LOCUS Exported 10633 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.. ACCESSION . VERSION . KEYWORDS Tet-pLKO-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10633) AUTHORS Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD TITLE Single-vector inducible lentiviral RNAi system for oncology target validation. JOURNAL Cell Cycle. 2009 Feb 1. 8(3):498-504. PUBMED 19177017 REFERENCE 2 (bases 1 to 10633) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10633 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 42..59 /label=L4440 /note="L4440 vector, forward primer" protein_bind 176..197 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 212..242 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 250..266 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 255..277 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 274..290 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 274..290 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 309..329 /label=T3 /note="T3 promoter, forward primer" promoter 311..329 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 357..583 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 584..764 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 811..936 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1429..1662 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1847..1891 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2180..2274 /label=H1-2O2 promoter /note="doxycycline-inducible variant of the human H1 RNA promoter (Henriksen et al., 2007)" primer_bind 2191..2210 /label=H1 /note="Human H1 promoter, forward primer" protein_bind 2228..2246 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2256..2274 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 4192..4309 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 4358..4868 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 4787..4805 /label=hPGK-F /note="Human PGK promoter, forward primer" intron 4877..5449 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 5457..5476 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" promoter 5504..5522 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 5533..6150 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG" primer_bind complement(5606..5625) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" misc_feature 6188..6761 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6355..6372) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6582..6601 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6781..7380 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6781..6800) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7277..7297 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7508..7741 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7813..7934 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7850..7869) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7904..7923 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7974..8109 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8036..8055 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter complement(8130..8148) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8158..8175) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8158..8174) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8167..8189) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8316..8771 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8403..8422) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8613..8634 /label=F1ori-F /note="F1 origin, forward primer" promoter 8797..8901 /gene="bla" /label=AmpR promoter CDS 8902..9762 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9120..9139) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9933..10521 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10422..10441 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 61 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 301 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta 361 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt 421 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc 481 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca 541 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga 601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg 781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa 841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 1261 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg 1321 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac 1381 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt 1441 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt 1501 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat 1561 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag 1621 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc 1681 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct 1741 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 1801 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 1861 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 1921 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 1981 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 2041 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 2101 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga 2161 ctagcctcga gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa 2221 cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg 2281 tgtgttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta tgacctttat 2341 aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa tttttatgtt 2401 cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc 2461 gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa gcggtcagct 2521 cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc tgctttaact 2581 aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt tgcacctgat 2641 tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc tatttcacaa 2701 gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt aacaacatgt 2761 gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata atttttgtct 2821 tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt aattgcttca 2881 gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc tacaacattt 2941 gttattccta catttgtgcc ttttaataat tctttacaat agcttgttca atatgaatta 3001 acacaaactg ttgcaaaatc aaattcaatt gcttcatcac ataattgttt aatttcagct 3061 ttcgtagcat cttgttttaa taatgtgtga tctatatatt tgtttagttt cattttttct 3121 cctatatatt catttttaat tttaattctt taataatttc gtctacttta actttagcgt 3181 tttgaacaga ttcaccaaca cctataaaat aaatttttag tttaggttca gttccacttg 3241 ggcgaacagc aaatcatgac ttatcttcta aataaaattt tagtaagtct tgtcctggca 3301 tattatacat tccatcgatg tagtcttcaa cattaacaac tttaagtcca gcaatttgag 3361 ttaagggtgt tgctctcaat gatttcatta atggttcaat ttttaatttc ttttcttctg 3421 gtttaaaatt caagtttaaa gtgaaagtgt aatatgcacc catttcttta aataaatctt 3481 ctaaatagtc tactaatgtt ttattttgtt ttttataaaa tcaagcagcc tctgctatta 3541 atatagaagc ttgtattcca tctttatctc tagctgagtc atcaattaca tatccataac 3601 tttcttcata agcaaaaaca aaatttaatc cgttatcttc ttctttagca atttctctac 3661 ccattcattt aaatccagtt aaagttttta caatattaac tccatatttt tcatgagcga 3721 ttctatcacc caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc 3781 tatttaagcg ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc 3841 catctaatct tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg 3901 ggtcattcat aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc 3961 atgcaggatc aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg 4021 catgctcttc aacctcaata acgttatatc ctgattcacg taatattttt ggggtaaatt 4081 tagttcctgt tccattaact gcgctaaaaa taatttttaa atctttttta gcttcttgct 4141 cttttttgta gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag 4201 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4261 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta 4321 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc 4381 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca 4441 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct 4501 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag 4561 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta 4621 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg 4681 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg 4741 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct 4801 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct 4861 ctccccaggg ggatctgtga gtttggggac ccttgattgt tctttctttt tcgctattgt 4921 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg 4981 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg 5041 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag 5101 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt 5161 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt 5221 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg 5281 ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt 5341 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa 5401 accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 5461 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg actcactata 5521 gggcgaattg atatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg 5581 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 5641 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc 5701 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 5761 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 5821 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 5881 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct 5941 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 6001 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 6061 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 6121 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gcggctcccg ggagttctag 6181 ggatctgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 6241 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 6301 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 6361 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 6421 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 6481 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 6541 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 6601 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 6661 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 6721 accacgggga cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc 6781 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6841 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6901 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6961 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 7021 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 7081 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 7141 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 7201 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 7261 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 7321 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 7381 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggta 7441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 7501 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 7561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 7621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 7681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 7741 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 7801 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 7861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 7921 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc 7981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 8041 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 8101 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 8161 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 8221 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 8281 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag 8341 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 8401 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 8461 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 8521 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 8581 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 8641 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 8701 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 8761 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 8821 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 8881 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 8941 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 9001 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 9061 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 9121 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 9181 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 9241 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 9301 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 9361 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 9421 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 9481 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 9541 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 9601 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 9661 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 9721 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 9781 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 9841 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 9901 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 9961 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 10021 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 10081 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 10141 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 10201 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 10261 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 10321 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 10381 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 10441 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 10501 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 10561 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 10621 attaccgcct ttg //