LOCUS Exported 9637 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro. ACCESSION . VERSION . KEYWORDS pLenti CMV Puro LUC (w168-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9637) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9637) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9637 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 505..529 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 613..637 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 643..2295 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(709..727) /label=LucNrev /note="Firefly luciferase, reverse primer" protein_bind complement(2302..2326) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 2357..2945 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2410..2430) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2828..2839) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 2959..3458 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(2994..3017) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 3391..3410 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 3479..4078 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3479..3498) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3975..3995 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 4216..4449 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4521..4642 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4558..4577) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4612..4631 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4682..4817 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4744..4763 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4837..4856) /label=T7 /note="T7 promoter, forward primer" promoter complement(4838..4856) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4866..4883) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4866..4882) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4875..4897) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5024..5479 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5111..5130) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5321..5342 /label=F1ori-F /note="F1 origin, forward primer" promoter 5505..5609 /gene="bla" /label=AmpR promoter CDS 5610..6470 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5828..5847) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6641..7229 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7130..7149 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7383..7400 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7517..7538 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7553..7583 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7591..7607 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7596..7618 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7615..7631 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7615..7631 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7650..7670 /label=T3 /note="T3 promoter, forward primer" promoter 7652..7670 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7698..7924 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7925..8105 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8152..8277 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8770..9003 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9188..9232 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9499..9615 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg 541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt 601 ctgcagatat caacaagttt gtacaaaaaa gcaggctcca ccatggaaga cgccaaaaac 661 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg 721 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat 781 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct 841 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt 901 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac 961 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg 1021 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc 1081 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg 1141 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc 1201 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa 1261 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt 1321 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt 1381 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat 1441 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg 1501 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta 1561 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg 1621 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca 1681 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt 1741 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa 1801 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa 1861 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg 1921 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc 1981 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac 2041 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt 2101 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta 2161 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt 2221 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga 2281 aagatcgccg tgtaattcta gacccagctt tcttgtacaa agtggttgat atccagcaca 2341 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 2401 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 2461 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 2521 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 2581 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 2641 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 2701 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 2761 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 2821 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 2881 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 2941 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 3001 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 3061 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 3121 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 3181 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 3241 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 3301 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 3361 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 3421 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 3481 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 3541 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 3601 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 3661 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 3721 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 3781 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 3841 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 3901 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 3961 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 4021 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg 4081 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg 4141 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 4201 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg 4261 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 4321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 4381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 4441 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 4501 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 4561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4621 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 4681 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 4741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 4801 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 4861 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 4921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 4981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 5041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 5101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 5161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 5221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 5281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 5341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 5401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 5461 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 5521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 5581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 5641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 5701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 5761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 5821 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 5881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 5941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 6001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 6061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 6121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 6181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 6241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 6301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 6361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 6421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 6481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 6541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 6601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 6661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 6721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 6781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 6841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 6901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 6961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 7021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 7081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 7141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 7201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 7261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 7321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 7381 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 7441 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 7501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 7561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 7621 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc 7681 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac 7741 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga 7801 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg 7861 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat 7921 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 8041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 8101 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga 8161 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt 8221 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt 8281 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag 8341 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt 8401 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca 8461 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat 8521 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa 8581 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg 8641 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga 8701 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 8761 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 8821 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 8881 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 8941 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 9001 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 9061 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 9121 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 9181 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 9241 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 9301 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 9361 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 9421 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 9481 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga 9541 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac 9601 aaaattcaaa attttatcga taagcttggg agttccg //