LOCUS       Exported               11561 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pBbdCas9H(-10TC)_arr2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        591..635
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    611..627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    643..659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             678..689
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             696..4802
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4990..6009
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6621..6640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatgtactg ctgccttaca agcctctaca cctgatctct caaccattga tacaacaacg
       61 cttgcaccaa gttgatcagc tctcgattta attgcggcta caatttgact aggcgcatgt
      121 aatgcaagtg atgatgtggc aactccaatt aaaagtgatt gtttccccgc aagttgctga
      181 gcaacgcgat ttggtatata attcaactct gccatagctg cttcaacctt ctctcttgtt
      241 tttgcagata catgacttgc ttggttcaca actctcgaaa ccgtttggta gctaaccccg
      301 gcatattcag caacatcata caaagtaaca ggtttcatgg atcattcctc catgataaaa
      361 tttaaatttc tgactttggc aaatcctttt gcctgttaaa aagaatagca caaaaattaa
      421 aaaaaaagaa acgcaaatag caatagcatt aaaaataatt attattattt atttctttga
      481 ttaaaatcaa aagcctttaa tgtattttct tgatctttca tgcgcttaac ggtaaaatcc
      541 aaggtattaa gttcaccttc cacttttctc tctctatctt ctctagaaaa tcataaaaaa
      601 tttatttgct ttgtgagcgg ataacaatta taacagattc aattgtgagc ggataacaat
      661 ttcacacaga attcattaaa gaggagaaat tacatatgga taagaaatac tcaataggct
      721 tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat aaggttccgt
      781 ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa aatcttatag
      841 gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa cggacagctc
      901 gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt ttttcaaatg
      961 agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt ttggtggaag
     1021 aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa gttgcttatc
     1081 atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct actgataaag
     1141 cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt ggtcattttt
     1201 tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt atccagttgg
     1261 tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga gtagatgcta
     1321 aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc attgctcagc
     1381 tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca ttgggtttga
     1441 cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag ctttcaaaag
     1501 atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa tatgctgatt
     1561 tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc ctaagagtaa
     1621 atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac gatgaacatc
     1681 atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa aagtataaag
     1741 aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg ggagctagcc
     1801 aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt actgaggaat
     1861 tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt gacaacggct
     1921 ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga caagaagact
     1981 tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact tttcgaattc
     2041 cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg actcggaagt
     2101 ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt gcttcagctc
     2161 aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa aaagtactac
     2221 caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca aaggtcaaat
     2281 atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag aaagccattg
     2341 ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa gaagattatt
     2401 tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat agatttaatg
     2461 cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat tttttggata
     2521 atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta tttgaagata
     2581 gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat aaggtgatga
     2641 aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa ttgattaatg
     2701 gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca gatggttttg
     2761 ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa gaagacattc
     2821 aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca aatttagctg
     2881 gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat gaattggtca
     2941 aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt gaaaatcaga
     3001 caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca
     3061 aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa ttgcaaaatg
     3121 aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac caagaattag
     3181 atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt ttccttaaag
     3241 acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt aaatcggata
