LOCUS       Exported               13347 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-ATR WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Jiang G, Sancar A
  TITLE     Recruitment of DNA damage checkpoint proteins to damage in 
            transcribed and nontranscribed sequences.
  JOURNAL   Mol Cell Biol. 2006 Jan . 26(1):39-49.
  PUBMED    16354678
REFERENCE   2  (bases 1 to 13347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             941..964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8938..8955)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8944..9168
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9214..9642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9301..9320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9511..9532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9651..9671)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9656..9985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9836..9971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9898..9917
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10052..10846
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10106..10125)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10716..10735
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11020..11141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11057..11076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11111..11130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11190..11206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11190..11206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11203..11225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11214..11230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11238..11268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11283..11304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11421..11438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11592..12180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11672..11691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12351..13211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12974..12993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13212..13316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccaggatg gactacaagg acgacgatga
      961 caagggggaa catggcctgg agctggcttc catgatcccc gccctgcggg agctgggcag
     1021 tgccacacca gaggaatata atacagttgt acagaagcca agacaaattc tgtgtcaatt
     1081 cattgaccgg atacttacag atgtaaatgt tgttgctgta gaacttgtaa agaaaactga
     1141 ctctcagcca acctccgtga tgttgcttga tttcatccag catatcatga aatcctcccc
     1201 acttatgttt gtaaatgtga gtggaagcca tgaggccaaa ggcagttgta ttgaattcag
     1261 taattggatc ataacgagac ttctgcggat tgcagcaact ccctcctgtc atttgttaca
     1321 caagaaaatc tgtgaagtca tctgttcatt attatttctt tttaaaagca agagtcctgc
     1381 tatttttggg gtcctcacaa aagaattatt acaacttttt gaagacttgg tttacctcca
     1441 tagaagaaat gtgatgggtc atgctgtgga atggccagtg gtcatgagcc gatttttaag
     1501 tcaattagat gaacacatgg gatatttaca atcagctcct ttgcagttga tgagtatgca
     1561 aaatttagaa tttattgaag tcactttatt aatggttctt actcgtatta ttgcaattgt
     1621 gttttttaga aggcaagaac tcttactttg gcagataggt tgtgttctgc tagagtatgg
     1681 tagtccaaaa attaaatccc tagcaattag ctttttaaca gaactttttc agcttggagg
     1741 actaccagca caaccagcta gcactttttt cagctcattt ttggaattat taaaacacct
     1801 tgtagaaatg gatactgacc aattgaaact ctatgaagag ccattatcaa agctgataaa
     1861 gacactattt ccctttgaag cagaagctta tagaaatatt gaacctgtct atttaaatat
