LOCUS       Exported                9151 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Perturb-seq vector backbone.
ACCESSION   .
VERSION     .
KEYWORDS    pBA439
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE,
            Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit 
            A, Parnas O, Regev A, Weissman JS
  TITLE     A Multiplexed Single-Cell CRISPR Screening Platform Enables 
            Systematic Dissection of the Unfolded Protein Response.
  JOURNAL   Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 
            10.1016/j.cell.2016.11.048.
  PUBMED    27984733
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(26..622)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(106..126)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     603..622
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          643..1581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(666..686)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(1582..1793)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(2038..2058)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2062..2095)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    2309..2342
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2398..2986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2451..2471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2869..2880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2989..3005)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2990..3006)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3515..3695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3837..3856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4516..5376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5139..5158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5377..5481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5556..5575)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5747..6126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6128..6326
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6281..6301
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6344..6524
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6571..6696
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7193..7426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7611..7655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7953..8070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    8129..8353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8342..8359
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8403..9101)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(9114..16)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gacttcctct gccctctccc gatccggcac cgggcttgcg ggtcatgcac caggtgcgcg
       61 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
      121 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
      181 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
      241 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
      301 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
      361 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
      421 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
      481 cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg
      541 cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca
      601 ccgtgggctt gtactcggtc atggtgggct agctcacgac acctgaaatg gaagaaaaaa
      661 actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa
      721 ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt
      781 gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa
      841 gcgcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc
      901 gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc
      961 cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc
     1021 cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg
     1081 cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct
     1141 cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc
     1201 gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac
     1261 aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag
     1321 agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc
     1381 ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg
     1441 cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag
     1501 ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg
     1561 ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt
     1621 cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca
     1681 gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt
     1741 gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg
     1801 ggcacatgca tggcggtaat acggttatcc acgcggccgc ctaatggatc ctagtactcg
     1861 agaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttattta
     1921 aacttgctat gctgtttcca gcttagctct taaacgggtg gtgcccatcc tggtccaaca
     1981 aggtggttct ccaagggata cttatagtct caaaacacac aattacttta cagttagggt
     2041 gagtttcctt ttgtgctgtt tataacttcg tataatgtat gctatacgaa gttatagaaa
     2101 tccaagccta tcatgtaaaa tgtagctagt attaaaaaga acagattatc tgtcttttat
     2161 cgcacattaa gcctctatag ttactaggaa atattatatg caaattaacc ggggcagggg
     2221 agtagccgag cttctcccac aagtccgtgc gagggggccg gcgcgggcct agagatggcg
     2281 cgtcggatcg ttaacgtcga gggacctaat aacttcgtat agcatacatt atacgaagtt
     2341 atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct tatcgataat
     2401 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2461 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2521 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2581 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2641 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2701 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2761 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2821 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2881 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2941 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3001 cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     3061 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     3121 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     3181 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     3241 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     3301 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     3361 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     3421 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     3481 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     3541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3661 cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     5521 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5581 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     5641 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     5701 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     5761 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5821 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5881 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5941 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6001 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6061 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6121 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     6181 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     6241 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     6301 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     6361 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6421 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6481 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6541 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6601 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6661 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6721 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6781 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6841 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6901 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6961 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7021 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     7861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     7921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     7981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     8041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     8101 gtttggttag taccgggccc gctctagacc atagagccca ccgcatcccc agcatgcctg
     8161 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     8221 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     8281 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     8341 ctagaaggca cagtcgaggc tgatcagcgg gtttaaacgg gccctctagg ccctaggaat
     8401 tcttaattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc cactgccacc
     8461 tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag tctgtagtcc
     8521 acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct atatgtggtc
     8581 ttgatgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc gtttctgcct
     8641 tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc gagtgttttc
     8701 ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac gttgtagatg
     8761 aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat
     8821 gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa gtcggggatg
     8881 ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc caggatgtcg
     8941 aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg
     9001 tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac ggtgccctcc
     9061 atgtacagct tcatgtgcat gttctcctta atcagctcgc tggtggcgac cggtgggcca
     9121 ggattctcct cgacgtcacc gcatgttagc a
//
