LOCUS Exported 10183 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Backbone for lentiviral stgRNA library construction (library 1 and 2). ACCESSION . VERSION . KEYWORDS Lenti_stgRNA-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10183) AUTHORS Park J, Lim JM, Jung I, Heo SJ, Park J, Chang Y, Kim HK, Jung D, Yu JH, Min S, Yoon S, Cho SR, Park T, Kim HH TITLE Recording of elapsed time and temporal information about biological events using Cas9. JOURNAL Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014. PUBMED 33539780 REFERENCE 2 (bases 1 to 10183) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10183 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..181 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 228..353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 1486..1726 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 1486..1506 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 1657..1676 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_feature 3747..3864 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 4003..5181 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 4234..5172 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 5129..5149 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 5194..5793 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5194..5213) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5690..5710 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5809..6397 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5862..5882) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6280..6291) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6469..6702 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6780..6901 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6817..6836) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6871..6890 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6941..7076 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7003..7022 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7096..7115) /label=T7 /note="T7 promoter, forward primer" promoter complement(7097..7115) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7125..7142) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7125..7141) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7134..7156) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7283..7738 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7370..7389) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7580..7601 /label=F1ori-F /note="F1 origin, forward primer" promoter 7764..7868 /gene="bla" /label=AmpR promoter CDS 7869..8729 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8087..8106) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8900..9488 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9389..9408 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9642..9659 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9776..9797 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9812..9842 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9850..9866 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9855..9877 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9874..9890 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9874..9890 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9909..9929 /label=T3 /note="T3 promoter, forward primer" promoter 9911..9929 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 9957..10183 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" ORIGIN 1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc 1501 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat 1561 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt 1621 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact 1681 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac 1741 ggttgtaaat gagcacacaa aatacacatg ctaaaatatt atattctatg acctttataa 1801 aatcaaccaa aatcttcttt ttaataactt tagtatcaat aattagaatt tttatgttcc 1861 tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc 1921 gttgttttct tcacctttaa taatagctac tccaccactt gttcctaagc ggtcagctcc 1981 tgcttcaatc attttttgag catcttcaaa tgttctaact ccaccagctg ctttaactaa 2041 agcattgtct ttaacaactg acttcattag tttaacatct tcaaatgttg cacctgattt 2101 tgaaaatcct gttgatgttt taacaaattc taatccagct tcaacagcta tttcacaagc 2161 tttcatgatt tcttcttttg ttaataaaca attttccata atacatttaa caacatgtga 2221 tccagctgct ttttttacag ctttcatgtc ttctaaaact aattcataat ttttgtcttt 2281 taatgcacca atatttaata ccatatcaat ttctgttgca ccatctttaa ttgcttcaga 2341 aacttcgaat gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta caacatttgt 2401 tattcctaca tttgtgcctt ttaataattc tttacaatag cttgttcaat atgaattaac 2461 acaaactgtt gcaaaatcaa attcaattgc ttcatcacat aattgtttaa tttcagcttt 2521 cgtagcatct tgttttaata atgtgtgatc tatatatttg tttagtttca ttttttctcc 2581 tatatattca tttttaattt taattcttta ataatttcgt ctactttaac tttagcgttt 2641 tgaacagatt caccaacacc tataaaataa atttttagtt taggttcagt tccacttggg 2701 cgaacagcaa atcatgactt atcttctaaa taaaatttta gtaagtcttg tcctggcata 2761 ttatacattc catcgatgta gtcttcaaca ttaacaactt taagtccagc aatttgagtt 2821 aagggtgttg ctctcaatga tttcattaat ggttcaattt ttaatttctt ttcttctggt 2881 ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca tttctttaaa taaatcttct 2941 aaatagtcta ctaatgtttt attttgtttt ttataaaatc aagcagcctc tgctattaat 3001 atagaagctt gtattccatc tttatctcta gctgagtcat caattacata tccataactt 3061 tcttcataag caaaaacaaa atttaatccg ttatcttctt ctttagcaat ttctctaccc 3121 attcatttaa atccagttaa agtttttaca atattaactc catatttttc atgagcgatt 3181 ctatcaccca aatcacttgt tacaaaactt gaatatagag ccggattttt tggaatgcta 3241 tttaagcgtt ttagatttga taattttcaa tcaattaaaa ttggtcctgt ttgatttcca 3301 tctaatctta caaaatgacc atcatgtttt attgccattc caaatctgtc agcatctggg 3361 tcattcataa taataatatc tgcatcatgt ttaataccat attcaagcgg tatttttcat 3421 gcaggatcaa attctggatt tggatttaca acatttttaa atgtttcatc ttcaaatgca 3481 tgctcttcaa cctcaataac gttatatcct gattcacgta atatttttgg ggtaaattta 3541 gttcctgttc cattaactgc gctaaaaata atttttaaat cttttttagc ttcttgctct 3601 tttttgtacg tctctgggtt agagctagaa atagcaagtt aacctaaggc tagtccgtta 3661 tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg 3721 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt 3781 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa 3841 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg 3901 cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt 3961 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag 4021 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4081 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4141 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4201 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg 4261 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag 4321 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc 4381 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc 4441 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc 4501 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag ttttgtaaat 4561 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc 4621 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc 4681 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt 4741 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga 4801 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag 4861 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca 4921 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg 4981 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 5041 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 5101 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 5161 cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc cacggtgcgc 5221 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5281 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5341 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5401 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5461 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5521 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga 5581 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5641 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5701 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5761 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg 5821 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5881 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5941 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6001 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6061 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6121 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6181 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 6241 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6301 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 6361 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt 6421 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 6481 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 6541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 6601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 6661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat 6721 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6781 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6841 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6901 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 6961 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 7021 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 7081 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 7141 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 7201 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 7261 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 7321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 7381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 7441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 7501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 7561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 7621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 7681 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 7741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 7801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 7861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 7921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 7981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 8041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 8101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 8161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 8221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 8281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 8341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 8401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 8461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 8521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 8581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 8641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 8701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 8761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 8821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 8881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 8941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 9001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 9061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 9121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 9181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 9241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 9301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 9361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 9421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 9481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 9541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 9601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 9661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 9721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 9781 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 9841 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 9901 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta 9961 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt 10021 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc 10081 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca 10141 ttgcagagat attgtattta agtgcctagc tcgatacata aac //