     1921 gctgctggaa aaactctgtg tcatgtttga agacggtgtg ctcatgcggc ttaagtctga
     1981 tttgctaaaa gcagctttgt gccatttact gcagtatttc cttaaatttg tgccagctgg
     2041 gtatgaatct gctttacaag tcaggaaggt ctatgtgaga aatatttgta aagctctttt
     2101 ggatgtgctt ggaattgagg tagatgcaga gtacttgttg ggcccacttt atgcagcttt
     2161 gaaaatggaa agtatggaaa tcattgagga gattcaatgc caaactcaac aggaaaacct
     2221 cagcagtaat agtgatggaa tatcacccaa aaggcgtcgt ctcagctcgt ctctaaaccc
     2281 ttctaaaaga gcaccaaaac agactgagga aattaaacat gtggacatga accaaaagag
     2341 catattatgg agtgcactga aacagaaagc tgaatccctt cagatttccc ttgaatacag
     2401 tggcctaaag aatcctgtta ttgagatgtt agaaggaatt gctgttgtct tacaactgac
     2461 tgctctgtgt actgttcatt gttctcatca aaacatgaac tgccgtactt tcaaggactg
     2521 tcaacataaa tccaagaaga aaccttctgt agtgataact tggatgtcat tggattttta
     2581 cacaaaagtg cttaagagct gtagaagttt gttagaatct gttcagaaac tggacctgga
     2641 ggcaaccatt gataaggtgg tgaaaattta tgatgctttg atttatatgc aagtaaacag
     2701 ttcatttgaa gatcatatcc tggaagattt atgtggtatg ctctcacttc catggattta
     2761 ttcccattct gatgatggct gtttaaagtt gaccacattt gccgctaatc ttctaacatt
     2821 aagctgtagg atttcagata gctattcacc acaggcacaa tcacgatgtg tgtttcttct
     2881 gactctgttt ccaagaagaa tattccttga gtggagaaca gcagtttaca actgggccct
     2941 gcagagctcc catgaagtaa tccgggctag ttgtgttagt ggatttttta tcttattgca
     3001 gcagcagaat tcttgtaaca gagttcccaa gattcttata gataaagtca aagatgattc
     3061 tgacattgtc aagaaagaat ttgcttctat acttggtcaa cttgtctgta ctcttcacgg
     3121 catgttttat ctgacaagtt ctttaacaga acctttctct gaacacggac atgtggacct
     3181 cttctgtagg aacttgaaag ccacttctca acatgaatgt tcatcttctc aactaaaagc
     3241 ttctgtctgc aagccattcc ttttcctact gaaaaaaaaa atacctagtc cagtaaaact
     3301 tgctttcata gataatctac atcatctttg taagcatctt gattttagag aagatgaaac
     3361 agatgtaaaa gcagttcttg gaactttatt aaatttaatg gaagatccag acaaagatgt
     3421 tagagtggct tttagtggaa atatcaagca catattggaa tccttggact ctgaagatgg
     3481 atttataaag gagctttttg tcttaagaat gaaggaagca tatacacatg cccaaatatc
     3541 aagaaataat gagctgaagg ataccttgat tcttacaaca ggggatattg gaagggccgc
     3601 aaaaggagat ttggtaccat ttgcactctt acacttattg cattgtttgt tatccaagtc
     3661 agcatctgtc tctggagcag catacacaga aattagagct ctggttgcag ctaaaagtgt
     3721 taaactgcaa agttttttca gccagtataa gaaacccatc tgtcagtttt tggtagaatc
     3781 ccttcactct agtcagatga cagcacttcc gaatactcca tgccagaatg ctgacgtgcg
     3841 aaaacaagat gtggctcacc agagagaaat ggctttaaat acgttgtctg aaattgccaa
     3901 cgttttcgac tttcctgatc ttaatcgttt tcttactagg acattacaag ttctactacc
     3961 tgatcttgct gccaaagcaa gccctgcagc ttctgctctc attcgaactt taggaaaaca
     4021 attaaatgtc aatcgtagag agattttaat aaacaacttc aaatatattt tttctcattt
     4081 ggtctgttct tgttccaaag atgaattaga acgtgccctt cattatctga agaatgaaac
     4141 agaaattgaa ctggggagcc tgttgagaca agatttccaa ggattgcata atgaattatt
     4201 gctgcgtatt ggagaacact atcaacaggt ttttaatggt ttgtcaatac ttgcctcatt
     4261 tgcatccagt gatgatccat atcagggccc gagagatatc atatcacctg aactgatggc
     4321 tgattattta caacccaaat tgttgggcat tttggctttt tttaacatgc agttactgag
     4381 ctctagtgtt ggcattgaag ataagaaaat ggccttgaac agtttgatgt ctttgatgaa
     4441 gttaatggga cccaaacatg tcagttctgt gagggtgaag atgatgacca cactgagaac
     4501 tggccttcga ttcaaggatg attttcctga attgtgttgc agagcttggg actgctttgt
     4561 tcgctgcctg gatcatgctt gtctgggctc ccttctcagt catgtaatag tagctttgtt
     4621 acctcttata cacatccagc ctaaagaaac tgcagctatc ttccactacc tcataattga
