LOCUS       Exported                9143 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Doxycycline-inducible all-in-one lentivirus vector to express 
            H2BeGFP fusion protein. System for labelling slow-cycling cells in 
            vivo..
ACCESSION   .
VERSION     .
KEYWORDS    pSIN-TRE-H2BeGFP-rtTA2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Puig I, Tenbaum SP, Chicote I, Arques O, Martinez-Quintanilla J, 
            Cuesta-Borras E, Ramirez L, Gonzalo P, Soto A, Aguilar S, Eguizabal 
            C, Caratu G, Prat A, Argiles G, Landolfi S, Casanovas O, Serra V, 
            Villanueva A, Arroyo AG, Terracciano L, Nuciforo P, Seoane J, Recio 
            JA, Vivancos A, Dienstmann R, Tabernero J, Palmer HG
  TITLE     TET2 controls chemoresistant slow-cycling cancer cell survival and 
            tumor recurrence.
  JOURNAL   J Clin Invest. 2018 Aug 31;128(9):3887-3905. doi: 10.1172/JCI96393. 
            Epub 2018 Aug 6.
  PUBMED    29944140
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(54..74)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(472..483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             676..909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    981..1102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1018..1037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1072..1091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1142..1277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1204..1223
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(1297..1316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1298..1316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1326..1343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1326..1342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1335..1357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1484..1939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1571..1590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1781..1802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1965..2069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2070..2930
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2288..2307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3101..3689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3590..3609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3843..3860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3977..3998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4013..4043
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4051..4067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4056..4078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4075..4091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4075..4091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4110..4130
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4112..4130
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4158..4384
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4385..4565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4612..4737
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5230..5463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5648..5692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5959..6076
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6109..6379
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        6412..6450
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6447..6471
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6595..6972
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6985..6994
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6991..7710
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6991..7707
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7036..7057)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7297..7316)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7644..7665
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        7766..8276
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8195..8213
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8314..9060
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
       61 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
      241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
      301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
      361 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
      421 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
      481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
      541 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
      601 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
      661 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
      721 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      781 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      841 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      901 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
      961 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     1021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1081 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     1141 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1201 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     1261 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     1321 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1381 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     1501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1681 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1801 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1921 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2281 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3241 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3481 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3781 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3841 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3901 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3961 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4021 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4081 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     4141 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     4201 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     4261 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     4321 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     4381 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4441 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4501 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4561 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     4621 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     4681 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     4741 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     4801 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     4861 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     4921 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     4981 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     5041 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     5101 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     5161 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     5221 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     5281 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     5341 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     5401 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     5461 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     5521 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     5581 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     5641 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     5701 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     5761 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     5821 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     5881 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     5941 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     6001 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     6061 aaaaattcaa aattttatcg atcacgagac tagcctcgag tttaccactc cctatcagtg
     6121 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     6181 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     6241 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     6301 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     6361 tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt
     6421 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     6481 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccgcggc
     6541 cccgaattcg agctcggtac ccggggatcc tctagtcagc tgacgcgtgc tagaatgccc
     6601 gagccagcga agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg
     6661 cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtg
     6721 tacaaggttc tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc
     6781 atgaattcgt ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg
     6841 cattacaaca agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg
     6901 ctgcctgggg agttggccaa gcacgccgtg tccgagggta ctaaggccat caccaagtac
     6961 accagcgcta aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     7021 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7081 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7141 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7201 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7261 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7321 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7381 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7441 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7501 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7561 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7621 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7681 actctcggca tggacgagct gtacaagtaa gcgatcgcgc tagcgcggcc gcatcgataa
     7741 gcttgtcgac gatatcgaat tccacggggt tggggttgcg ccttttccaa ggcagccctg
     7801 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     7861 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     7921 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     7981 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     8041 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     8101 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     8161 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     8221 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     8281 atccgtttat ctgcagaatt cgcccttgac gtcatgtcta gactggacaa gagcaaagtc
     8341 ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     8401 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     8461 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     8521 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     8581 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     8641 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     8701 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     8761 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     8821 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     8881 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     8941 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     9001 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     9061 cggaccggac gtcaagggcg aattccagca cactggcggc cgttactagc ttctgcagca
     9121 cgtgtctaga aacgctggtc gac
//
