LOCUS Exported 6609 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mammalian expression and localization. ACCESSION . VERSION . KEYWORDS Nsp3 -mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6609) AUTHORS Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, Mathur P, Schauer K, Goud b, Albanese V, Gautier R, Subra M, Kovacs D, Barelli H, Antonny B TITLE A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types JOURNAL bioRxiv 423586 REFERENCE 2 (bases 1 to 6609) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6609 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 5839..5891 /label=MCS /note="multiple cloning site" regulatory 5893..5902 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 5899..6609 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(6044..6062) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6312..6331 /label=mCherry-F /note="mCherry, forward primer" ORIGIN 1 atggccccta ccaaggtgac cttcggagac gacaccgtga tcgaggtgca gggttacaag 61 agcgtgaaca tcaccttcga gctggacgaa cgcatcgaca aggtgctgaa cgaaaagtgc 121 tccgcttaca ccgtggagct gggcaccgag gtgaacgaat tcgcctgcgt ggtggccgac 181 gctgtgatca agaccctgca gccagtgtcc gaactgctga cccctctggg tatcgacctg 241 gacgagtggt ctatggctac ctactacctg ttcgacgaga gcggcgaatt caagctggcc 301 tctcacatgt actgcagctt ctaccctccc gacgaagacg aggaagaggg tgactgcgaa 361 gaggaagagt tcgagccttc cactcagtac gagtacggca ccgaagacga ctaccagggc 421 aagcctctgg aattcggagc cacctctgcc gctctgcagc ctgaagagga acaggaggaa 481 gactggctgg acgacgactc ccagcagacc gtgggtcagc aggacggctc tgaagacaac 541 cagaccacca ccatccagac catcgtggag gtgcagcctc agctggagat ggaactgacc 601 ccagtggtgc agaccatcga ggtgaacagc ttctccggct acctgaagct gaccgacaac 661 gtgtacatca agaacgctga catcgtggag gaagccaaga aggtgaaacc aaccgtggtg 721 gtgaacgccg ctaacgtgta cctgaagcac ggcggaggtg tggctggagc tctgaacaag 781 gctaccaaca acgccatgca ggtggaatcc gacgactaca tcgctaccaa cggtcctctg 841 aaggtgggcg gatcctgcgt gctgtctggc cacaacctgg ccaagcactg cctgcacgtg 901 gtgggaccaa acgtgaacaa gggagaggac atccagctgc tgaagagcgc ctacgaaaac 961 ttcaaccagc acgaggtgct gctggctcca ctgctgtctg ccggaatctt cggtgctgat 1021 cccatccaca gcctgcgcgt gtgcgtggac accgtgagga ccaacgtgta cctggccgtg 1081 ttcgacaaga acctgtacga caagctggtg agctccttcc tggagatgaa gtccgaaaag 1141 caggtggagc agaagatcgc cgagatcccc aaggaggaag tgaagccttt catcaccgaa 1201 tctaagccca gcgtggagca gagaaagcag gacgacaaga agatcaaggc ctgcgtggag 1261 gaagtgacca ccaccctgga ggaaaccaag ttcctgaccg agaacctgct gctgtacatc 1321 gacatcaacg gcaacctgca ccctgactcc gctaccctgg tgtctgacat cgacatcacc 1381 ttcctgaaga aggacgcccc ctacatcgtg ggcgacgtgg tgcaggaagg agtgctgacc 1441 gctgtggtca tccctaccaa gaaggccggt ggcaccaccg agatgctggc caaggctctg 1501 cgtaaggtgc caaccgacaa ctacatcacc acctaccccg gtcagggcct gaacggttac 1561 accgtggagg aagctaagac cgtgctgaag aagtgcaagt ctgccttcta catcctgccc 1621 tccatcatct ctaacgagaa gcaggaaatc ctgggcaccg