     4681 aaacagggat gctgtgcaag attttcttca tgaaatatat tttttacctg atcatccaga
     4741 attaaaaaag ataaaagccg ttctccagga atacagaaag gagacctctg agagcactga
     4801 tcttcagaca actcttcagc tctctatgaa ggccattcaa catgaaaatg tcgatgttcg
     4861 tattcatgct cttacaagct tgaaggaaac cttgtataaa aatcaggaaa aactgataaa
     4921 gtatgcaaca gacagtgaaa cagtagaacc tattatctca cagttggtga cagtgctttt
     4981 gaaaggttgc caagatgcaa actctcaagc tcggttgctc tgtggggaat gtttagggga
     5041 attgggggcg atagatccag gtcgattaga tttctcaaca actgaaactc aaggaaaaga
     5101 ttttacattt gtgactggag tagaagattc aagctttgcc tatggattat tgatggagct
     5161 aacaagagct taccttgcgt atgctgataa tagccgagct caagattcag ctgcctatgc
     5221 cattcaggag ttgctttcta tttatgactg tagagagatg gagaccaacg gcccaggtca
     5281 ccaattgtgg aggagatttc ctgagcatgt tcgggaaata ctagaacctc atctaaatac
     5341 cagatacaag agttctcaga agtcaaccga ttggtctgga gtaaagaagc caatttactt
     5401 aagtaaattg ggtagtaact ttgcagaatg gtcagcatct tgggcaggtt atcttattac
     5461 aaaggttcga catgatcttg ccagtaaaat tttcacctgc tgtagcatta tgatgaagca
     5521 tgatttcaaa gtgaccatct atcttcttcc acatattctg gtgtatgtct tactgggttg
     5581 taatcaagaa gatcagcagg aggtttatgc agaaattatg gcagttctaa agcatgacga
     5641 tcagcatacc ataaataccc aagacattgc atctgatctg tgtcaactca gtacacagac
     5701 tgtgttctcc atgcttgacc atctcacaca gtgggcaagg cacaaatttc aggcactgaa
     5761 agctgagaaa tgtccacaca gcaaatcaaa cagaaataag gtagactcaa tggtatctac
     5821 tgtggattat gaagactatc agagtgtaac ccattttcta gacctcatac cccaggatac
     5881 tctggcagta gcttcctttc gctccaaagc atacacacga gctgtaatgc actttgaatc
     5941 atttattaca gaaaagaagc aaaatattca ggaacatctt ggatttttac agaaattgta
     6001 tgctgctatg catgaacctg atggagtggc cggagtcagt gcaattagaa aggcagaacc
     6061 atctctaaaa gaacagatcc ttgaacatga aagccttggc ttgctgaggg atgccactgc
     6121 ttgttatgac agggctattc agctagaacc agaccagatc attcattatc atggtgtagt
     6181 aaagtccatg ttaggtcttg gtcagctgtc tactgttatc actcaggtga atggagtgca
     6241 tgctaacagg tccgagtgga cagatgaatt aaacacgtac agagtggaag cagcttggaa
     6301 attgtcacag tgggatttgg tggaaaacta tttggcagca gatggaaaat ctacaacatg
     6361 gagtgtcaga ctgggacagc tattattatc agccaaaaaa agagatatca cagcttttta
     6421 tgactcactg aaactagtga gagcagaaca aattgtacct ctttcagctg caagctttga
     6481 aagaggctcc taccaacgag gatatgaata tattgtgaga ttgcacatgt tatgtgagtt
     6541 ggagcatagc atcaaaccac ttttccagca ttctccaggt gacagttctc aagaagattc
     6601 tctaaactgg gtagctcgac tagaaatgac ccagaattcc tacagagcca aggagcctat
     6661 cctggctctc cggagggctt tactaagcct caacaaaaga ccagattaca atgaaatggt
     6721 tggagaatgc tggctgcaga gtgccagggt agctagaaag gctggtcacc accagacagc
     6781 ctacaatgct ctccttaatg caggggaatc acgactcgct gaactgtacg tggaaagggc
     6841 aaagtggctc tggtccaagg gtgatgttca ccaggcacta attgttcttc aaaaaggtgt
     6901 tgaattatgt tttcctgaaa atgaaacccc acctgagggt aagaacatgt taatccatgg
     6961 tcgagctatg ctactagtgg gccgatttat ggaagaaaca gctaactttg aaagcaatgc
     7021 aattatgaaa aaatataagg atgtgaccgc gtgcctgcca gaatgggagg atgggcattt
     7081 ttaccttgcc aagtactatg acaaattgat gcccatggtc acagacaaca aaatggaaaa
     7141 gcaaggtgat ctcatccggt atatagttct tcattttggc agatctctac aatatggaaa
     7201 tcagttcata tatcagtcaa tgccacgaat gttaactcta tggcttgatt atggtacaaa
     7261 ggcatatgaa tgggaaaaag ctggccgctc cgatcgtgta caaatgagga atgatttggg
     7321 taaaataaac aaggttatca cagagcatac aaactattta gctccatatc aatttttgac
     7381 tgctttttca caattgatct ctcgaatttg tcattctcac gatgaagttt ttgttgtctt
     7441 gatggaaata atagccaaag tatttctagc ctatcctcaa caagcaatgt ggatgatgac
