LOCUS Exported 5352 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization of mGreenLantern fluorescent protein to clathrin. ACCESSION . VERSION . KEYWORDS Clathrin-mGreenLantern SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5352) AUTHORS Campbell BC, Nabel EM, Murdock MH, Lao-Peregrin C, Tsoulfas P, Blackmore MG, Lee FS, Liston C, Morishita H, Petsko GA TITLE mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging. JOURNAL Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30710-30721. doi: 10.1073/pnas.2000942117. Epub 2020 Nov 18. PUBMED 33208539 REFERENCE 2 (bases 1 to 5352) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5352 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..204 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 155..175 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 243..252 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 249..965 /codon_start=1 /product="monomeric GFP derivative that displays exceptionally high brightness in cells (Campbell et al., 2020)" /label=mGreenLantern /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK ERVTAAGITHDMDELYK" primer_bind complement(294..315) /label=EGFP-N /note="EGFP, reverse primer" polyA_signal 1776..1897 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1813..1832) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1867..1886 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1904..2359) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2041..2062) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2253..2272 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2386..2490 /gene="bla" /label=AmpR promoter promoter 2492..2849 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2515..2535) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2700..2835 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2762..2781 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2884..3678 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2938..2957) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3548..3567 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3866..3885) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3910..3957 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4286..4874 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4775..4794 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 5049..5352 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg 241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 301 agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc gagggcgatg 361 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 421 ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc taccccgacc 481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 541 ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg 601 acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac ggcaacatcc 661 tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg gccgacaagc 721 agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac ggcggcgtgc 781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 841 acaaccacta cctgagccat cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc 901 acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg gacgagctgt 961 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac atggctgatg 1021 actttggctt cttctcgtcg tcggagagtg gtgccccgga ggcggcggag gaggacccgg 1081 cggccgcctt cctggcccag caggagagcg agattgcagg catagagaac gacgagggct 1141 tcggggcacc tgccggcagc catgcggccc ccgcacagcc gggccccacg agtggggctg 1201 gttctgagga catggggacc acagtcaatg gagatgtgtt tcaggaggcc aacggtcctg 1261 ctgatggcta cgcagccatt gcccaggctg acaggctgac ccaggagcct gagagcatcc 1321 gcaagtggcg agaggagcag aggaaacggc tgcaagagct ggatgctgca tctaaggtca 1381 cggaacagga atggcgggag aaggccaaga aggacctgga ggagtggaac cagcgccaga 1441 gtgaacaagt agagaagaac aagatcaaca accgggcatc cgaggaggct ttcgtgaagg 1501 aatccaagga ggagacccca ggcacagagt gggagaaggt ggcccagcta tgtgacttca 1561 accccaagag cagcaagcag tgcaaagatg tgtcccgcct gcgctcggtg ctcatgtccc 1621 tgaagcagac gccactgtcc cgctagggat ccaccggatc tagataactg atcataatca 1681 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 1741 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 1801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 1861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat 1921 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 1981 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 2041 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 2101 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 2161 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 2221 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 2281 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 2341 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 2401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 2461 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 2521 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2581 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 2641 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2701 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2761 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2821 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 2881 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 2941 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 3001 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 3061 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 3121 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 3181 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 3241 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 3301 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 3361 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 3421 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 3481 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 3541 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 3601 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3661 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 3721 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3841 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 3901 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 3961 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 4021 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 4081 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 4141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 4321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 4381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 4441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 4501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 4561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 4621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 4681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 4741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 4801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 4861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 4921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 4981 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 5041 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 5101 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 5161 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 5221 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 5281 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 5341 gctattacca tg //