     3301 acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg
     3361 ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt ggaggtttga
     3421 gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc caaatcacta
     3481 agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa aatgataaac
     3541 ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac ttccgaaaag
     3601 atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat gatgcgtatc
     3661 taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa tcggagtttg
     3721 tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa
     3781 taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc ttcaaaacag
     3841 aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact aatggggaaa
     3901 ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa gtattgtcca
     3961 tgccccaagt caatattgtc aagaaaacag aagtccagac aggcggattc tccaaggagt
     4021 caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac tgggatccaa
     4081 aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg gttgctaagg
     4141 tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg atcacaattc
     4201 tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa ggatataagg
     4261 aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag ttagaaaacg
     4321 gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag ctggctctgc
     4381 caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg aagggtagtc
     4441 cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat ttagatgaga
     4501 ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc aatttagata
     4561 aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa gcagaaaata
     4621 ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa tattttgata
     4681 caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc actcttatcc
     4741 atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta ggaggtgact
     4801 aataaaagct tgatcagatc ccagcttttt tttgaagtgc ctggcagtaa gttgaaaaaa
     4861 tttgaaataa acttggacta tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg
     4921 aattattata taatttagtt aaaagcaatt ttaaatgagg gaggtttcca tcatatggat
     4981 agaagtggta aacctgaatt aactgctact agtgttgaaa aatttttaat tgaaaaattt
     5041 gatagtgtta gtgatttaat gcaattaagt gaaggtgaag aaagtagagc ttttagtttt
     5101 gatgttggtg gtagaggtta tgttttaaga gttaatagtt gtgctgatgg tttttataaa
     5161 gatagatatg tttatagaca ttttgctagt gctgctttac ctattcctga agttttagat
     5221 attggtgaat ttagtgaaag tttaacttat tgtattagta gaagagcaca aggtgttact
     5281 ttacaagatt tacctgaaac tgaattacct gctgttttac aacctgttgc tgaagctatg
     5341 gatgctattg cggcggctga tctcagtcaa acttcgggtt ttggtccttt tggtcctcaa
     5401 ggtattggtc aatatactac ttggagagat tttatttgtg ctattgctga tcctcatgtt
     5461 tatcattggc aaactgttat ggatgatact gttagtgcta gtgttgctca agcattagat
     5521 gaattaatgt tatgggctga agattgtcct gaagttagac atttagttca tgctgatttt
     5581 ggtagtaata atgttttaac tgataatggt agaattactg ctgttattga ttggagtgaa
     5641 gctatgtttg gtgatagtca atatgaagtt gctaatattt ttttttggag accttggtta
     5701 gcttgtatgg aacaacaaac tagatatttt gaaagaagac atcctgaatt agctggtagt
     5761 cctagattaa gagcttatat gttaagaatt ggtttagatc aattatatca aagtttagtt
     5821 gatggtaatt ttgatgatgc tgcttgggct caaggtagat gtgatgctat tgttagaagt
     5881 ggtgctggta ctgttggtag aactcaaatt gctagaagaa gtgctgctgt ttggactgat
     5941 ggttgtgttg aagttttagc tgatagtggt aatagaagac ctagtactag acctagagct
     6001 aaagaataat gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcatg
     6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6781 ttctttcctg cactagatcc ccggcaagtt catccttagg gttttcatat ctcttaaaaa
     6841 gtcaatttct gcttgattaa tttcttgtgg attattgttt ttgcggtttt tcatttttta
     6901 tctccttgag tattgaattt tttcttatat ataaatatat agcaaaaact atttttgcca
     6961 acttttttac aaaaaatttt ttattaaaac acttagggct ttgctaaatt ctcttttaaa
     7021 agaacttagt aaagccctat ggatttaaga actgattata cttacgtagt aaaaatacta
     7081 cagatattaa tagtatagac catatcaaaa tcataatcaa tttatattta tctaaaattg
     7141 tagtccatac aaaatacaaa aaaattaggt ttttctaaat tctattttaa aagaacttag
     7201 taacacctaa tctctaaatt acttacaaaa ttctaaaatt agatttttag ttcttcatat
     7261 ttctttaaaa gttttttaag aaaatctttt tgattttcaa aaatttcatg catcataaaa
     7321 ctagtaaatt tggatttttt tttgtaaaaa tcataacttt cttgggtttt aagttgaaat
     7381 cttaatggtt ttattggatt ttgcctagat ttttttaacg ccggactttc tttatctttt
     7441 ataaaattat aagaattttt aataccattt tctattaaat aatcttcttt tataagcccc
     7501 tcttgcagtg cagttgctaa tcttaaatac ttatacgctt gagtttttgc tacatcaaag
     7561 tcttttataa aatcaagaaa agttttataa ccatcaaatt tataatattg attatctttt
     7621 atttctttta aaattttaat attttccacc ctgtggaata tttctttttt gaaattgtca
     7681 tttaactttt cctttagtgt cttgtaataa gaaattttac tttcattaat cattaaattt
