LOCUS Exported 2303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPKK0CM0111 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2303) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2303 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 523..617 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(638..1297) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1212..1231 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 1459..1477 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1481..1524 /label=bacterial terminator /note="putative bacterial transcription terminator" protein_bind 1556..1589 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" protein_bind 1615..1648 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" promoter 1665..1711 /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" terminator 1803..1860 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1884..1901) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2055..340) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2135..2154) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 61 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 481 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa 541 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg 601 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat 661 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 721 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 781 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 841 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 901 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 961 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 1021 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa 1081 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 1141 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 1201 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 1261 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 1321 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 1381 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc 1441 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc 1501 ttagctttcg ctaaggatga tttctggaat tcggtctcac catggtgcgg tgcgggtgcc 1561 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca 1621 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cttcgcaaga cccttcctct 1681 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa 1741 caaaacaaac aacaaacaac attacaattt actattctag tcgaaaatgc gagaccctgc 1801 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga 1861 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 1981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 2041 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc 2101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 2161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 2221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 2281 taggtcgttc gctccaagct ggg //