LOCUS Exported 11696 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R. ACCESSION . VERSION . KEYWORDS pDestTol2-actinb-kcnj13-T131A-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11696) AUTHORS Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G TITLE Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish. JOURNAL Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. PUBMED 32546498 REFERENCE 2 (bases 1 to 11696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11696 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 5279..5303 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6429..6453) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 6452..6461 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6458..7174 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6458..7174 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6503..6524) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6764..6783) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7111..7132 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(7227..7246) /label=T7 /note="T7 promoter, forward primer" promoter complement(7228..7246) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 7251..7385 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(7448..7468) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(7476..7492) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal 7508..7642 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8314..8330) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8314..8330) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(8367..8387) /label=T3 /note="T3 promoter, forward primer" promoter complement(8367..8385) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8406..8422) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8419..8441) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8430..8446 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8454..8484) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8499..8520 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8637..8654) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8808..9396) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8888..8907) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9567..10427) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10190..10209 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10428..10532) /gene="bla" /label=AmpR promoter primer_bind complement(10695..10716) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 11612..11628 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 11648..11668 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" ORIGIN 1 gactcacctt ttcactgttg gtcttaattt aggttgatta tttttgtcaa tttaataatt 61 acattgtatt gttcatattt tattgtctta ttgtggtgtt tttacagctt ttttattgtt 121 ctgctttgtt actgtttgcc ttgtatgcat tgtagcgctt tgggtctgag aaaagcgcac 181 tataaataaa ctgtattatt attattaggg atggacatca cggtggcgcg atgggtggca 241 caattacctc acagcaagta ggttgctgtt tagagccccg gctggatcag ttggcagttc 301 tgtgtggagt ttgcatgttc tccctgttca agtaggtttc ctcccggtgc tctggtttcc 361 cctcacaagt ccaaagacat gatgtatagg tgaatggggt aagctaaatt gaccatagtg 421 agtgtgtgtg tgtgtgtgaa tgagagtgta taggtgtttc ccagtgttag gttgcaactg 481 gaaggacatc tgctgtgtaa aacatatgca ggataagttg gcggttcatt cccctatggt 541 gacccctggt taattaaagg actaagttga aaatgaatga atgaatgaat gattagagat 601 aaaccgatac cactttttta caaaccgata tgagtacaag tataatagtt tctgttactc 661 gacaatactg agtaccgatt ctgatacttc ttggatttgg tttcaatgta attaatttga 721 aagaaggttt tatttcttgc ctgtcgcaag gatgaacaca aaaatcttta ccagaaggat 781 attttaagct aagaatatac gcacgttgtt gacaaccctg tatcgattac cgattaaacg 841 tggacattta aaggataagt tatgcttgcg ccatcacaac tttacacatt acatgatcaa 901 agatcatatt gaatattcgg tttattcgtc agaagcctat agatttgtta aaagtgtatt 961 ttataaacgg atggtttata aagactaagc tattaatata tcagacattc attaatacat 1021 caagacatcg atgtcaggta ggacaacaaa tcacatccat gtaatgaaat cttgttgagt 1081 ggtattggtg