LOCUS       Exported                7320 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  AAV plasmid expressing TGFB3 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-hRedOpsin-TGFB3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Wang SK, Xue Y, Cepko CL
  TITLE     Microglia modulation by TGF-beta1 protects cones in mouse models of 
            retinal degeneration.
  JOURNAL   J Clin Invest. 2020 Aug 3;130(8):4360-4369. doi: 10.1172/JCI136160.
  PUBMED    32352930
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(3570..3590)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3988..3999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4066..4083)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4072..4279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4367..4496
                     /label=AAV2 ITR
     primer_bind     complement(4515..4532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4515..4531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4524..4546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4673..5128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4760..4779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4970..4991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5154..5258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5259..6119
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5477..5496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6290..6878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7032..7049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7166..7187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7202..7232
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7240..7256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7245..7267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7264..7280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7264..7280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7318..127
                     /label=AAV2 ITR
ORIGIN
        1 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgkgc gacctttggy
       61 cgsccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      121 ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg
      181 aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta tgaggctgag
      241 ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga gcccggcagc
      301 tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag gtaggaaggt
      361 cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat ccaatggttg
      421 gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg cacagaaggc
      481 catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa gggtgggtgc
      541 aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc ttccgtttca
      601 tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt acacacggta
      661 gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt gtcaaaggca
      721 aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta gtgacaaagc
      781 ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg cgtggagtca
      841 tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct ggccagggcc
      901 ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac attggggccc
      961 ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg ggctttcttg
     1021 ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct cgttcttcat
     1081 atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt gggaccacaa
     1141 atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt gactttctag
     1201 gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct aatcatcaat
     1261 tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc cacctctgcc
     1321 acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag ggcattggtg
     1381 ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag ggcgagcgga
     1441 gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc actcctcctc
     1501 tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa atagcatggt
     1561 ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca gaaggggaat
     1621 ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca tggcggtaat
     1681 gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca ggtgatctca
     1741 gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc ggccctaaat
     1801 agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct ctgagcagtt
     1861 tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc ccaaaggcgg
     1921 acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca cagcggctcc
     1981 cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg gttcccagca
     2041 aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag aggtgggagg
     2101 aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt gggagctttt
     2161 aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga ccctcaggtg
     2221 atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc catgaagatg
     2281 cacttgcaaa gggctctggt agtcctggcc ctgctgaact tggccacaat cagcctctct
     2341 ctgtccactt gcaccacgtt ggacttcggc cacatcaaga agaagagggt ggaagccatt
     2401 aggggacaga tcttgagcaa gctcaggctc accagccccc ctgagccatc ggtgatgacc
     2461 cacgtcccct atcaggtcct ggcactttac aacagcaccc gggagttgct ggaagagatg
     2521 cacggggaga gggaggaagg ctgcactcag gagacctcgg agtctgagta ctatgccaaa
     2581 gagatccata aattcgacat gatccaggga ctggcggagc acaatgaact ggccgtctgc
     2641 cccaaaggaa ttacctctaa ggtttttcgt ttcaatgtgt cctcagtgga gaaaaatgga
     2701 accaatctgt tccgggcaga gttccgggtc ttgcgggtgc ccaaccccag ctccaagcgc
     2761 acagagcaga gaattgagct cttccagata cttcgaccgg atgagcacat agccaagcag
     2821 cgctacatag gtggcaagaa tctgcccaca aggggcaccg ctgaatggct gtctttcgat
     2881 gtcactgaca ctgtgcgcga gtggctgttg aggagagagt ccaacttggg tctggaaatc
     2941 agcatccact gtccatgtca cacctttcag cccaatggag acatactgga aaatgttcat
     3001 gaggtgatgg aaatcaaatt caaaggagtg gacaatgaag atgaccatgg ccgtggagac
     3061 ctggggcgtc tcaagaagca aaaggatcac cacaacccac acctgatcct catgatgatc
     3121 cccccacacc gactggacag cccaggccag ggcagtcaga ggaagaagag ggccctggac
     3181 accaattact gcttccgcaa cctggaggag aactgctgtg tacgccccct ttatattgac
     3241 ttccggcagg atctaggctg gaaatgggtc cacgaaccta agggttacta tgccaacttc
     3301 tgctcaggcc cttgcccata cctccgcagc gcagacacaa cccatagcac ggtgcttgga
     3361 ctatacaaca ccctgaaccc agaggcgtct gcctcgccat gctgcgtccc ccaggacctg
     3421 gagcccctga ccatcttgta ctatgtgggc agaaccccca aggtggagca gctgtccaac
     3481 atggtggtga agtcgtgtaa gtgcagctga accggtaatc aacctctgga ttacaaaatt
     3541 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3601 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3661 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3721 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3781 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3841 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3901 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3961 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4021 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgag atctgcctcg actgtgcctt
     4081 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4141 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4201 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4261 atagcaggca tgctggggac tcgagttaag ggcgaattcc cgataaggat cttcctagag
     4321 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     4381 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4441 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt
     4501 aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4741 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4861 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4981 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5041 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5101 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7141 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7201 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7261 acacaggaaa cagctatgac catgattacg ccagatttaa ttaaggcctt aattaggctg
//
