LOCUS       Exported               12226 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral vector for constitutive expression of FNLS in mammalian 
            cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-FNLS-P2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      232..241
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             241..306
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             313..333
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             358..1044
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1093..5193
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5206..5454
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5467..5487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5503..5559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5560..6159
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5560..5579)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6056..6076
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6175..6763
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6228..6248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6646..6657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6835..7068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7146..7267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7183..7202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7237..7256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7307..7442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7369..7388
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7462..7481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7463..7481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7491..7508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7491..7507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7649..8104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7736..7755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7946..7967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8130..8234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8235..9095
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8453..8472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9266..9854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9755..9774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10008..10025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10142..10163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10178..10208
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10216..10232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10221..10243
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10240..10256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10240..10256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10275..10295
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10277..10295
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10323..10549
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10550..10730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10777..10902
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11395..11628
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11813..11857
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12041..12158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg
      241 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
      301 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccatg
      361 agctcagaga ctggcccagt ggctgtggac cccacattga gacggcggat cgagccccat
      421 gagtttgagg tattcttcga tccgagagag ctccgcaagg agacctgcct gctttacgaa
      481 attaattggg ggggccggca ctccatttgg cgacatacat cacagaacac taacaagcac
      541 gtcgaagtca acttcatcga gaagttcacg acagaaagat atttctgtcc gaacacaagg
      601 tgcagcatta cctggtttct cagctggagc ccatgcggcg aatgtagtag ggccatcact
      661 gaattcctgt caaggtatcc ccacgtcact ctgtttattt acatcgcaag gctgtaccac
      721 cacgctgacc cccgcaatcg acaaggcctg cgggatttga tctcttcagg tgtgactatc
      781 caaattatga ctgagcagga gtcaggatac tgctggagaa actttgtgaa ttatagcccg
      841 agtaatgaag cccactggcc taggtatccc catctgtggg tacgactgta cgttcttgaa
      901 ctgtactgca tcatactggg cctgcctcct tgtctcaaca ttctgagaag gaagcagcca
      961 cagctgacat tctttaccat cgctcttcag tcttgtcatt accagcgact gcccccacac
     1021 attctctggg ccaccgggtt gaaaagcggc agcgagactc ccgggacctc agagtccgcc
     1081 acacccgaaa gtgacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     1141 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     1201 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     1261 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     1321 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     1381 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     1441 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     1501 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     1561 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     1621 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     1681 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     1741 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     1801 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     1861 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     1921 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     1981 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     2041 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     2101 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     2161 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     2221 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     2281 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     2341 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     2401 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     2461 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     2521 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     2581 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     2641 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     2701 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     2761 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     2821 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     2881 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     2941 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     3001 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     3061 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     3121 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     3181 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     3241 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     3301 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     3361 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     3421 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     3481 gaacacccag tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     3541 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     3601 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     3661 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     3721 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     3781 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     3841 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     3901 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     3961 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     4021 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     4081 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     4141 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     4201 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     4261 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     4321 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     4381 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     4441 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     4501 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     4561 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     4621 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     4681 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     4741 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     4801 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     4861 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     4921 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     4981 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     5041 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     5101 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     5161 acacggatcg acctgtctca gctgggaggc gattcaggcg gatctactaa tctgtcagat
     5221 attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct catgctccca
     5281 gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt gcacaccgcc
     5341 tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc tgaatacaag
     5401 ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat gctctctggt
     5461 ggttctccca agaagaagag gaaagtcgct agcggcagcg gcgccaccaa cttcagcctg
     5521 ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta caagcccacg
     5581 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5641 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5701 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5761 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5821 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5881 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5941 gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6001 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6061 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6121 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa
     6181 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6241 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6301 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6361 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6421 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6481 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6541 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6601 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6661 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6721 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     6781 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6841 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     7081 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7141 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7201 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7261 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7381 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7441 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7501 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7561 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7621 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7861 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7981 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8101 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8221 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8281 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8341 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8401 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8461 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8521 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8581 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8641 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8701 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8761 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8821 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8881 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8941 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9001 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9061 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9961 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10021 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10081 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10141 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10201 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10261 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
    10321 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
    10381 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
    10441 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
    10501 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
    10561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10741 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10801 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10861 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10921 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10981 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    11041 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    11101 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    11161 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    11221 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    11281 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    11341 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    11401 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    11461 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11521 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11581 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11641 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11701 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11761 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11821 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11881 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11941 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    12001 tcagacccac ctcccaaccc cgaggggacc cagaccacaa ttttaaaaga aaagggggga
    12061 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    12121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    12181 gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagt
//