cgtggtttca gcatttcatg tgcaatacga gtaagaatat tttaaccaag 1141 tatcgaccca aaacctgata ctggtatcgg tgcatccctt attattatta ttatcaaaaa 1201 cacacttgtt ttatttatgt gggtgccaaa caacaaacta tgaaatgcag catgacacgc 1261 ccaaaaagtg ttcctttgag aacaacttca taaatatgtt tcttatcatt atgcatgaat 1321 ggtgtactgg gagagctttg gtatcagaat tgagataaga gactttggca ttctaaagga 1381 caatgacatt atcacactga cagtcaacaa cactttattg ctctggaaat cttatgtcat 1441 ggggcaaagc tgaggttcat taggacagta ccttatttag agatgaatgg gaaggctgta 1501 gtgggctgct atcagggaag ggtggtggtc ctcgggggaa ctatggactc acaaaagcac 1561 atctgctgta acaagatccc ctgtgcaatc tacaaaagtt acaaagagaa gcatggttgg 1621 ttaaaaaata gaattgactg gatatgctga atgcattgtt tgttgtgtgt tacatcacat 1681 taaccacaga ttggtgctaa atatttgctt atcttataaa aacctgtgtg tttataaagg 1741 ggggtgtgga tggatgcttg gcattctttg aaaagatgtt gggggatgga aggaggagac 1801 cttattgatg agtatccagt ttgcagataa caagttttta gacattcata ctatgatgct 1861 gtttatgtct gttttttctc attttttcta tacacaaaca gctataaaac attcactttt 1921 atatctgatg ctgtatatta aatttcgttg tatttattct gtttgctctg ttgggtcata 1981 gctagggcca gacagaatct gctgacattt tttgcttttt ctgcagagaa ttttgtaaac 2041 aatctgtgat ttatgcgaga ttttttcggg agtatcgtaa ctaaaaactt actatatgaa 2101 ataaaaaaaa ataatacctt tttaactttt aataaatata tacaatgcaa gtccacctag 2161 atccaattat ttggtcgaca aagcaagtct ctcacataat atatcttcta aaagacagaa 2221 aacattactt tcagaccata ttgtaatcaa atgaacactt tcatattagt caataatatt 2281 acggaaatgt atttaaaaac tgaataaaca tatatttaca cctttgtctt tatttctaga 2341 gtgcttttac aatgtagatt gtgtcaaagc agcttaactt agaggattag tatagtaaat 2401 tgaaactgtg tcagtccatt ttttaagagt ttaagttcag ttcagtttag ttcagtgtga 2461 gttaattttt actgctgaga gtccaaacac taaagagcaa atccaaatac acaagtcaat 2521 aaataaactc aatgatgggc taaagatctg gggaaatctg cagatttctg tgcgcacaga 2581 ttctgtgtgg gcctagtcat tacctatact caacgtttac tctgagaaac ttccatattt 2641 tagaggtaaa atgctcagaa ttgtaaaaag tcttgtcaaa atacttggaa tttgccaaaa 2701 acaaaataaa agtcacatca cctcattgtc cttggtatca gcaagcactt ttaacacaat 2761 cagctgtttt tgtttataac tcccactgaa ggccatgtgc attactccca agttacacag 2821 taaaaagtca catgtagttt atcatgatca ctgctgctca agcacagctt tatctgttat 2881 tcagtgtggc aatatctgaa atgtgtgcaa aatattattt attgtagctg atcaccaaca 2941 ctttcagtgt tttttttttc tacttttaat gagaattatg ttcttaggat ttatccttat 3001 aaacctcttt aaaaagaaat gttatgtctg catagacacg acatgaaatt atgaacagaa 3061 aatttaacta tttaagatca agacttgaca tataacattt ctaatgaaca ttaaacctga 3121 ggtaggccca cttgcaaaaa tgtaactgct atcactcact ttatacatca ttgtgtaact 3181 tgtacacaca aaaatagtga agcaatgtgg catggaatgc aggtcccatt cattcaaagt 3241 tattaaagca ctttcttaaa gtgcattact actgagctaa gaccagctag ttgctcatac 3301 aacatactac aagtagtgtc atcctaattt ggataaaaaa aatgtccatt ttatacatgg 3361 aaaagtactc attagatccc ccattgtttg tattacggta tttcgtgaac acaagaggta 3421 aatgacctac agagctgctg ttgtgttaga ttgaaaacac aacacaggat catggaggca 3481 tccatcatta tgacgcattc tgccacttgt aactctgcac agactttttg aaaagtttaa 3541 ttgagtcttg gcgttgtcgc agaaaaatac gaaaaattct gtgttaaaat ttgcaaataa 3601 aaattccagt tttataggaa atcgccatgg ccttgttctt taaccagtta agcttcccct 3661 tctttcactc tcaagttgca agaagcaagt gtagcaatgt gcacgcgaca gccgggtgtg 3721 tgacgctgga ccaatcagag cacagagctc cgaaagttta ccttttatgg ctagagccgg 3781 gtatgtgccg tcatataaaa gagcgcgccc agcttttcag cctcactttg agctcctcca 3841 cacgcagcta gtgcggaata tcatctgctt gtaacccatt ctcttaagtc gacaaccccc 3901 caaacccaag gtgagttggt tttacagttt ttaagcgttt aattatagtt tctatctatt 3961 aatgttaagt aaaatgtgta aatggataaa tatgatttga tgaagtcttt agacagttca 4021 taacggtctc tttgtagtta tctactaggt ttgatttata agtggtggtt cttttgataa 4081 tctatgttac atttaataaa tattggctgt atcttaactt ttttgtagcg ttagtggatg 4141 tggcaggtga gaatgtcggt aatttaacgt gaccagtcgt aggcacgaca ttgaacattg 4201 aataggccgg tgtgaaatgg gtgttaagtc ttaatataaa gttgtgttct ggctaatctc 4261 tctttaacag ccataaatgt gatttttaac tacagttttg agggtgctgg tgagcatgtt 4321 gcacacttga tggatagccg gcatgggaag ttctttgtgc aggcagtgct gcagcagggt 4381 gtgacctact ttagctagcc