LOCUS Exported 4331 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV-myc-Atg12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4331) AUTHORS Lee IH, Finkel T TITLE Regulation of autophagy by the p300 acetyltransferase. JOURNAL J Biol Chem. 2009 Mar 6. 284(10):6322-8. PUBMED 19124466 REFERENCE 2 (bases 1 to 4331) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4331 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 27..330 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 331..534 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 485..505 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 531..555 /label=LNCX /note="Human CMV promoter, forward primer" intron 672..768 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" polyA_signal 1470..1604 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1494..1513) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1548..1567 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1715..1731) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1715..1731) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1728..1750) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1739..1755 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1763..1793) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1808..1829 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1946..1963) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2117..2705) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2197..2216) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2876..3736) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3499..3518 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3737..3841) /gene="bla" /label=AmpR promoter primer_bind 3909..3927 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(3965..3987) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4087..4106 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4300..4322 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4314..4331 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4315..4331 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg 841 cagaagctga tctcagagga ggacctgctt atggccatgg aggcccgaat tcgaatgact 901 agccgggaac accaagtttc actgtgtaat tgcgtccccc tactccggcg cctcctttgc 961 gacgctccct ggagaaaagc acgcccactg cacgcgctca gtcgctactt ccgctctcga 1021 gtgtctccaa gcaagatggc ggaggagccg cagtctgtgt tgcagcttcc tacttcaatt 1081 gctgctggag gggaaggact tacggatgtc tccccagaaa caaccacccc ggagcccccg 1141 tcttccgctg cagtttcccc gggaacagag gaacctgctg gcgacaccaa gaaaaaaatt 1201 gacattttgc taaaggctgt gggagacact cctattatga aaacaaagaa gtgggcagta 1261 gagcgaacac gaaccatcca aggactcatt gacttcatca aaaagtttct taaacttgtg 1321 gcctcagaac agttgtttat ttatgtgaat cagtcctttg ctccttcccc agaccaagaa 1381 gttggaactc tctatgagtg ttttggcagt gatggtaaac tggttttaca ttactgcaag 1441 tctcaggcgt ggggatgagc ggccgcgggg atccagacat gataagatac attgatgagt 1501 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 1561 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 1621 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt 1681 cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 1801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 1861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 1921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 2041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 2101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 2161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 2221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 2281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 2341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 2401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 2461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 2521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 2581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 2641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 2821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 2881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 3001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 3061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 3121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 3181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 3241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 3301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 3361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 3601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 3661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 3721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 3781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 3841 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 3901 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 3961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 4021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 4081 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 4141 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 4201 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 4261 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 4321 cgacggccag t //