001 cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct 3061 gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt 3121 tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct 3181 cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca gtctccacga 3241 gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa 3301 ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga 3361 gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa 3421 gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga 3481 aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat 3541 gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg atgccatcgt 3601 gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa 3661 aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta 3721 ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa 3781 ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt 3841 tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa 3901 gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct 3961 ggtttcagat ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca 4021 ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc 4081 caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat 4141 cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat 4201 tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact 4261 tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac 4321 agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac 4381 cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg 4441 caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag 4501 tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga 4561 actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct 4621 cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc 4681 tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa 4741 aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta 4801 tgaaaagctc aaaggatctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca 4861 caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct 4921 cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat 4981 cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc 5041 agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt 5101 cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct 5161 ctctcagctc ggtggagaca gcagggctga ttcggacccg aagaaaaaga ggaaggtaga 5221 tcctaagaag aagagaaagg tactgttgga tccgggaaca cctatggacg ctgatttggt 5281 agcttcttct acagtagtgt gggaacaaga tgcagatcct tttgctggaa cagccgacga 5341 cttccccgcc ttcaatgaag aggagcttgc atggttgatg gagctgttac ctcaaggagg 5401 atcagggggt cttttagatc caggtactcc catggatgca gacctggtcg cttcaagtac 5461 agtcgtttgg gagcaggatg ctgatccatt cgcaggtaca gcagatgact tcccggcttt 5521 caatgaggag gaacttgctt ggctcatgga attgctcccg caggcaagag gtggatctgg 5581 tggtctgctc gacccaggaa cgccgatgga cgctgatctg gtagcatcta gtacagtctg 5641 ggagcaggac gctgacccat tcgctggtac ggctgacgat tttcctgcct ttaatgagga 5701 agagttagcc tggctgatgg agttattacc ccagggcggt agtggcggat tattggaccc 5761 tggtacgccg atggatgcag accttgtggc ttcatcaacg gtcgtatggg agcaggatgc 5821 cgatcctttc gcaggaaccg cagacgactt cccagccttt aacgaagaag agctcgcatg 5881 gcttatggaa cttttacctc aggcacgtgg aggaagtggt gggctcctgg atccaggcac 5941 tccgatggac gcagacttag tagctagttc caccgttgtc tgggagcagg atgcagatcc 6001 ttttgctggt accgcagacg acttccctgc tttcaatgaa gaagaattgg cctggcttat 6061 ggaattactt ccgcagggtg ggtctggtgg gctgctcgat cccggtacgc cgatggatgc 6121 tgacttggtt gctagcagta ctgtggtctg ggaacaggat gctgacccct tcgctgggac 6181 tgccgacgac ttcccagcct tcaacgaaga ggaactcgca tggttgatgg aactgcttcc 6241 tcaagcacgt ggggggtcag gcggaggcgg atctggcggt gacgcactgg acgactttga 6301 tctcgacatg cttgggtccg atgctctcga cgattttgac ctcgatatgc ttgggtctga 6361 cgcattagat gactttgatt tagacatgtt ggggtcagac gctttggacg atttcgactt 6421 agatatgttg gccagaggta gtgatgccct tgacgacttc gatttagata tgttgggctc 6481 tgacgccctc gatgacttcg atctggatat gctcggaagt gacgccttgg acgatttcga 6541 cttggacatg cttgggtcgg acgccctcga cgactttgat ctggatatgt tatgagcttg 6601 gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat 6661 cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta 6721 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg 6781 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta 6841 tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg 6901 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 6961 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 7021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg 7081 ggaccgactc gcgcttgaga cgaagcttga caggatatat tggcgggtaa acctaagaga 7141 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg 7201 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 7261 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa 7321 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 7381 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 7441 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 7501 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 7561 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 7621 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 7681 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 7741 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 7801 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 7861 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 7921 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 7981 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 8041 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 8101 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 8161 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 8221 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 8281 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 8341 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 8401 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 8461 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 8521 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 8581 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 8641 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 8701 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 8761 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 8821 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 8881 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 8941 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 9001 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 9061 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 9121 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 9181 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 9241 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 9301 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 9361 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 9421 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg 9481 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg 9541 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc 9601 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg 9661 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc 9721 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga 9781 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga 9841 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga 9901 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga 9961 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt 10021 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt 10081 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac 10141 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg 10201 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa 10261 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg 10321 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa 10381 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg 10441 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc 10501 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc 10561 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg 10621 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa 10681 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga 10741 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct 10801 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa 10861 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc 10921 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca 10981 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt 11041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc 11101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 11161 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 11221 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 11281 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 11341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 11401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 11461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 11521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 11581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 11641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 11701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 11761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 11821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 11881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 11941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 12001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 12061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 12121 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact 12181 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt 12241 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa 12301 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc 12361 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg 12421 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct 12481 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga 12541 gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc 12601 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata 12661 cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac 12721 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca 12781 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg 12841 catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag 12901 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag 12961 acgtttcccg ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag 13021 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt 13081 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg 13141 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc 13201 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca 13261 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa 13321 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg 13381 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa 13441 aca //