     7501 agctgtgtca aagtcatctt atcccatgcg tgtgaacaga tgcaaggaaa tcctcaataa
     7561 agctattcat atgaaaaaat ccttagagaa gtttgttgga gatgcaactc gcctaacaga
     7621 taagcttcta gaattgtgca ataaaccggt tgatggaagt agttccacat taagcatgag
     7681 cactcatttt aaaatgctta aaaagctggt agaagaagca acatttagtg aaatcctcat
     7741 tcctctacaa tcagtcatga tacctacact tccatcaatt ctgggtaccc atgctaacca
     7801 tgctagccat gaaccatttc ctggacattg ggcctatatt gcagggtttg atgatatggt
     7861 ggaaattctt gcttctcttc agaaaccaaa gaagatttct ttaaaaggct cagatggaaa
     7921 gttctacatc atgatgtgta agccaaaaga tgacctgaga aaggattgta gactaatgga
     7981 attcaattcc ttgattaata agtgcttaag aaaagatgca gagtctcgta gaagagaact
     8041 tcatattcga acatatgcag ttattccact aaatgatgaa tgtgggatta ttgaatgggt
     8101 gaacaacact gctggtttga gacctattct gaccaaacta tataaagaaa agggagtgta
     8161 tatgacagga aaagaacttc gccagtgtat gctaccaaag tcagcagctt tatctgaaaa
     8221 actcaaagta ttccgagaat ttctcctgcc caggcatcct cctatttttc atgagtggtt
     8281 tctgagaaca ttccctgatc ctacatcatg gtacagtagt agatcagctt actgccgttc
     8341 cactgcagta atgtcaatgg ttggttatat tctggggctt ggagaccgtc atggtgaaaa
     8401 tattctcttt gattctttga ctggtgaatg cgtacatgta gatttcaatt gtcttttcaa
     8461 taagggagaa acctttgaag ttccagaaat tgtgccattt cgcctgactc ataatatggt
     8521 taatggaatg ggtcctatgg gaacagaggg tctttttcga agagcatgtg aagttacaat
     8581 gaggctgatg cgtgatcagc gagagccttt aatgagtgtc ttaaagactt ttctacatga
     8641 tcctcttgtg gaatggagta aaccagtgaa agggcattcc aaagcgccac tgaatgaaac
     8701 tggagaagtt gtcaatgaaa aggccaagac ccatgttctt gacattgagc agcgactaca
     8761 aggtgtaatc aagactcgaa atagagtgac aggactgccg ttatctattg aaggacatgt
     8821 gcattacctt atacaggaag ctactgatga aaacttacta tgccagatgt atcttggttg
     8881 gactccatat atgtgactcg agtctagcta gagggcccgt ttaaacccgc tgatcagcct
     8941 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9001 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9061 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9121 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9181 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9241 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9301 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9361 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9421 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9481 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9541 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9601 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9901 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9961 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
    10021 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
    10081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    10141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    10201 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    10261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10561 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10861 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    10921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10981 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    11041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11101 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    11161 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    12061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13321 tttccccgaa aagtgccacc tgacgtc
//
