LOCUS Exported 3495 bp ds-DNA circular SYN 17-JAN-2024 DEFINITION Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments. ACCESSION . VERSION . KEYWORDS pRL-SV40P SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3495) AUTHORS Chen X, Prywes R TITLE Serum-induced expression of the cdc25A gene by relief of E2F-mediated repression. JOURNAL Mol Cell Biol. 1999 Jul . 19(7):4695-702. PUBMED 10373518 REFERENCE 2 (bases 1 to 3495) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2024 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3495 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 669..865 /label=SV40 promoter /note="SV40 early promoter" rep_origin 716..851 /label=SV40 ori /note="SV40 origin of replication" primer_bind 778..797 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 935..1067 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 1050..1069 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1112..1130 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1140..2075 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 2106..2227 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2117..2136) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2171..2190 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 2360..2464 /gene="bla" /label=AmpR promoter CDS 2465..3325 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2683..2702) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat 661 ctgcgatctg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 721 gcccctaact ccgcccagtt ccgcccattc tccgccccat cgctgactaa ttttttttat 781 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 841 ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt 901 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt 961 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata 1021 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca 1081 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagccacca 1141 tgacttcgaa agtttatgat ccagaacaaa ggaaacggat gataactggt ccgcagtggt 1201 gggccagatg taaacaaatg aatgttcttg attcatttat taattattat gattcagaaa 1261 aacatgcaga aaatgctgtt atttttttac atggtaacgc ggcctcttct tatttatggc 1321 gacatgttgt gccacatatt gagccagtag cgcggtgtat tataccagac cttattggta 1381 tgggcaaatc aggcaaatct ggtaatggtt cttataggtt acttgatcat tacaaatatc 1441 ttactgcatg gtttgaactt cttaatttac caaagaagat catttttgtc ggccatgatt 1501 ggggtgcttg tttggcattt cattatagct atgagcatca agataagatc aaagcaatag 1561 ttcacgctga aagtgtagta gatgtgattg aatcatggga tgaatggcct gatattgaag 1621 aagatattgc gttgatcaaa tctgaagaag gagaaaaaat ggttttggag aataacttct 1681 tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt agaaccagaa gaatttgcag 1741 catatcttga accattcaaa gagaaaggtg aagttcgtcg tccaacatta tcatggcctc 1801 gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt acaaattgtt aggaattata 1861 atgcttatct acgtgcaagt gatgatttac caaaaatgtt tattgaatcg gacccaggat 1921 tcttttccaa tgctattgtt gaaggtgcca agaagtttcc taatactgaa tttgtcaaag 1981 taaaaggtct tcatttttcg caagaagatg cacctgatga aatgggaaaa tatatcaaat 2041 cgttcgttga gcgagttctc aaaaatgaac aataattcta gagcggccgc ttcgagcaga 2101 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 2161 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 2221 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 2281 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg 2341 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 2401 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 2461 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 2521 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 2581 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 2641 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 2701 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 2761 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 2821 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 2881 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 2941 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 3001 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 3061 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 3121 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 3181 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 3241 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 3301 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 3361 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 3421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 3481 gatcaaagga tcttc //