LOCUS       Exported                7942 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression of Androgen receptor fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP-C1-AR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino 
            GN, Marcelli M, Weigel NL, Mancini MA
  TITLE     Polyglutamine-expanded androgen receptors form aggregates that 
            sequester heat shock proteins, proteasome components and SRC-1, and 
            are suppressed by the HDJ-2 chaperone.
  JOURNAL   Hum Mol Genet. 1999 May . 8(5):731-41.
  PUBMED    10196362
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      632..641
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             638..1354
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             638..1354
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(683..704)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(944..963)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1291..1312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4755..4876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4792..4811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4846..4865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4883..5338)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5020..5041)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5232..5251
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5365..5469
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5471..5828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(5494..5514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5679..5814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5741..5760
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5863..6657
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5917..5936)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6527..6546
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6845..6864)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6889..6936
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7265..7853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7754..7773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      661 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      721 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      781 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      841 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      901 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      961 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1021 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1081 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1141 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1201 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1261 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1321 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
     1381 ttcgaattct gcagtcgacg gtaccatgga agtgcagtta gggctgggaa gggtctaccc
     1441 tcggccgccg tccaagacct accgaggagc tttccagaat ctgttccaga gcgtgcgcga
     1501 agtgatccag aacccgggcc ccaggcaccc agaggccgcg agcgcagcac ctcccggcgc
     1561 cagtttgctg ctgctgcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     1621 gcagcagcag cagcagcagc aagagactag ccccaggcag cagcagcagc agcagggtga
     1681 ggatggttct ccccaagccc atcgtagagg ccccacaggc tacctggtcc tggatgagga
     1741 acagcaacct tcacagccgc agtcggccct ggagtgccac cccgagagag gttgcgtccc
     1801 agagcctgga gccgccgtgg ccgccagcaa ggggctgccg cagcagctgc cagcacctcc
     1861 ggacgaggat gactcagctg ccccatccac gttgtccctg ctgggcccca ctttccccgg
     1921 cttaagcagc tgctccgctg accttaaaga catcctgagc gaggccagca ccatgcaact
     1981 ccttcagcaa cagcagcagg aagcagtatc cgaaggcagc agcagcggga gagcgaggga
     2041 ggcctcgggg gctcccactt cctccaagga caattactta gggggcactt cgaccatttc
     2101 tgacaacgcc aaggagttgt gtaaggcagt gtcggtgtcc atgggcctgg gtgtggaggc
     2161 gttggagcat ctgagtccag gggaacagct tcggggggat tgcatgtacg ccccactttt
     2221 gggagttcca cccgctgtgc gtcccactcc ttgtgcccca ttggccgaat gcaaaggttc
     2281 tctgctagac gacagcgcag gcaagagcac tgaagatact gctgagtatt cccctttcaa
     2341 gggaggttac accaaagggc tagaaggcga gagcctaggc tgctctggca gcgctgcagc
     2401 agggagctcc gggacacttg aactgccgtc taccctgtct ctctacaagt ccggagcact
     2461 ggacgaggca gctgcgtacc agagtcgcga ctactacaac tttccactgg ctctggccgg
     2521 accgccgccc cctccgccgc ctccccatcc ccacgctcgc atcaagctgg agaacccgct
     2581 ggactacggc agcgcctggg cggctgcggc ggcgcagtgc cgctatgggg acctggcgag
     2641 cctgcatggc gcgggtgcag cgggacccgg ttctgggtca ccctcagccg ccgcttcctc
     2701 atcctggcac actctcttca cagccgaaga aggccagttg tatggaccgt gtggtggtgg
     2761 tgggggtggt ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
     2821 cgaggcggga gctgtagccc cctacggcta cactcggccc cctcaggggc tggcgggcca
     2881 ggaaagcgac ttcaccgcac ctgatgtgtg gtaccctggc ggcatggtga gcagagtgcc
     2941 ctatcccagt cccacttgtg tcaaaagcga aatgggcccc tggatggata gctactccgg
     3001 accttacggg gacatgcgtt tggagactgc cagggaccat gttttgccca ttgactatta
     3061 ctttccaccc cagaagacct gcctgatctg tggagatgaa gcttctgggt gtcactatgg
     3121 agctctcaca tgtggaagct gcaaggtctt cttcaaaaga gccgctgaag ggaaacagaa
     3181 gtacctgtgc gccagcagaa atgattgcac tattgataaa ttccgaagga aaaattgtcc
     3241 atcttgtcgt cttcggaaat gttatgaagc agggatgact ctgggagccc ggaagctgaa
     3301 gaaacttggt aatctgaaac tacaggagga aggagaggct tccagcacca ccagccccac
     3361 tgaggagaca acccagaagc tgacagtgtc acacattgaa ggctatgaat gtcagcccat
     3421 ctttctgaat gtcctggaag ccattgagcc aggtgtagtg tgtgctggac acgacaacaa
     3481 ccagcccgac tcctttgcag ccttgctctc tagcctcaat gaactgggag agagacagct
     3541 tgtacacgtg gtcaagtggg ccaaggcctt gcctggcttc cgcaacttac acgtggacga
     3601 ccagatggct gtcattcagt actcctggat ggggctcatg gtgtttgcca tgggctggcg
     3661 atccttcacc aatgtcaact ccaggatgct ctacttcgcc cctgatctgg ttttcaatga
     3721 gtaccgcatg cacaagtccc ggatgtacag ccagtgtgtc cgaatgaggc acctctctca
     3781 agagtttgga tggctccaaa tcacccccca ggaattcctg tgcatgaaag cactgctact
     3841 cttcagcatt attccagtgg atgggctgaa aaatcaaaaa ttctttgatg aacttcgaat
     3901 gaactacatc aaggaactcg atcgtatcat tgcatgcaaa agaaaaaatc ccacatcctg
     3961 ctcaagacgc ttctaccagc tcaccaagct cctggactcc gtgcagccta ttgcgagaga
     4021 gctgcatcag ttcacttttg acctgctaat caagtcacac atggtgagcg tggactttcc
     4081 ggaaatgatg gcagagatca tctctgtgca agtgcccaag atcctttctg ggaaagtcaa
     4141 gcccatctat ttccacaccc agtgaagcat tggaaaccct atttccccac cccagctcat
     4201 gccccctttc agatgtcttc tgcctgttat aactctgcac tactcctctg cagtgccttg
     4261 gggaatttcc tctattgatg tacagtctgt catgaacatg ttcctgaatt ctatttgctg
     4321 ggcttttttt ttctctttct ctcctttctt tttcttcttc cctccctatc taaccctccc
     4381 atggcacctt cagactttgc ttcccattgt ggctcctatc tgtgttttga atggtgttgt
     4441 atgcctttaa atctgtgatg atcctcatat ggcccagtgt caagttgtgc ttgtttacag
     4501 cactactctg tgccagccac acaaacgttt acttatctta tgccacggga agtttagaga
     4561 gctaagatta tctggggaaa tcaaaacaaa aacaagcaaa caaaaaaaaa agaaaaacgg
     4621 aattcagatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     4681 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4741 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4861 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     4921 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4981 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5041 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5101 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5161 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5221 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5281 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     5341 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5401 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5461 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     5521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5581 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5641 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5701 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5761 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5821 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5881 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5941 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6001 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     6061 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6121 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6181 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6241 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6301 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6361 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6421 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6481 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6541 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6601 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6661 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6721 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6781 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     6841 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     6901 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     6961 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7021 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7081 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     7141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7921 ctgcgttatc ccctgattct gt
//
