LOCUS Exported 13119 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For gene editing in oomycetes. ACCESSION . VERSION . KEYWORDS pCB301TOR-CRISPR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13119) AUTHORS Gumtow R, Wu D, Uchida J, Tian M TITLE A Phytophthora palmivora Extracellular Cystatin-Like Protease Inhibitor Targets Papain to Contribute to Virulence on Papaya. JOURNAL Mol Plant Microbe Interact. 2018 Mar;31(3):363-373. doi: 10.1094/MPMI-06-17-0131-FI. Epub 2018 Jan 3. PUBMED 29068239 REFERENCE 2 (bases 1 to 13119) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13119 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(3830..3846) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3830..3846) /label=pBluescriptSK /note="For pBluescript vector" CDS 5007..5801 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5061..5080) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5671..5690 /label=Neo-F /note="Neomycin resistance gene, forward primer" misc_RNA 8184..8259 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 8628..8652 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" rep_origin 8732..9363 /label=oriV /note="incP origin of replication" CDS 9572..10366 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" CDS 10665..11813 /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" misc_feature 11968..11992 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 12614..12630 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 12615..12631 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 12781..12801 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 12826..3807 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" ORIGIN 1 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc 61 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga 121 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc 181 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc 241 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac 301 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc 361 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga 421 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc 481 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg 541 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc 601 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc 661 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg 721 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac 781 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc 841 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg 901 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg 961 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag 1021 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac 1081 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag 1141 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat 1201 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc 1261 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc 1321 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg 1381 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa 1441 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa 1501 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc 1561 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat 1621 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg 1681 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc 1741 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac 1801 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc 1861 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag 1921 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc 1981 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag 2041 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac 2101 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg 2161 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac 2221 catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga 2281 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg 2341 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat 2401 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga 2461 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg 2521 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag 2581 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac 2641 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa 2701 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg 2761 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac 2821 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag 2881 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat 2941 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag 3001 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg 3061 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg 3121 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt 3181 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc 3241 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct 3301 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa 3361 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc 3421 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg 3481 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga 3541 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat 