LOCUS Exported 2703 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Constitutive murine mature BDNF expression, controlled under E.coli tac promoter. ACCESSION . VERSION . KEYWORDS DT033A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2703) AUTHORS Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS TITLE Artificial cells drive neural differentiation. JOURNAL Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep. PUBMED 32948587 REFERENCE 2 (bases 1 to 2703) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2703 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 273..292 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(511..615) /gene="bla" /label=AmpR promoter primer_bind 683..701 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 705..748 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 751..772 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" promoter 773..801 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" CDS 835..858 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" terminator 1348..1395 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 1397..1417 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1423..1440) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1594..2182) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1674..1693) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2353..510) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agttgacaat 781 taatcatcgg ctcgtataat gtgtgggata ctagaagaag gagaataatc tatggactac 841 aaagacgatg atgacaagat gcactccgac cctgcccgcc gtggggagct gagcgtgtgt 901 gacagtatta gcgagtgggt cacagcggca gataaaaaga ctgcagtgga catgtctggc 961 gggacggtca cagtcctaga gaaagtcccg gtatccaaag gccaactgaa gcagtatttc 1021 tacgagacca agtgtaatcc catgggttac accaaggaag gctgcagggg catagacaaa 1081 aggcactgga actcgcaatg ccgaactacc caatcgtatg ttcgggccct tactatggat 1141 agcaaaaaga gaattggctg gcgattcata aggatagaca cttcctgtgt atgtacactg 1201 accattaaga ggggaagata gtagcgactc aggctgctac gcctgtgtac tggaaaacaa 1261 aaccaaaacc caaaaaacaa aaaactgagc ccattggtat cgtggaagga ctcgatcaaa 1321 aaaaaaaaaa aaaaaaaaaa aaaaaaacta gcataacccc ttggggcctc taaacgggtc 1381 ttgaggggtt ttttggtact agtagcggcc gctgcaggct tcctcgctca ctgactcgct 1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 2701 tat //