LOCUS       Exported                 719 bp ds-DNA     linear   SYN 04-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pdestDD04Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Aug 22. ():.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    287..386
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             395..415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(458..486)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaan nnnncgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
      181 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca
      241 aagtgcgtcg ggtgatgctg ccaacttagt cgacgctcta gagctccaaa taatgatttt
      301 attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat
      361 gccaactttg tacaaaaaag caggcttgac tgctccaaag aagaagcgta aggtaccggt
      421 agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      481 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      541 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt
      601 aagtttaaac atatatatac taactaaccc tgattattta aattttcagc caacacttgt
      661 cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggc
//