ggctaaccag ctctcatctg atgtaacgta aaccccatca 4441 atctaacatc ggctcattcg tgctttataa gatcgaagat cttgttatta gtaacgggta 4501 ttggcattta gttttaactt ggttaatttt ctttttgtta tgtgttctat ttaaatgtag 4561 cctgcagccc cctctgtctt taatgaagct gaagattaac gttaacgtta gtgaagtgtt 4621 gtatccgcta tgaaagattt gaattttaga ggtgtggtcc actttaataa cggctgcgga 4681 gttctctggc tgcacttcag ccgctgtgac tgtcgaagca gagcccatca gtttttcagg 4741 atctcggtga tttttgaggc tagactatga actgaaccga ctgagagtga ccgtccagaa 4801 atgctttagt gtgaagtctc cgctcacgag gagtccgtgg actgactgca gatctgtgac 4861 gcagtaacac ccggcggcag acacccgttg aaatgcggtt gtgtaattga tcgcaggcga 4921 gggtggaaga ggatgtgaaa cttcatttgt gtaaaattta gggagtggtc ctggtgtgat 4981 gaatgtcgaa atctgttcct ttttactgag ccctacgact ctggctgagt gccacaccgc 5041 cggcagccgc aaagcggctc aaaccattgc cttttatggt aataatgaga gaatgcagag 5101 ggacttcctt tgtctggcat atctgaggcg cgcgctgtca ctagcgccca ccagcggtca 5161 gactgtagaa tgcagagcaa aacaggaagt tgactccaga tggtcacatg cttgctgaaa 5221 cgcctctccc tggcagcagt gcaattctaa cagtttccct ttcttttaca gttcagcctc 5281 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatgccta ccaccatgac 5341 aaacaccacc gctgaccaaa aggcctcctg ccctttgatg gtcaaacctc agcgtcgccg 5401 cctggtcagc aaagacggtc ggagccagac aagaggaaac acaagaggag gctccagaga 5461 gacgtgtttc agtgctctgc gggacttgtg gggcacatgg ctggcgctac ggtggcggtg 5521 ggtggttttg gcattttgtg gctcattcct gcttcactgg ctgctgtttg ctgtattatg 5581 gtacctgctg gctcgggtca atggagacct ggatgtcctg gatcatgatt ctcctcctcc 5641 cggtcatgtg ctgtgtgtta aacatgtgaa tgggttcaca gccgcttttt cattcgccct 5701 ggagacgcaa ctcgctattc ggtatggcac aatgtaccca aatgcagact gtcccactgc 5761 catcgcgctg cttgccctgc agatgctgct gggactcatg ctggaggcct ttatcactgg 5821 tgcatttgta gccaagttct ctcggcctca gaagcgatgt gacgggatcc tattcagccc 5881 tcaggctgtg gtgtgtgagc agaaaggcca gcgctgtcta atgtttcgag tgtgtaacct 5941 ccagccacag ccgctagtgg acgtatccgt gagtgctgtc ctctacgagg agcgagacga 6001 tcacgaactc caccaaactg ctctggagtt cagcatcgac aacctgggct ccagatcttg 6061 ccctcttttc ctctcccctc tgaccttctt tcacccttta aatccatcca ctcctttcat 6121 caacaaccca agcagccaaa cacactttga gcttgtagtg tttctcactg cgactcagga 6181 aagcacaggg tctggttacc acaagaggac gtcttacctg cctgacgaga tccaatatgg 6241 ctactgcttc tccaaggtga cgtctgtaca ccagaacaaa accccaaaca tgcgctattt 6301 tgacacggtg ccctgtcccc tcacactagc caacacacac accactgata caccggataa 6361 agagcatgtg gtggttcagc tgaatggaga aggcagcgac cgcgtggaga agaagggtgg 6421 gcgcgccgac ccagctttct tgtacaaagt ggccaccatg gtgagcaagg gcgaggagct 6481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 6541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 6601 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 6661 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 6721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 6781 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 6841 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 6901 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 6961 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 7021 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 7081 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 7141 cgggatcact ctcggcatgg acgagctgta caagggcggt ggaagatctg ggaattcaag 7201 gcctctcgag cctctagatt ctgcagccct atagtgagtc gtattacgta gatccagaca 7261 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 7321 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 7381 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 7441 ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat 7501 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat 7561 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 7621 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 7681 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga 7741 ttatgatcct ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc 7801 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc 7861 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa 7921 