3601 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga 3661 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc 3721 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg 3781 atcgacctgt ctcagctggg aggcgactaa gaattcctgc agcccggggg atccactagt 3841 tctagagcgg ccgccaccgc ggggtgccta cttcttgtaa gccgaaggtc gagctgtgtg 3901 cactgagtgc gaagttgcag attttgtgtc tatgcattta atacaatttc cttttttcca 3961 ttttcgttgt tttttttttg aaagaacgat tgttttcaaa agaaggccgt ttgtagaaca 4021 attttttttt atactagcat ttggtattcg agtgtgacga atcggtgatg gcgctttaca 4081 aatgaaatat aacgctgaac aagcgcagtg acagctttat tttataacag agaataaaga 4141 ccaaagtaga gtagactgat ggcggcttga aacaaaacta cgtgacttct aatgcaaaga 4201 aaaccatacc ccacgctact tagaattgtt tctctttggt tgatgaccgc tatagtcgta 4261 tggagtgatc cctttttgtc aatatttcga ttcttgctaa aaccgtgact attttgaggc 4321 aactgtgtgt acttaacatg gtagaaattt ctatgtttaa gccagctgac aatcattctt 4381 tatttatcgt aaaccaacca ccatatgctc aatgcaattg actgtgtcct cgattatttc 4441 tattatttaa tattatttcc attaaaaaag aaatccagtt cgtggcaaaa taagacaaag 4501 gtttaaataa aaatttatcg ttattaaact cgtaacttac cttcttcatg gctcaaccca 4561 atctgtatat cgacttattt aatttggtta acaaatcggt tttcgtcgca aataggggat 4621 gacaactaaa ataccaattt gtgtccttgc gacacgtcaa caggtggctg ccaatatctc 4681 aggttttgag cacacaacgc tgtgattggc tatctgtaga tcgcttcgag aactttcgga 4741 ccgacatatc agaacgcagt catgtccaat ccagctaacg attggtcgaa accgcacttc 4801 atcgcttcat ttcaaccaat tcccatctac taaaacgagc gtcggaatag tctcgaacgt 4861 aaccccacgt agtgggtggc agcgcccctc gtatctttca atcgccactc gagtctcaag 4921 tttgctcact ttgaaatttt ccatctggac tacacatcaa atctaactca acttttatca 4981 attgaaacac aagggccccg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 5041 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 5101 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 5161 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 5221 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 5281 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 5341 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 5401 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 5461 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 5521 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 5581 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 5641 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 5701 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 5761 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 5821 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 5881 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5941 cggggatctc atgctggagt tcttcgccca ccccccggat ccccggggtc gactagaata 6001 cttagcaatt ggttagtttg aaaattgtca aagcagagtt ttttcgacaa taaaatttat 6061 tttctaaata tttgatttag cgttgactta atttgttgat tcggttgtca tcttttgtga 6121 agatgcccga gagtctagta aatctcaagc gaaacatagt gagaaggtca ctatggctgg 6181 tttgcatgag tctgggccta ttgtgcttgg taccgttccg tcatttcctc gcagcaactc 6241 gcttccacga tttctttgta ttcactcggc agcctcgagc tgattgagcg agagatggac 6301 gtccacgccg atggatcacc cgtttggccg caatatttgc ctggactgtt agatcccgta 6361 ccagagatag acatggtcag cgacggcttc attacctcgt cggaatcatt gctggaacat 6421 caaacaagtt ttatctacgg cttcgataac cctgagtcga cggcatcatc tggagcattg 6481 gtagatgctg atgacgtgtg ctcttccacc acttctagac tgtgtatcaa gctccggttc 6541 acaggcgccg atcagttggc tccaatattt cttcgtaaac tgcagagcat tcacgctgca 6601 ctatcttcgt caattggtct tcctcccgga tcaagtgttg ctggttcaac ttctacttat 6661 ccggctccgt gggtgctggt tggtgctcag agtctcggtg gggatacttc gtggtccttc 6721 ttcgtaggcg ggtcgtcatc aagcggcaat gcccagagct tccgtctcac gttcgaagct 6781 gtaaactcga tgcaagatat gaccactacc acacccttca ccatagacct ggagtttgtt 6841 cctgacgacc caactgtcat ccccattgtg cgacgtcggc agtttggcga cctcgcggtg 6901 gtagacacca ctggccagcc tgaaccagca tttatcacct ctgagattcc tgatctgatc 6961 gacgatggag acgaagtcac tggtatcacc ataacattgg atttggagca gcctcgactt 7021 ggtggactgt attcaggttc acaactggat gatgtagccc caacttgcag cgagtgcacg 7081 acgttattgg aggattgcag cagctcgccc gagtgccgag cgttctcagc gtgtatgggt 7141 gttctgcttg aggcagactt gacactgata gctacgatgc tggagaagga cgatatcgac 7201 agcagcgttg atgcaacttg gctgctgcag gactgcttga gtccatcgga cggcacggaa 7261 tggtcgagta cagtgcgtga gctgctcgag tccagctacg cttgtctgtg ggcgaagagc 7321 tgtccactgg catacagcac cacaataggg aggcagatcg tgctggacta cagtcctgga 7381 gagcagatct tgaccttcga cctggcgagc ggattcgcca gcgtcagctt cgagctcggc 7441 aactgggcgt acagttttgt cgaggacttc acggcgaacg cgacggaagt gacagcccaa 7501 ctggacacga tgctgatgga gatgtaccgc actgcgctgt cgaatgtggc gacggtgaag 7561 tcggccctcg tcacgacgga agactcgaac acgggagtgg tgacggcgca gttgaccatt 7621 ctctacgagt tcctgggcgc actgccacag ccttatgtcg cagagaatgg aggttcgttg 7681 gcgtgcaata ccaccgagga ggctctgcat ctgcgcgtac tagccataac gtaagaacga 7741 gtgcaataac aagaacaact tcacttgcag tgcaagttga agacactata gtggggaaag 7801 tgtcactcaa gtggggtcac attttggttg gccagtagtc cgcgcccgct gtccagccaa 7861 tcaaagtgtt tgaagattcc atctcctctt ttttgcacca tacaacgccc ttcatgcggg 7921 acttgttgcc tacaataacc aagtctctcc acgctggcaa gctcgcagcc aactatcctc 7981 tcgcatgttg atcccaaggc tgcgctcgcc gacagcaaca agcctcactt tctgctgact 8041 gctctgcttg gaacatcgtt cggggtaagt ggcgaccccc ttgtctctct ctgagcctcg 8101 cagctattgg ccccacatgg ctacatcgac agtctttact tactatagtt ttaattcgtt 8161 cggtgacgct agctagggtc tcggttttag agctagaaat agcaagttaa aataaggcta 