LOCUS Exported 3244 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.. ACCESSION . VERSION . KEYWORDS pME-mScarlet-NS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3244) TITLE New_for_revision JOURNAL Unpublished REFERENCE 2 (bases 1 to 3244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3244 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 217..234 /label=L4440 /note="L4440 vector, forward primer" terminator 393..420 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 512..598 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 647..669 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 661..678 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 662..678 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 694..793 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" regulatory 794..803 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(1494..1593) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1610..1629) /label=T7 /note="T7 promoter, forward primer" promoter complement(1611..1629) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1634..1650) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1634..1650) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1752..1771) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1763..2572 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1830..1849) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2719..63 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3208..3227 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 61 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 361 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 421 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 481 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 541 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 601 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 661 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 721 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac 781 aaaaaagcag gctgccacca tggtatctaa aggagaggct gtcatcaaag agtttatgag 841 atttaaagtc cacatggagg gaagcatgaa cggtcatgag tttgaaatag aaggagaagg 901 tgagggtcga ccttacgaag gtacccagac cgcgaagttg aaagttacca aagggggccc 961 cttgccgttc agttgggaca tcctgtcccc tcaatttatg tacggaagca gggcttttac 1021 gaaacatccg gctgacattc ccgattacta taagcaaagt ttccccgagg gctttaagtg 1081 ggagcgcgta atgaattttg aggatggcgg tgctgttaca gtcacacagg atacatccct 1141 cgaagacgga acgttgatct acaaagttaa acttcgcggg actaacttcc cgcccgatgg 1201 ccctgtgatg caaaagaaga ctatgggatg ggaggccagc acggaaagac tctaccctga 1261 agatggcgta cttaaaggtg atataaaaat ggctctgcgc ctgaaggatg gaggtcgtta 1321 cctcgccgat ttcaagacca cctacaaagc caaaaaaccc gtgcagatgc ctggcgcata 1381 taatgtagat aggaagctgg acatcactag ccataatgaa gattacaccg ttgtagagca 1441 atacgaacga tcagagggcc gtcactcaac cggcggtatg gatgaactct ataagccagc 1501 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca 1561 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga 1621 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg 1681 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 1741 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 1801 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 1861 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 1921 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 1981 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 2041 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 2101 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 2161 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 2221 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 2281 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 2341 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 2401 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 2461 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 2521 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 2581 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 2641 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga 2701 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 2761 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 2821 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 2881 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 2941 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 3001 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 3061 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 3121 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 3181 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 3241 gttt //