LOCUS Exported 9895 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive lentiviral expression of human TMPRSS2.. ACCESSION . VERSION . KEYWORDS pLEX307-TMPRSS2-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9895) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 9895) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9895 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1318 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2804..2828) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2836..2877 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2928..3516 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2981..3001) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3399..3410) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3588..3821 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3899..4020 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3936..3955) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3990..4009 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4060..4195 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4215..4234) /label=T7 /note="T7 promoter, forward primer" promoter complement(4216..4234) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4244..4261) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4244..4260) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4253..4275) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4402..4857 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4489..4508) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4699..4720 /label=F1ori-F /note="F1 origin, forward primer" promoter 4883..4987 /gene="bla" /label=AmpR promoter CDS 4988..5848 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5206..5225) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6019..6607 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6508..6527 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6761..6778 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6895..6916 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6931..6961 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6969..6985 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6974..6996 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6993..7009 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6993..7009 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7028..7048 /label=T3 /note="T3 promoter, forward primer" promoter 7030..7048 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7076..7302 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7303..7483 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7530..7655 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8148..8381 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8566..8610 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8841..9051 /label=SV40 promoter /note="SV40 early promoter" promoter 8863..9059 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8910..9045 /label=SV40 ori /note="SV40 origin of replication" CDS 9091..9693 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(9091..9110) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9587..9607 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9736..9853 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac 1321 aaaatggctt tgaactcagg gtcaccacca gctattggac cttactatga aaaccatgga 1381 taccaaccgg aaaaccccta tcccgcacag cccactgtgg tccccactgt ctacgaggtg 1441 catccggctc agtactaccc gtcccccgtg ccccagtacg ccccgagggt cctgacgcag 1501 gcttccaacc ccgtcgtctg cacgcagccc aaatccccat ccgggacagt gtgcacctca 1561 aagactaaga aagcactgtg catcaccttg accctgggga ccttcctcgt gggagctgcg 1621 ctggccgctg gcctactctg gaagttcatg ggcagcaagt gctccaactc tgggatagag 1681 tgcgactcct caggtacctg catcaacccc tctaactggt gtgatggcgt gtcacactgc 1741 cccggcgggg aggacgagaa tcggtgtgtt cgcctctacg gaccaaactt catccttcag 1801 gtgtactcat ctcagaggaa gtcctggcac cctgtgtgcc aagacgactg gaacgagaac 1861 tacgggcggg cggcctgcag ggacatgggc tataagaata atttttactc tagccaagga 1921 atagtggatg acagcggatc caccagcttt atgaaactga acacaagtgc cggcaatgtc 1981 gatatctata aaaaactgta ccacagtgat gcctgttctt caaaagcagt ggtttcttta 2041 cgctgtatag cctgcggggt caacttgaac tcaagccgcc agagcaggat cgtgggcggc 2101 gagagcgcgc tcccgggggc ctggccctgg caggtcagcc tgcacgtcca gaacgtccac 2161 gtgtgcggag gctccatcat cacccccgag tggatcgtga cagccgccca ctgcgtggaa 2221 aaacctctta acaatccatg gcattggacg gcatttgcgg ggattttgag acaatctttc 2281 atgttctatg gagccggata ccaagtagaa aaagtgattt ctcatccaaa ttatgactcc 2341 aagaccaaga acaatgacat tgcgctgatg aagctgcaga agcctctgac tttcaacgac 2401 ctagtgaaac cagtgtgtct gcccaaccca ggcatgatgc tgcagccaga acagctctgc 2461 tggatttccg ggtggggggc caccgaggag aaagggaaga cctcagaagt gctgaacgct 2521 gccaaggtgc ttctcattga gacacagaga tgcaacagca gatatgtcta tgacaacctg 2581 atcacaccag ccatgatctg tgccggcttc ctgcagggga acgtcgattc ttgccagggt 2641 gacagtggag ggcctctggt cacttcgaag aacaatatct ggtggctgat aggggataca 2701 agctggggtt ctggctgtgc caaagcttac agaccaggag tgtacgggaa tgtgatggta 2761 ttcacggact ggatttatcg acaaatgagg gcagacggct aaaacccagc tttcttgtac 2821 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 2881 taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg 2941 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 3001 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 3061 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 3121 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 3181 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 3241 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 3301 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca 3361 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 3421 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 3481 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta 3541 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 3601 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 3661 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 3721 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 3781 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg 3841 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4021 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc 4081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 4141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg 4201 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac 4261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 4321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 4381 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 4441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 4741 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 4801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 4861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 4921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 4981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 5041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 5101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 5161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 5221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 5281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 5341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 5401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 5461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 5521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 5581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 5641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 5701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 5761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 5821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 5881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 5941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 6001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 6061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 6121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 6181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 6241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 6301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 6481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 6541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 6601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 6661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 6721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 6781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 6841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 6901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 6961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 7021 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag 7081 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta 7141 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc 7201 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat 7261 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac 7321 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 7381 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 7441 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg 7501 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag 7561 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 7621 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 7681 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata 7741 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat 7801 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag 7861 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga 7921 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca 7981 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg 8041 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc 8101 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc 8161 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta 8221 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt 8281 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga 8341 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct 8401 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg 8461 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca 8521 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa 8581 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg 8641 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt 8701 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc 8761 cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg 8821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag 8881 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 8941 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 9001 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 9061 agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc 9121 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 9181 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 9241 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 9301 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 9361 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 9421 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 9481 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 9541 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 9601 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 9661 tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc 9721 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag 9781 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa 9841 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccgg //