LOCUS Exported 10709 bp ds-DNA circular SYN 08-MAR-2023 DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259). ACCESSION . VERSION . KEYWORDS psPAX2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10709) TITLE Trono Lab Packaging and Envelope Plasmids REFERENCE 2 (bases 1 to 10709) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 8, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10709 /organism="synthetic DNA construct" /mol_type="other DNA" intron 654..1670 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1594..1616 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1678..1697 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 1776..3278 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS 3071..6082 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 5767..5884 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 6656..6889 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7074..7118 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" CDS 7412..7438 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" primer_bind complement(7723..7742) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 7788..7843 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7842..7861) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(8204..8220) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8204..8220) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8217..8239) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8228..8244 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8252..8282) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8297..8318 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8377..8572 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8423..8558 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8485..8504 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 8578..8712 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8615..8634) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8669..8688 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8780..8797) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8951..9539) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9031..9050) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9710..10570) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10333..10352 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10571..10675) /gene="bla" /label=AmpR promoter enhancer 10706..376 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 361 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc 421 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg 481 ggcggggggg gggggggggc gcgccrgggg gggggggggg gggggggggg gggggggggg 541 sgrggsggrg rggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta 601 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg 661 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc 721 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct 781 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg 841 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg 901 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc 961 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg 1021 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 1081 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc 1141 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg 1201 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc 1261 cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc 1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 1381 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 1441 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 1501 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg cgttgacgcg 1741 cacggcaaga ggcgaggggc ggcgactggt gagagatggg tgcgagagcg tcagtattaa 1801 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 2101 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca 2161 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac 2221 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga 2281 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa 2341 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag 2401 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga 2461 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat 2521 ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg 2581 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac 2641 caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag 2701 cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag 2761 attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag 2821 catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa gcaatgagcc 2881 aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac caaagaaaga 2941 ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta 3001 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgtactgaga 3061 gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca gggaattttc 3121 ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt ggggaagaga 3181 caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct ttagcttccc 3241 tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc aattaaagga 3301 agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt tgccaggaag 3361 atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaagac agtatgatca 3421 gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag gacctacacc 3481 tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa attttcccat 3541 tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc caaaagttaa 3601 acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta cagaaatgga 3661 aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc cagtatttgc 3721 cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag aacttaataa 3781 gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag ggttaaaaca 3841 gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc ccttagataa 3901 agacttcagg aagtatactg catttaccat acctagtata aacaatgaga caccagggat 3961 tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa tattccagtg 4021 tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag tcatctatca 4081 atacatggat gatttgtatg taggatctga cttagaaata gggcagcata gaacaaaaat 4141 agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca aaaaacatca 4201 gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat ggacagtaca 4261 gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga aattagtggg 4321 aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat tatgtaaact 4381 tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag cagagctaga 4441 actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt atgacccatc 4501 aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat atcaaattta 4561 tcaagagcca tttaaaaatc tgaaaacagg aaagtatgca agaatgaagg gtgcccacac 4621 taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa gcatagtaat 4681 atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg aagcatggtg 4741 gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata cccctccctt 4801 agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa ctttctatgt 4861 agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa ctgacagagg 4921 aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt tacaagcaat 4981 tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact cacaatatgc 5041 attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca gtcaaataat 5101 agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac acaaaggaat 5161 tggaggaaat gaacaagtag ataaattggt cagtgctgga atcaggaaag tactattttt 5221 agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt ggagagcaat 5281 ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca gctgtgataa 5341 atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag gaatatggca 5401 gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg tagccagtgg 5461 atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat acttcctctt 5521 aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca gcaatttcac 5581 cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat ttggcattcc 5641 ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa agaaaattat 5701 aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg cagtattcat 5761 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 5821 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa 5881 ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag caaagctcct 5941 ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag tagtgccaag 6001 aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg attgtgtggc 6061 aagtagacag gatgaggatt aacacatgga attctgcaac aactgctgtt tatccatttc 6121 agaattgggt gtcgacatag cagaataggc gttactcgac agaggagagc aagaaatgga 6181 gccagtagat cctagactag agccctggaa gcatccagga agtcagccta aaactgcttg 6241 taccaattgc tattgtaaaa agtgttgctt tcattgccaa gtttgtttca tgacaaaagc 6301 cttaggcatc tcctatggca ggaagaagcg gagacagcga cgaagagctc atcagaacag 6361 tcagactcat caagcttctc tatcaaagca gtaagtagta catgtaatgc aacctataat 6421 agtagcaata gtagcattag tagtagcaat aataatagca atagttgtgt ggtccatagt 6481 aatcatagaa tataggaaaa tggccgctga tcttcagacc tggaggagga gatatgaggg 6541 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 6601 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 6661 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 6721 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 6781 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 6841 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 6901 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 6961 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 7021 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 7081 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 7141 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 7201 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 7261 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 7321 gtggagagag agacagagac agatccattc gattagtgaa cggatccttg gcacttatct 7381 gggacgatct gcggagcctg tgcctcttca gctaccaccg cttgagagac ttactcttga 7441 ttgtaacgag gattgtggaa cttctgggac gcagggggtg ggaagccctc aaatattggt 7501 ggaatctcct acaatattgg agtcaggagc taaagaatag tgctgttagc ttgctcaatg 7561 ccacagccat agcagtagct gaggggacag atagggttat agaagtagta caaggagctt 7621 gtagagctat tcgccacata cctagaagaa taagacaggg cttggaaagg attttgctat 7681 aagctcgaaa caaccggtac ctctagaact atagctagca gatctttttc cctctgccaa 7741 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 7801 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 7861 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 7921 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 7981 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 8041 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 8101 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 8161 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 8221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 8281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 8341 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 8401 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 8461 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 8521 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 8581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 8641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 8701 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 8761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 8821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 8881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 8941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 9001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 9061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 9121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 9181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 9241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 9301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 9361 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 9421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 9481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 9541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 9841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 9901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 9961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 10021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 10081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 10141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 10201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 10261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 10321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 10381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 10441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 10501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 10561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 10621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 10681 ttccccgaaa agtgccacct gggtcgaca //