tgagctggaa cctgcgcgaa 1681 atgctggccc acgctgagga aaccaggaag ctgatgcccg tgtgcgtgga gaccaaggcc 1741 atcgtgagca ccatccagag aaagtacaag ggcatcaaga tccaggaggg tgtggtggac 1801 tacggcgctc gtttctactt ctacacctcc aagaccaccg tggcctctct gatcaacacc 1861 ctgaacgacc tgaacgagac cctggtgacc atgccactgg gatacgtgac ccacggtctg 1921 aacctggagg aagccgctag atacatgcgt tccctgaagg tgcctgctac cgtgagcgtg 1981 agcagccctg acgctgtgac cgcctacaac ggatacctga cctcctctag caagacccct 2041 gaggaacact tcatcgaaac catcagcctg gccggttcct acaaggactg gtcttacagc 2101 ggacagtcca cccagctggg catcgagttc ctgaagcgcg gagacaagag cgtgtactac 2161 acctccaacc caaccacctt ccacctggac ggtgaagtga tcaccttcga caacctgaag 2221 accctgctgt ctctgcgcga ggtgaggacc atcaaggtgt tcaccaccgt ggacaacatc 2281 aacctgcaca cccaggtggt ggacatgagc atgacctacg gacagcagtt cggtcctacc 2341 tacctggacg gagctgacgt gaccaagatc aagccacaca actcccacga gggcaagacc 2401 ttctacgtgc tgcctaacga tgacaccctg agagtggagg ctttcgaata ctaccacacc 2461 accgacccat ccttcctggg caggtacatg tctgccctga accacaccaa gaagtggaag 2521 tacccccagg tgaacggact gaccagcatc aagtgggccg acaacaactg ctacctggcc 2581 accgctctgc tgaccctgca gcagatcgaa ctgaagttca acccacccgc tctgcaggac 2641 gcttactaca gagcccgtgc tggcgaggcc gctaacttct gcgctctgat cctggcctac 2701 tgcaacaaga ccgtgggcga actgggagac gtgagagaga ccatgagcta cctgttccag 2761 cacgccaacc tggactcctg caagcgtgtg ctgaacgtgg tgtgcaagac ctgcggccag 2821 cagcagacca ccctgaaggg agtggaagct gtgatgtaca tgggtaccct gtcctacgag 2881 cagttcaaga agggcgtgca gatcccttgc acctgcggaa agcaggccac caagtacctg 2941 gtgcagcagg aaagcccatt cgtgatgatg tccgctcctc cagcccagta cgaactgaag 3001 cacggcacct tcacctgcgc ttccgagtac accggcaact accagtgcgg acactacaag 3061 cacatcacct ctaaggagac cctgtactgc atcgacggag ccctgctgac caagtcctct 3121 gaatacaagg gtcccatcac cgacgtgttc tacaaggaga acagctacac caccaccatc 3181 aagcccgtga cctacaagct ggacggcgtg gtgtgcaccg aaatcgaccc taagctggac 3241 aactactaca agaaggacaa ctcttacttc accgagcagc ctatcgacct ggtgccaaac 3301 cagccctacc ctaacgccag cttcgacaac ttcaagttcg tgtgcgacaa catcaagttc 3361 gctgacgacc tgaaccagct gaccggatac aagaagcctg cctctagaga gctgaaggtg 3421 accttcttcc cagacctgaa cggcgacgtg gtggctatcg actacaagca ctacacccca 3481 agcttcaaga agggagccaa gctgctgcac aagcccatcg tgtggcacgt gaacaacgct 3541 accaacaagg ccacctacaa gcctaacacc tggtgcatcc gttgcctgtg gtctaccaag 3601 ccagtggaaa cctccaactc tttcgacgtg ctgaagagcg aggacgctca gggcatggac 3661 aacctggcct gcgaagacct gaagcccgtg agcgaggaag tggtggagaa cccaaccatc 3721 cagaaggacg tgctggaatg caacgtgaag accaccgagg tggtgggaga catcatcctg 3781 aagccagcta acaacagcct gaagatcacc gaggaagtgg gtcacaccga cctgatggcc 3841 gcttacgtgg acaacagctc cctgaccatc aagaagccca acgagctgag cagggtgctg 3901 ggtctgaaga ccctggctac tcacggtctg gctgctgtga actctgtgcc atgggacacc 3961 atcgctaact acgccaagcc tttcctgaac aaggtggtgt ccaccaccac caacatcgtg 4021 accagatgtc tgaaccgcgt gtgcaccaac tacatgccct acttcttcac cctgctgctg 