LOCUS       Exported                7468 bp ds-DNA     circular SYN 23-SEP-2022
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes for production of 
            serotype 2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV2/2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7468)
  TITLE     AAV Packaging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        555..585
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     683..699
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     683..699
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    713..760
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4850..4861
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5336..5353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5336..5352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5345..5367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5493..5948)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5631..5652)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5842..5861
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5975..6079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6080..6940
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6298..6317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7111..231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgccgatatc
      661 gttaacgccc cgcgccggcc gctctagaac tagtggatcc cccggaagat cagaagttcc
      721 tattccgaag ttcctattct ctagaaagta taggaacttc tgatctattc gagctcggta
      781 cccctagagt cctgtattag aggtcacgtg agtgttttgc gacattttgc gacaccatgt
      841 ggtcacgctg ggtatttaag cccgagtgag cacgcagggt ctccattttg aagcgggagg
      901 tttgaacgcg cagccgccat gccggggttt tacgagattg tgattaaggt ccccagcgac
      961 cttgacgagc atctgcccgg catttctgac agctttgtga actgggtggc cgagaaggaa
     1021 tgggagttgc cgccagattc tgacatggat ctgaatctga ttgagcaggc acccctgacc
     1081 gtggccgaga agctgcagcg cgactttctg acggaatggc gccgtgtgag taaggccccg
     1141 gaggcccttt tctttgtgca atttgagaag ggagagagct acttccacat gcacgtgctc
     1201 gtggaaacca ccggggtgaa atccatggtt ttgggacgtt tcctgagtca gattcgcgaa
     1261 aaactgattc agagaattta ccgcgggatc gagccgactt tgccaaactg gttcgcggtc
     1321 acaaagacca gaaatggcgc cggaggcggg aacaaggtgg tggatgagtg ctacatcccc
     1381 aattacttgc tccccaaaac ccagcctgag ctccagtggg cgtggactaa tatggaacag
     1441 tatttaagcg cctgtttgaa tctcacggag cgtaaacggt tggtggcgca gcatctgacg
     1501 cacgtgtcgc agacgcagga gcagaacaaa gagaatcaga atcccaattc tgatgcgccg
     1561 gtgatcagat caaaaacttc agccaggtac atggagctgg tcgggtggct cgtggacaag
     1621 gggattacct cggagaagca gtggatccag gaggaccagg cctcatacat ctccttcaat
     1681 gcggcctcca actcgcggtc ccaaatcaag gctgccttgg acaatgcggg aaagattatg
     1741 agcctgacta aaaccgcccc cgactacctg gtgggccagc agcccgtgga ggacatttcc
     1801 agcaatcgga tttataaaat tttggaacta aacgggtacg atccccaata tgcggcttcc
     1861 gtctttctgg gatgggccac gaaaaagttc ggcaagagga acaccatctg gctgtttggg
     1921 cctgcaacta ccgggaagac caacatcgcg gaggccatag cccacactgt gcccttctac
     1981 gggtgcgtaa actggaccaa tgagaacttt cccttcaacg actgtgtcga caagatggtg
     2041 atctggtggg aggaggggaa gatgaccgcc aaggtcgtgg agtcggccaa agccattctc
     2101 ggaggaagca aggtgcgcgt ggaccagaaa tgcaagtcct cggcccagat agacccgact
     2161 cccgtgatcg tcacctccaa caccaacatg tgcgccgtga ttgacgggaa ctcaacgacc
     2221 ttcgaacacc agcagccgtt gcaagaccgg atgttcaaat ttgaactcac ccgccgtctg
     2281 gatcatgact ttgggaaggt caccaagcag gaagtcaaag actttttccg gtgggcaaag
     2341 gatcacgtgg ttgaggtgga gcatgaattc tacgtcaaaa agggtggagc caagaaaaga
     2401 cccgccccca gtgacgcaga tataagtgag cccaaacggg tgcgcgagtc agttgcgcag
     2461 ccatcgacgt cagacgcgga agcttcgatc aactacgcag acaggtacca aaacaaatgt
     2521 tctcgtcacg tgggcatgaa tctgatgctg tttccctgca gacaatgcga gagaatgaat
     2581 cagaattcaa atatctgctt cactcacgga cagaaagact gtttagagtg ctttcccgtg
     2641 tcagaatctc aacccgtttc tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat
     2701 catatcatgg gaaaggtgcc agacgcttgc actgcctgcg atctggtcaa tgtggatttg
     2761 gatgactgca tctttgaaca ataaatgatt taaatcaggt atggctgccg atggttatct
     2821 tccagattgg ctcgaggaca ctctctctga aggaataaga cagtggtgga agctcaaacc
     2881 tggcccacca ccaccaaagc ccgcagagcg gcataaggac gacagcaggg gtcttgtgct
     2941 tcctgggtac aagtacctcg gacccttcaa cggactcgac aagggagagc cggtcaacga
     3001 ggcagacgcc gcggccctcg agcacgacaa agcctacgac cggcagctcg acagcggaga
     3061 caacccgtac ctcaagtaca accacgccga cgcggagttt caggagcgcc ttaaagaaga
     3121 tacgtctttt gggggcaacc tcggacgagc agtcttccag gcgaaaaaga gggttcttga
     3181 acctctgggc ctggttgagg aacctgttaa gacggctccg ggaaaaaaga ggccggtaga
     3241 gcactctcct gtggagccag actcctcctc gggaaccgga aaggcgggcc agcagcctgc
     3301 aagaaaaaga ttgaattttg gtcagactgg agacgcagac tcagtacctg acccccagcc
     3361 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag
     3421 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa
     3481 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg
     3541 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc
     3601 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt
     3661 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg
     3721 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga
     3781 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga
     3841 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc
     3901 agacgtcttc atggtgccac agtatggata cctcaccctg aacaacggga gtcaggcagt
     3961 aggacgctct tcattttact gcctggagta ctttccttct cagatgctgc gtaccggaaa
     4021 caactttacc ttcagctaca cttttgagga cgttcctttc cacagcagct acgctcacag
     4081 ccagagtctg gaccgtctca tgaatcctct catcgaccag tacctgtatt acttgagcag
     4141 aacaaacact ccaagtggaa ccaccacgca gtcaaggctt cagttttctc aggccggagc
     4201 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg
     4261 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa
     4321 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa
     4381 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc
     4441 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac
     4501 aaccaatccc gtggctacgg agcagtatgg ttctgtatct accaacctcc agagaggcaa
     4561 cagacaagca gctaccgcag atgtcaacac acaaggcgtt cttccaggca tggtctggca
     4621 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca
     4681 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct
     4741 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc
     4801 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctgca
     4861 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc
     4921 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg
     4981 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt
     5041 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga
     5101 taagtagcat ggcgggttaa tcattaacta cagcccgggc gtttaaacag cgggcggagg
     5161 ggtggagtcg tgacgtgaat tacgtcatag ggttagggag agtcctgtat tagaggtcac
     5221 gtgagtgttt tgcgacattt tgcgacacca tgtggtcacg ctgggtattt aagcccgagt
     5281 gagcacgcag ggtctccatt ttgagcggga ggtttgaacg agcgctggcg cgctcactgg
     5341 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5401 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5461 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa
     5521 attcgcgtta aatttttgtt aaatcagctc atttttttaa ccaataggcc gaaatcggca
     5581 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5641 acaagagtcc actattaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     5701 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     5761 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5821 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5881 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5941 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6061 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6121 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6181 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6241 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6301 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6361 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6421 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6481 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6541 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6601 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6661 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6721 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6781 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6841 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6901 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7441 gtgcacacag cccagcttgg agcgaacg
//