8221 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttggccggc atggtcccag 8281 cctcctcgct ggcgccggct gggcaacatg cttcggcatg gcgaatggga caccggtgtc 8341 gactagaata cttagcaatt ggttagtttg aaaattgtca aagcagagtt ttttcgacaa 8401 taaaatttat tttctaaata tttgatttag cgttgactta atttgttgat tcggttgtca 8461 tcttttgtga agatgcccga gagtctagta aatctcaagc gaaacatagt gagaaggtca 8521 ctatggctgg tttgcatgag tctgggccta ttgtgcttgg taccaaaacc accccagtac 8581 tttaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa 8641 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca 8701 ctcgatacag gcagcccatc agtccactag agacgctcac cgggctggtt gccctcgccg 8761 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg 8821 cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca 8881 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg 8941 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa 9001 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc 9061 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac 9121 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca 9181 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg 9241 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc 9301 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc 9361 gctcgagttg cagcatcacc cataattgtg gtttcaaaat cggctccgtc gatactatgt 9421 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aagttttaga 9481 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta 9541 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt 9601 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa 9661 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta 9721 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa 9781 gctgcctgtt ccaaaggtcc tgcaccttga acggcatgat ggctggagca atctgctcat 9841 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa 9901 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg 9961 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga 10021 tctggccgat gtggattgcg aaaactggga agaagacacc ccatttaaag atccgcgcga 10081 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga 10141 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg 10201 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga 10261 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga 10321 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc 10381 gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct 10441 ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca 10501 agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg 10561 ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc 10621 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg 10681 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa 10741 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga 10801 tggcccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc 10861 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt 10921 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg 10981 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca 11041 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca 11101 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga 11161 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga 11221 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt 11281 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt 11341 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt 11401 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg 11461 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg 11521 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat 11581 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc 11641 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca 11701 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg 11761 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg 11821 tggggtcagt tccggctggg ggttcagcag ccagcgcttt actgagatct cctgtggttg 11881 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt 11941 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga 12001 tagtttaaac tgaaggcggg aaacgacaat ctgagctctc tgatggacaa agggtcgcct 12061 ctacgtaccc gcgacaatct cggcttttcc aatgtcgaca gctggcatat cgcacgcgag 12121 gttaggcgtc ttcagccggc gtaaccacaa acctcgagtc aagtaccctt tttcagacct 12181 taatttccgt gtccattccc tcgcgtttac acgaaggttt ctgagaaata ccacatgtca 12241 cagagagaga gaagattgga gaaccttctc aacctgaccc gtgcagagcg cttttgcgtc 12301 ctaccatccg ttaattatag caccaccacc acatgtctcg ccggaactct cttatccgct 12361 ctttttgatc gtcttttttg cgccccatta aagacatctc ttgcacaggg atcgtttctg 12421 attggccatt tcacttcctc ttttacatcc gaaaaccacc tgtttgtggg gttgtgaagc 12481 cacaaccgga cgctcaaaaa gcctcgcccg actcgcccac gatcggaagg ctcattctcc 12541 ttttcactct cacgtgccca agtcccaacc gactcttttt cgaccttcac tctcaccgac 12601 aacgggcccc ccctcgaggt cgacggtatc gatatgcaca agcgcaagcg cgaggacgac 12661 accaaggtcc gtcgtcgcat gcacaagcgc aagcgcgagg acgacaccaa ggtccgtcgt 12721 cgcatgcaca agcgcaagcg cgaggacgac accaaggtcc gtcgtcgcac tagtatggcc 12781 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc 12841 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag 12901 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac 12961 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga 13021 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc 13081 agcaacgaga tggccaaggt ggacgacagc ttcttccac //