4081 cagctgtgca ccttcaccag aagcaccaac tcccgtatca aggctagcat gcctaccacc 4141 atcgccaaga acaccgtgaa gagcgtggga aagttctgcc tggaggcttc cttcaactac 4201 ctgaagtctc caaacttcag caagctgatc aacatcatca tctggttcct gctgctctcc 4261 gtgtgcctgg gttccctgat ctactctacc gccgctctgg gagtgctgat gtctaacctg 4321 ggtatgccta gctactgcac tggttacaga gagggatacc tgaacagcac caacgtgacc 4381 atcgccacct actgcaccgg cagcatccca tgctccgtgt gcctgtctgg actggacagc 4441 ctggacacct acccctccct ggaaaccatc cagatcacca tctctagctt caagtgggac 4501 ctgaccgcct tcggtctggt ggctgagtgg ttcctggcct acatcctgtt caccaggttc 4561 ttctacgtgc tgggcctggc cgctatcatg cagctgttct tctcctactt cgctgtgcac 4621 ttcatcagca actcctggct gatgtggctg atcatcaacc tggtgcagat ggctcccatc 4681 tctgccatgg tgcgcatgta catcttcttc gccagcttct actacgtgtg gaagtcctac 4741 gtgcacgtgg tggacggttg caactcctct acctgcatga tgtgctacaa gcgcaacagg 4801 gctaccaggg tggagtgcac caccatcgtg aacggcgtgc gcaggtcctt ctacgtgtac 4861 gccaacggcg gcaagggatt ctgcaagctg cacaactgga actgcgtgaa ctgcgacacc 4921 ttctgcgctg gctccacctt catctctgac gaggtggcca gagacctgag cctgcagttc 4981 aagcgtccaa tcaaccccac cgaccagagc tcctacatcg tggactctgt gaccgtgaag 5041 aacggtagca tccacctgta cttcgacaag gctggccaga agacctacga gcgccactct 5101 ctgagccact tcgtgaacct ggacaacctg agggccaaca acaccaaggg cagcctgcct 5161 atcaacgtga tcgtgttcga cggaaagtcc aagtgcgagg aatctagcgc caagtccgcc 5221 agcgtgtact acagccagct gatgtgccag ccaatcctgc tgctggacca ggctctggtg 5281 tctgacgtgg gagacagcgc cgaagtggcc gtgaagatgt tcgacgccta cgtgaacacc 5341 ttctcctcta ccttcaacgt gcctatggag aagctgaaga ccctggtggc tactgctgag 5401 gctgaactgg ctaagaacgt gtccctggac aacgtgctga gcaccttcat ctccgccgct 5461 aggcagggtt tcgtggactc tgacgtggaa accaaagacg tggtggagtg cctgaagctg 5521 tctcaccaga gcgacatcga agtgaccggc gactcctgca acaactacat gctgacctac 5581 aacaaggtgg agaacatgac cccaagagac ctgggagctt gcatcgactg ctccgcccgt 5641 cacatcaacg ctcaggtggc caagtctcac aacatcgccc tgatctggaa cgtgaaggac 5701 ttcatgagcc tgtccgagca gctgcgcaag cagatcagga gcgccgctaa gaagaacaac 5761 ctgcccttca agctgacctg cgctaccacc agacaggtgg tgaacgtggt gaccaccaag 5821 atcgccctga agggcggact cgagctcaag cttcgaattc tgcagtcgac ggtaccrcgg 5881 gcccgggatc ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 5941 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 6001 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 6061 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 6121 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 6181 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 6241 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 6301 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 6361 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 6421 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 6481 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 6541 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 6601 tacaagtag //