LOCUS Exported 845 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pcDNA3.2/V5 mmu-mir-122 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 845) AUTHORS Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP TITLE Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. JOURNAL Genes Dev. 2010 May 15. 24(10):992-1009. PUBMED 20413612 REFERENCE 2 (bases 1 to 845) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..845 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 33..52 /label=T7 /note="T7 promoter, forward primer" promoter 33..51 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 81..105 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(409..433) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 459..500 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(540..559) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 584..632 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 61 gctggctagt taagctatca acaagtttgt acaaaaaagc aggctcccta tgctccagtt 121 tcagcgtttg gaaccactga ggagtctgtg acagacaacg gaggaatgtg aagggaaggt 181 taataccctt gtgcctgaca gactttcctt agcagagctg tggagtgtga caatggtgtt 241 tgtgtccaaa ccatcaaacg ccattatcac actaaatagc tactgctagg caatccgtcc 301 actccacgcg tgacttgacg tctgttctct ccgagcaaga agttctgtct tgtgctagag 361 ttctccattg tgtgtaatgt atgaataaaa tctggtcctc ttgtgcttac ccagctttct 421 tgtacaaagt ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc 481 tcctcggtct cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact 541 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 601 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 661 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 721 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 781 ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc 841 ctgta //