     7741 tgttcaacat aagtatctat cttttttgtt aaaactttaa caacacgttt tttttccata
     7801 aaaattttcc tttatttttg caacattttc cttaattgat ataattgtta ctattttaga
     7861 ttttttaata tccacttaag aattaatccc tcactagaat attttttatt atagaattca
     7921 tatacatgtt tttctaaatc tataaattta tcaacaattg ctttattata gggtgtatct
     7981 atcttttctt ttcttaataa tcgtctcata ttctttaaat aacaacaaac actgccggtt
     8041 ttaaacctaa attctatgta atatattttt gaaaaagtat atacattttc aaccccgttt
     8101 atttcatact ttacaaaaac attatttatt ggttttctat aaccatagta aatgcctaaa
     8161 aatttgtccc cgtctttcat aggatataga taaagttttt gaatctctga tttatctaat
     8221 aatttttcaa aaaaaattat acaccgcttt tgtttgttac taactactct atgcaatgcc
     8281 tttaatattt ttgtgtgata tatattttta tcaccttttt tttctttcag caggaagata
     8341 ctttcttttg gaaatttaat tttttgttca tttattcttg cagccttaag tccgattttc
     8401 atatttaatc cttatatagc ctttctattt aattctttta aactcaataa acttttttgg
     8461 tttttcatta gttctaataa ttcgcaataa tatgtgcaaa acactttatt gtattctatt
     8521 ttcttttggt tattcaaata ttcttttata attggcttta gaagttcaat atttacttct
     8581 ttttttagtt gttcaataag gatattgaaa atgtttgttt ttaaatcttg ataattttgt
     8641 tgagaatttt ctttttttct ttcaattgac ttctctaatt tacgctttat attgtttaaa
     8701 tcgctatatt tatgactttc aataataaaa tgaggcttaa atttgtaatt ttcgtatatt
     8761 ttttggaaac ttgtttctaa ttgtttagaa ttatattcat ttttttcaaa ttctttttga
     8821 gtgttgcata gaatttcctt taatttttct tgtttttctt tcatacaaga tttagtaaaa
     8881 tgtatatttt tattttttag taatttaatt tctattattt ttatgatttt aagtattttg
     8941 attatattat ctttatcaat atttaaattg aataaaattg taagaaaaat tgaaagaatt
     9001 tttttacata aaaagttaca tttattgaag taatttctta cttgatactt ttctatttca
     9061 ttaatctttc tttcttcttt tatattattt ttattattta aacactcctt taaatctaca
     9121 ctcccatttt tagtaaatct atctttaagg ccccttgaaa ctctattttg aaatcttaaa
     9181 tctttttttt cttgaaagta tttatagatt ttttggtaac attctttttt tgaaaaatta
     9241 agttgatagt atatttcggt ccccaaattt acccctaagt gtttgtgata attagttgtg
     9301 actttaaatt ctttttctaa tttgtaaagg taattttgca gtgtttttag tttaacgggt
     9361 gtttgactat ttcttttaag gttttcatta aagtaataga gtatgttttt ttgtgtgtat
     9421 ttcttatatg ttttatttat gtacgctagt gttgagataa ggactactaa tttatactgt
     9481 aatttattgt agcaagttgt ggttttactg gttttgttta tattagataa gttttccatt
     9541 tatagctcct tgaataccgg attttttatc tataaagtat atatatcaca attttgattt
     9601 aaaagtaaat tcttttttta aaaatacaaa aattttttat aaattatata gaccatgata
     9661 ttgtattaat actaattgat tttaatttaa ggttgaacta aaataaacat agttttgtag
     9721 aaaataattt ttcattattt tgctacttga tattggatcg taaaaatatt agggctttac
     9781 aaagttcttt taaaagagaa tttagtaaag ccctaagtct tttaataaaa aattttttgt
     9841 aaaaaagttg gcaaaaatag tttttgctat atatttatat ataagaaaaa attcaatact
     9901 caaggagata aaaaatggaa aacttaaaca ataatccaca aaaaattaat caaataaata
     9961 agcaaactaa ttttattggt tttgaaaatt ttatagaact tgataatcaa gttaatcaaa
    10021 ctaaagaaaa aagtaaatta agtaagatag gtaagaaatt acccggaata agcagtcaag
    10081 tctagtgcgg ccgcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
    10141 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
    10201 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg aggtaacgtc
    10261 atggtaaaag attggattcc tatttctcat gataattata aacaagtaca aggaccattt
    10321 tatcatggaa ctaaagctaa tttagcaatt ggtgatttac taactacagg atttatttct
    10381 cattttgaag atggtcgaat tcttaaacat atttattttt cagctttaat ggaaccagca
    10441 gtttggggag ctgaacttgc tatgtcacta tctggtcttg aaggtcgtgg ttatatatat
    10501 atagttgaac caacaggacc atttgaagat gatccaaatc ttacaaataa aaaatttcct
    10561 ggtaatccaa cacaatctta tagaacttgt gaacctttaa gaattgttgg tgttgttgaa
    10621 gattgggaag gacatcctgt tgaattaata agaggaatgt tagattcatt agaagattta
    10681 aaacgtcgtg gtttacatgt tattgaagat taacctggcg cgccaaataa tgcccgtgtc
    10741 cctgttgaat agggacacgg ttttttaaca atttttttat tcctaggcct tagtggccgg
    10801 tacccgggga tccttattac tggccgcttt ctagcctgga tacttgtctg gctagttgca
    10861 ttagagagtc agctagagcc ctagggctag ctgtttgtgt attcggagcc agtgtagttt
    10921 ttctttttac taacgatacg ggtaataatt gattgccttt tacagcctga ccttgagaca
    10981 attgcaaaag tctgtccaca gaggtttgac ctaataatct aaaatcttgt ttgatagtag
    11041 taagtggagg tatgtagcaa ctagaatctt ctgtatcatc ataacccaca actgaaatat
    11101 cagccccaac tctaagtcca ctttcagtaa tagctcgcat agctcctaaa gccatttgat
    11161 cattagcaac taacatagca gtagggacaa tgccttcatt taacatttgc attgtttgct
    11221 gaaatcctga catagcagac caatctcctt ctctttctgc tattggttgt atttgatttc
    11281 ttgttaaata cttgtgccat cctgctaatc tcaatctggc acttactgaa cttaagggac
    11341 ctgcaagtag tgcaatttgt tgatggccta atgcaacaag gtgttcaact cctaaccttg
    11401 ttccatcttc gtgagaaaaa attatagagt ttattggggt ttggtccgac acatccaaaa
    11461 acaatgctgg aacattagta catgctgctt caactgcaat cgcatcttgg tcatcaagag
    11521 gataattaat tataagtcca ctaacacgtt gagctaataa a
//
