LOCUS Exported 8190 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pHes1(2.5k)-luc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8190) AUTHORS Takebayashi K, Sasai Y, Sakai Y, Watanabe T, Nakanishi S, Kageyama R TITLE Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements. JOURNAL J Biol Chem. 1994 Feb 18;269(7):5150-6. PUBMED 7906273 REFERENCE 2 (bases 1 to 8190) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8190 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2675..4327 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(2741..2759) /label=LucNrev /note="Firefly luciferase, reverse primer" intron 4568..4633 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4763..4783 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5208..5342 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5484..5501) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5655..6243) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5735..5754) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6414..7274) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7037..7056 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7275..7379) /gene="bla" /label=AmpR promoter rep_origin 7406..7861 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7493..7512) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7703..7724 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 8051..8172 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 aacataaccc atcaagctta acgtatgact aattgttctg gattctctac atccttttac 61 ttctgtctac tgcctctttt aaaggcactc ccaggggaat cttgtgggga atctatagat 121 ggcagccata ccacacagat acctggcaac gagctttcta aaggcactgt taatgaacta 181 cacccgatga aggcttcatg acctaaaggc acccctccgg attcaacccc ccgcaacacg 241 atctaagccc ccaaagggaa aaacagaatc cccaagcatt gtccagaaag cccggttctg 301 tgcgcccctg cagtaggttg gagaataaat actccgtggg agcggccact ctatctctca 361 tcgtggtaat cccagggttt aacagtgtct ggcgttccat cacaaagggg ggtggggggg 421 gcttgcccaa ggtcatgcag tcaaggaagc accacctcgt ccgggaccag agctggagaa 481 atctttcaca tgatctcagt gggaggttgg gggaggggac gaagggacga gccggtgcgc 541 cttgtgtgcc aggcctcccg gacgcgggaa agactggggg cggggatcaa acaacgcgct 601 gggatcccga gctgccccga gttcacggcg gcgggaaaac tgaggggtct ggggcacccg 661 ggtggaggcc agggcaggca ggagggaggc aggcagccgc ccgggcagtg cgcaccgccc 721 gcgccgcgcg gcaaatcccc cagggaggcc gccgccagag ccctggcgct cccggcccgg 781 cgcccgccac gtggcacccg gcggcggcgg cgactcccgg cgcgcagccc cggccagccg 841 gcagcacgca gccgcccgtc tcttcccagc cgtccgccgg cagcctcctg gacgcagcga 901 gggacccggg gaaggggcgg gcggcagcga agccgctgga accgcaagcc gccgagcgga 961 ggcaggaggc tgacccggcc gcttcctaag tgcggtcagg catctcgtga ccgcggcggg 1021 cgcccggacc cctcccccgc cgcagtcacc cgggtggttt cggccacgct cagatcccca 1081 agcccggagt cccggcgggc tgggggtggg gggtggcgat ggggggtggg gacggggaag 1141 ggggcccacg aattccccga tcgccaccct tgggcgtgcg ctccctgccc ggaaggattg 1201 accttggccg gggcggggga agggaggcca gcgcgggggc tcagctttcg ggccgccgcc 1261 cccgcggatt cctggctcgc ccgggccgga ggagggggcc ccggacacac ggacgcacgc 1321 acacacccca cacgcagcgc gcgccaatga gccctggcca ggcgcagggg gcggggtttt 1381 cccaggctcg agcacagcag ctgctattta ccttcttggc ttctcccggg gaccagaggc 1441 ggcccccgct ccctcccggc cggccgccgc cggcccccgc ctccctgccc ccggggcgcg 1501 cgcacacaca ctcacacacg cacgcacaca cacacatcct ctgggccccg gccgcagccg 1561 cggcggcaat aaaacatcct ggcacgtgct ccgactccag gcgcgccctc gccggccggc 1621 tgatgtcaaa ctgcagctcg gctggtggag ctcttaaagg gctcgcgcgc ggggcgccga 1681 ggccgccttg gccgcccgcg gggtgaggag gggagagccc cgactccctt gcccgccgtc 1741 tatccgtatt gccaactggg agcctgcggg gagaaaggta gagagagtgg cggaggaacg 1801 agcgcgcccc tcctgtcgca ttgctatcaa agcccccctt gttcattttt tttttcgttt 1861 gaaattccat ttcctttctg cccagtagga aattattctg attgttttct ggagaagcgt 1921 gaggcccaag gttgcaattc caagagagga atgaatgggc tagagggaga caagcatcca 1981 aaagacacct tgctccgaaa accctggctg tgaaggcatt tggggggacg gtaagggcat 2041 gtttagcgtg tggggtttag tatctcccca acctcatgag cagttttgac tcccctaaac 2101 atacagagtt cgagcgggaa ttccggaaga cattagagta acacatcctc tttaccttgt 2161 tccctccttt tttcaatcac taaattttgt cttggcctat atctgttcaa aatatttttc 2221 aaatgaactt attatacaaa gtagttatat tgcatgcagc aagaacaata aaaaccaaag 2281 gcctggccac aaaagaaata gactagacta aaactaagca aagcccagag gaaagagtta 2341 gcaaagggtt aaaatccttt tgattgacgt tgtagcctcc ggtgccccgg gctcaggcgc 2401 gcgccattgg ccgccagacc ttgtgcctag cggccaatgg gggggcgcag tccacgagcg 2461 gtgccgcgtg tctcttcctc ccattggctg aaagttactg tgggaaagaa agtttgggaa 2521 gtttcacacg agccgttcgc gtgcagtccc agatatatat agaggccgcc agggcctgcg 2581 gatcacacag gatctggagc tggtgctgat aacagcggaa tcccctgtct ggtcgagatc 2641 taagtaagct tggcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa 2701 aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc 2761 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt 2821 gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg 2881 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt 2941 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta 3001 taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc 3061 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat 3121 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac 3181 atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga 3241 caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc 3301 ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa 3361 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat 3421 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga 3481 agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc 3541 aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt 3601 acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa 3661 acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat 3721 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt 3781 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga 3841 attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa 3901 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga 3961 cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt 4021 ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt 4081 ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca 4141 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc 4201 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa 4261 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa 4321 attgtaaaat gtaactgtat tcagcgatga cgaaattctt agctattgta atactgcgat 4381 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt 4441 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc ggatctttgt 4501 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 4561 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 4621 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt 4681 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc 4741 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 4801 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg 4861 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa 4921 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct 4981 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt 5041 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 5101 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 5161 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 5221 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5281 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 5341 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 5401 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 5461 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 5521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 5581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 5641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 5701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 5761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 5821 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 5881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 5941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6061 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 6781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 7021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7381 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 7441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 7501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 7561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 7621 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 7681 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 7741 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 7801 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 7861 tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 7921 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg tggaggtttt 7981 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 8041 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 8101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 8161 caatgtatct tatggtactg taactgagct //