LOCUS Exported 9670 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses EGFP-tagged inner mitochondrial membrane marker. ACCESSION . VERSION . KEYWORDS pLVX-EF1a-CCO-IRES-Puromycin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9670) AUTHORS Schormann W, Hariharan S, Andrews DW TITLE A reference library for assigning protein subcellular localizations by image-based machine learning. JOURNAL J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. PUBMED 31968357 REFERENCE 2 (bases 1 to 9670) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9670 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3575..3649 /codon_start=1 /product="mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989)" /label=COX8 presequence /translation="MSVLTPLLLRGLTGSARRLPVPRAK" regulatory 3677..3686 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3683..4402 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3683..4399 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3728..3749) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3989..4008) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4336..4357 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4420..4993 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4587..4604) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4814..4833 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5026..5625 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5026..5045) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5522..5542 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5639..6227 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5692..5712) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6110..6121) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6434..7067 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7195..7211) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7195..7211) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7208..7230) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7219..7235 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7243..7273) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7288..7309 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7426..7443) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7597..8185) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7677..7696) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8356..9216) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8979..8998 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9217..9321) /gene="bla" /label=AmpR promoter polyA_signal 9369..9503 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9406..9425) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9460..9479 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 ctcgagacta gttctagagc ggccgcaccg gatcatgtcc gtcctgacgc cgctgctgct 3601 gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga tccattcgtt 3661 gggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 3721 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 3781 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3841 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3901 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3961 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 4021 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 4081 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 4141 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 4201 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 4261 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 4321 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 4381 catggacgag ctgtacaagt aaagcggccg cggatcccgc ccctctccct cccccccccc 4441 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt 4501 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt 4561 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt 4621 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct 4681 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt 4741 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt 4801 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa 4861 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 4921 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa 4981 aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag 5041 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc 5101 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg 5161 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 5221 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 5281 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 5341 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 5401 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 5461 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 5521 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 5581 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg tctggaacaa 5641 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5701 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5761 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5821 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5881 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5941 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 6001 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 6061 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6121 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6181 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc 6241 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 6301 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 6361 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 6421 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 6481 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg 6541 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag 6601 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg 6661 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat 6721 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac 6781 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg 6841 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt 6901 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6961 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7021 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7081 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc 7141 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata 7201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 8041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc 9361 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 9421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 9481 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 9541 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct 9601 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 9661 acttagtagt //