LOCUS       Exported               10872 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Fluorescent reporter for F-actin labeling in living cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-Lifeact-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2576..3787
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3624..3643
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3877..3927
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      3934..3943
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3940..5370
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5518..6106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5571..5591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5989..6000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6109..6125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6110..6126)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6631..6811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6837..6854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6843..7067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7113..7541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7200..7219)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7410..7431
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7550..7570)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7555..7884
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7735..7870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7797..7816
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7932..7979
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7998..8372
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8502..8623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8539..8558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8593..8612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8672..8688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8672..8688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8685..8707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8696..8712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8720..8750)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8765..8786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8903..8920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9074..9662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9154..9173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9833..10693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10456..10475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10694..10798)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct
     2581 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     2641 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     2701 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     2761 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     2821 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     2881 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     2941 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     3001 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     3061 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     3121 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     3181 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     3241 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     3301 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     3361 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     3421 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     3481 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     3541 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     3601 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     3661 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     3721 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     3781 gttagacgaa gcttgggctg caggtcgact ctagaggatc gaattcttcg aagctagcgc
     3841 taccggactc agatctcgag ctcaagcttc gaattcatgg gcgtggccga cctgatcaag
     3901 aagttcgaga gcatcagcaa ggaggagccg gtcgccacca tggtgagcaa gggcgaggag
     3961 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     4021 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4081 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     4141 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     4201 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     4261 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     4321 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4381 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     4441 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     4501 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     4561 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
     4621 catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
     4681 atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     4741 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     4801 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     4861 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     4921 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     4981 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     5041 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5101 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     5161 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     5221 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     5281 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     5341 ctgtacggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
     5401 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5461 gaaacataaa atgaatgcaa ttgttgttgt taacaattcg atatcaagct tatcgataat
     5521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6121 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6181 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6241 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6301 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6361 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6421 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6481 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6541 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6601 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6661 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6721 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6781 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     6841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7021 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7081 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7561 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7621 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7681 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7801 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7861 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7921 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     7981 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     8041 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     8101 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     8161 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     8221 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     8281 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     8341 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     8401 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8461 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8581 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8641 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8701 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8761 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8821 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8881 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8941 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9001 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9061 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9121 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9181 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9241 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9301 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9361 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9421 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9481 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9541 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9601 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9661 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9721 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9781 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9841 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9901 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9961 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10021 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10081 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10141 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10201 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10261 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10321 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10381 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10441 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10501 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10561 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10621 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10681 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10741 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10801 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10861 gtgcactctc ag
//
