LOCUS Exported 3053 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter. ACCESSION . VERSION . KEYWORDS DT039A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3053) AUTHORS Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS TITLE Artificial cells drive neural differentiation. JOURNAL Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep. PUBMED 32948587 REFERENCE 2 (bases 1 to 3053) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3053 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 289..308 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(527..631) /gene="bla" /label=AmpR promoter primer_bind 699..717 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 721..764 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 767..786 /label=BioBrick prefix /note="BioBrick prefix for parts that start with ""ATG""" CDS 851..874 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 875..1588 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" terminator 1714..1761 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 1763..1783 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1789..1806) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1960..2548) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2040..2059) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2719..526) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 61 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 661 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 721 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 781 ttctagattg acagctagct cagtcctagg gattgtgcta gcgatactag aagaaggaga 841 ataatctatg gactacaaag acgatgatga caagatgcgt aaaggcgaag agctgttcac 901 tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt 961 gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac 1021 tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca 1081 gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc 1141 ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg 1201 tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga 1261 ctttaaagaa gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa 1321 tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca 1381 caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg 1441 tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa 1501 agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat 1561 cacgcatggt atggatgaac tgtacaaata ataacgactc aggctgctac gcctgtgtac 1621 tggaaaacaa aaccaaaacc caaaaaacaa aaaactgagc ccattggtat cgtggaagga 1681 ctcgatcaaa aaaaaaaaaa aaaaaaaaaa aaactagcat aaccccttgg ggcctctaaa 1741 cgggtcttga ggggtttttt ggtactagta gcggccgctg caggcttcct cgctcactga 1801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 1861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 1921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc 1981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 2101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 2161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 2221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 2281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 2341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 2401 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 2461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 2521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 2581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 2641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 2701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 2761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 2821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 2881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 2941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cac //