LOCUS       Exported                9766 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses Smo-FLAG K448R.
ACCESSION   .
VERSION     .
KEYWORDS    GP823
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      116..704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     605..624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     858..875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     967..986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    1115..1136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1151..1181
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1189..1205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1194..1216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1213..1229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1213..1229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1490..2123
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2170..2295
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2792..3025
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3210..3254
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3550..3667
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3716..3764
                     /note="synthetic polyadenylation signal"
     misc_feature    3778..3869
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3818..3837
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3884..4018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3921..3940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             4214..4609
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4610..5195
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4776..4793)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5003..5022
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7582..7591
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7591..7656
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7697..8285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7750..7770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8168..8179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8433..8613
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8660..8678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8746..8850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8851..9711
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9069..9088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
       61 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      901 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
      961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1021 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
     1081 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     1141 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     1201 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
     1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1321 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     1381 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     1441 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     1501 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     1561 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     1621 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     1681 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     1741 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     1801 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     1861 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     1921 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2101 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     2161 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     2221 gggcggcgac tgcagagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     2281 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     2341 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     2401 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     2461 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     2521 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     2581 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     2641 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     2701 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2761 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2821 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2881 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2941 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     3001 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     3061 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     3121 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     3181 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     3241 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3301 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     3361 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     3421 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     3481 ccattcgatt agtgaacgga tctcgacggt atcgccgaat tctcaacaat aggcagtatt
     3541 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     3601 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     3661 aaattttcgg gtttattact cggacagcag agatccagtt tggactagtg gccgcaataa
     3721 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     3781 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     3841 tgcaagtgca ggtgccagaa catttctcta tcgataggta ccgaacttgt ttattgcagc
     3901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccc
     4021 cgggaacaga ttcgcgacta gtggacacac aggatagggg tgggcagcat gaggggggcc
     4081 agagggagag ggggcagagc tgggctggac gaggggacac cccgccccaa tggggcgtga
     4141 cgagctgcca gcccaggctc cggggcacgt aaaagcgggg ctgtgagacc ggagagcacg
