LOCUS       Exported                 854 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLKO.1 Sox2 HM a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Bass AJ, Watanabe H, Mermel CH, Yu S, Perner S, Verhaak RG, Kim SY, 
            Wardwell L, Tamayo P, Gat-Viks I, Ramos AH, Woo MS, Weir BA, Getz G,
            Beroukhim R, O'Kelly M, Dutt A, Rozenblatt-Rosen O, Dziunycz P, 
            Komisarof J, Chirieac LR, Lafargue CJ, Scheble V, Wilbertz T, Ma C, 
            Rao S, Nakagawa H, Stairs DB, Lin L, Giordano TJ, Wagner P, Minna 
            JD, Gazdar AF, Zhu CQ, Brose MS, Cecconello I, Jr UR, Marie SK, Dahl
            O, Shivdasani RA, Tsao MS, Rubin MA, Wong KK, Regev A, Hahn WC, Beer
            DG, Rustgi AK, Meyerson M
  TITLE     SOX2 is an amplified lineage-survival oncogene in lung and 
            esophageal squamous cell carcinomas.
  JOURNAL   Nat Genet. 2009 Nov . 41(11):1238-42.
  PUBMED    19801978
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    125..242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        291..801
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     720..738
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(823..842)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atatatcttg tggaaaggac gaaacaccgg cgagataaac atggcaatca actcgagttg
       61 attgccatgt ttatctcgtt tttgaattct cgacctcgag acaaatggca gtattcatcc
      121 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      181 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      241 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
      301 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      361 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      421 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      481 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      541 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      601 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      661 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      721 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      781 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
      841 acggtgcgcc tcgc
//