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata 7981 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg 8041 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa 8101 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa 8161 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc 8221 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg 8281 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca 8341 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa 8401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 8461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 8521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 8581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 8641 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 8701 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 8761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 8821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 8881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 8941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 9001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 9061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 9121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 9181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 9241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 9301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 9361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 9421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca 9481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 9541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 9601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 9661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 9721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 9781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 9841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 9901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 9961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 10021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 10081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 10141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 10201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 10261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 10321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 10381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 10441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 10501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa 10561 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 10621 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 10681 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 10741 tcaaagggcg aaaaaccgtc tatcagggcg atggcccacg ctggctaaga actcatcagc 10801 ctccccggtc catctaccca cgtaccaatg caccaattgg ccacaatgac ggctactaca 10861 tggtgccatt ccttcctctt tataggaatg gagactacct cctgtccaac aatgctcttg 10921 gatacgagta cgcctacctg ttggacccag gtcattgcac aacaccagaa atgccctctg 10981 atctgcaaaa gacgtgaata tctgttcaga cacccatatc cactctgttc cacacaggtc 11041 agaggtttgt ccaggagttc ttgacagagg tgtaaaaagt actcaaaaat tttactcaag 11101 tgaaagtaca agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga 11161 atcttacttg agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa 11221 gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt taagctttta atctcaaaaa 11281 acattaaatg aaatgcatac aaggttttat cctgctttag aactgtttgt atttaattat 11341 caaactataa gacagacaat ctaatgccag tacacgctac tcaaagttgt aaaacctcag 11401 atttaacttc agtagaagct gattctcaaa attgttagtg tcaagcctag ctcttttggg 11461 gctgaaaagc aatcctgcag tgctgaaaag cctctcacag gcagccgatg cgggaagagg 11521 tgtattagtc ttgatagaga ggctgcaaat agcaggaaac gtgagcagag actccctggt 11581 gtctgaaaca caggccagat gggccctcga gcaggaaaca gctatgacca tgattacgcc 11641 aagctatcaa ctttgtatag aaaagttgaa aattccagtt tgaagaaact tttcaa //