LOCUS Exported 2291 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0398 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2291) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2291) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2291 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 184..203 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 431..449 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 453..496 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 763..820 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(844..861) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1015..1603) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1095..1114) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1786..1880 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1901..269) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga 61 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt 121 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat 181 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg 241 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat 301 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt 361 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt 421 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt 481 cgctaaggat gatttctgga attcggtctc aggagggagg cgtgtcgttt tagtgaggta 541 tgcccaattg aagataagat aataatgttg aagataagag tgggagccac catgcccaat 601 taaagacctc tagctttgtc aaaagctaaa aaagatgatg ctcacgacca caattaggtt 661 gatgacgtaa gccatgacgt ctacatgtag gctctatata aggttttgct attcattgaa 721 agcagtagtg actgatttgt atatatactc gagaccctgc agtccggcaa aaaagggcaa 781 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc 841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1021 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 1081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 1141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 1201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 1261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 1321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 1381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1441 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1741 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa 1801 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac 1861 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact 1921 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 1981 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 2041 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 2101 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 2161 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 2221 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 2281 cggtgtaaca a //