     4201 gagcgacatt aatatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     4261 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     4321 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     4381 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     4441 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     4501 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     4561 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc ccctctccct
     4621 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4681 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4741 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4801 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4861 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4921 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4981 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5041 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     5101 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     5161 cctttgaaaa acacgatgat aatatggcca caaccataga ccatggccgc tggccgcccc
     5221 gtgcgtgggc ccgagctggc gccccggagg ctgctgcagt tgctgctgct ggtactgctg
     5281 gggggcccgg gccggggggc ggccttgagc gggaacgtga ccgggcctgg gcctcacagc
     5341 gccagcggga gctcgaggag ggacgtgccg gtgaccagcc ctccgccgcc gctgctgagc
     5401 cactgcggcc gggccgccca ctgcgagcct ttgcgctaca acgtgtgcct gggctcggcg
     5461 ctgccctacg gagccaccac cacgctgctg gctggggact cggactcgca ggaggaagcg
     5521 cacggcaagc tcgtgctctg gtccggcctc cggaatgccc cccgctgctg ggcagtgatc
     5581 cagcccctgc tgtgtgctgt ctacatgccc aagtgtgaga atgaccgagt ggagttgccc
     5641 agccgtaccc tctgccaggc cacccgaggc ccctgtgcca ttgtggagcg ggagcgaggg
     5701 tggcctgact ttctgcgttg cacaccggac cacttccctg aaggctgccc aaacgaggta
     5761 caaaacatca agttcaacag ctcaggccaa tgtgaagcac ccttggtgcg aacagacaac
     5821 cccaagagct ggtatgagga cgtggagggc tgtgggattc agtgtcagaa cccgctgttc
     5881 accgaggccg agcaccagga catgcacagc tacatcgcag ccttcggggc ggtcactggt
     5941 ctctgcacgc tcttcacact ggccaccttt gtggctgact ggcggaactc caatcgctac
     6001 cctgcggtta ttctcttcta tgtcaatgcg tgtttcttcg tgggcagcat tggctggctg
     6061 gcccagttca tggatggtgc ccgccgagag attgtttgcc gagcagatgg caccatgaga
     6121 tttggggagc ccacctccag tgagaccctg tcctgtgtca tcatctttgt cattgtgtac
     6181 tatgccttga tggctggagt agtctggttc gtggtcctca cctatgcctg gcacacctcc
     6241 ttcaaagccc tgggcaccac ctaccagcct ctctcgggca agacatccta tttccacctg
     6301 ctcacgtggt cactcccctt tgtcctcacg gtggcaatcc tggctgtggc tcaggtagat
     6361 ggagactccg tgagtggcat ctgttttgta ggctacaaga actatcggta ccgtgctggc
     6421 tttgtcctgg ccccaattgg cctggtgctt attgtgggag gctacttcct catcagaggg
     6481 gtcatgactc tgttctccat caagagcaac caccctgggc ttctgagtga gaaggcagcc
     6541 agcaggatca acgagaccat gctgcgcctg ggcatttttg gcttcctggc ctttggcttt
     6601 gtgctcatca ccttcagctg ccacttctat gacttcttca accaggctga gtgggagcgt
     6661 agcttccggg actatgtgct atgccaagcc aacgtgacca tcgggctgcc taccaagaag
     6721 cccattcctg actgtgagat caagaatcgg cccagcctcc tggtggagaa gatcaatcta
     6781 tttgccatgt ttggcactgg cattgccatg agcacctggg tctggaccaa ggccaccctg
     6841 ctcatctgga ggcgcacctg gtgcaggttg actgggcaca gtgatgatga gcccaagaga
     6901 atcaagaaga gcaagatgat cgccaaggcc ttctctaagc ggcgtgagct gctgcagaac
     6961 ccgggccagg agctctcctt cagcatgcac actgtctccc atgatggacc tgttgcgggt
     7021 ttggcttttg acctcaatga accctcagct gacgtctcct ctgcctgggc tcagcatgtc
     7081 accaagatgg tggctcggag aggagccata ttgccccagg atgtgtccgt tacccctgtg
     7141 gcaactccag tgccaccaga agaacaagcc aacatgtggc tggttgaggc agagatctcc
     7201 ccagagttag agaagcgttt gggccggaag aaaaagcgga ggaagaggaa gaaggaggtg
     7261 tgccccttga ggccagcccc tgagcttcac cactctgccc ctgttcctgc caccagtgca
     7321 gttcctcggc tgcctcagct gcctcggcag aagtgcctgg tagctgcaaa cgcctgggga
     7381 acaggggagt cctgccgaca gggagcctgg actctagtct ccaacccctt ctgcccagag
     7441 cctagtcccc atcaagatcc atttctccct ggtgcctcag ccccccgggt ctgggctcag
     7501 ggccgcctcc aggggctggg atccattcat tcccgcacta acctaatgga ggctgagatc
     7561 ttggatgcag actcggactt cgccaccatg gactacaaag accatgacgg tgattataaa
     7621 gatcatgaca tcgattacaa ggatgacgat gacaaggcgg ccgcttagta agtaagatcc
     7681 gaactcgaga gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7741 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7801 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7861 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7921 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7981 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8041 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8101 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8161 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8221 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8281 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatcacctgc
     8401 aggacaggcg cgccctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8461 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8521 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8581 gaccctttta gtcagtgtgg aaaatctcta gcacccgggc gattaaggaa agggctagat
     8641 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8701 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8821 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8881 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8941 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9001 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9061 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     9121 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9181 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9241 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9301 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9361 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9421 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9481 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9541 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9601 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9661 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9721 caagtttact catatatact ttagattgat ttaaaacttc attttt
//
