LOCUS Exported 2175 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPKK0CM0063 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2175) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2175) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2175 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(11..599) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(91..110) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 782..876 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(897..1556) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1471..1490 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 1718..1736 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1740..1783 /label=bacterial terminator /note="putative bacterial transcription terminator" protein_bind 1811..1844 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" protein_bind 1870..1903 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" terminator 1934..1991 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(2015..2032) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 61 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 421 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 721 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg 781 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga 841 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac 901 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg 961 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct 1021 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg 1081 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca 1141 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat 1201 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca 1261 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg 1321 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata 1381 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc 1441 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct 1501 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta 1561 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt 1621 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt 1681 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa 1741 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cggtctcagg 1801 agcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc actccccgag 1861 ccggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca ctcccctgac 1921 cgagaccctg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa 1981 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg 2041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 2161 gtaaaaaggc cgcgt //