LOCUS Exported 7330 bp ds-DNA circular SYN 15-DEC-2021 DEFINITION AAV packaging plasmid expressing Rep/Cap genes. ACCESSION . VERSION . KEYWORDS pAAV2/9n SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7330) TITLE AAV packaging plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 7330) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 15, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7330 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 17..39 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 31..48 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 32..48 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 58..77 /label=T7 /note="T7 promoter, forward primer" promoter 58..76 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 105..121 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 106..122 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 4111..4122 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" primer_bind complement(4574..4594) /label=T3 /note="T3 promoter, forward primer" promoter complement(4574..4592) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4613..4629) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4613..4629) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4626..4648) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4637..4653 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4661..4691) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4706..4727 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4844..4861) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5015..5603) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5095..5114) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5774..6634) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6397..6416 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6635..6739) /gene="bla" /label=AmpR promoter rep_origin complement(6765..7220) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6902..6923) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7114..7133 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa 61 tacgactcac tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat 121 cgggggagct cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc 181 cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca 241 tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg 301 acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg 361 actttctgac ggaatggcgc cgtgtgagta aggccccgga ggctcttttc tttgtgcaat 421 ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat 481 ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc 541 gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg 601 gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc 661 agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc 721 tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc 781 agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag 841 ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt 901 ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc 961 aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg 1021 actacctggt gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt 1081 tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga 1141 aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca 1201 acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg 1261 agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga 1321 tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg 1381 accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca 1441 ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc 1501 aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca 1561 ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc 1621 atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata 1681 taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag 1741 cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc 1801 tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat atctgcttca 1861 ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg 1921 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga aaggtgccag 1981 acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc tttgaacaat 2041 aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac 2101 cttagtgaag gaattcgcga gtggtgggct ttgaaacctg gagcccctca acccaaggca 2161 aatcaacaac atcaagacaa cgctcgaggt cttgtgcttc cgggttacaa ataccttgga 2221 cccggcaacg gactcgacaa gggggagccg gtcaacgcag cagacgcggc ggccctcgag 2281 cacgacaagg cctacgacca gcagctcaag gccggagaca acccgtacct caagtacaac 2341 cacgccgacg ccgagttcca ggagcggctc aaagaagata cgtcttttgg gggcaacctc 2401 gggcgagcag tcttccaggc caaaaagagg cttcttgaac ctcttggtct ggttgaggaa 2461 gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca ggaaccggac 2521 tcctccgcgg gtattggcaa atcgggtgca cagcccgcta aaaagagact caatttcggt 2581 cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc tcccgcagcc 2641 ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt ggcagacaat 2701 aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga ttcccaatgg 2761 ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac ctacaacaat 2821 cacctctaca agcaaatctc caacagcaca tctggaggat cttcaaatga caacgcctac 2881 ttcggctaca gcaccccctg ggggtatttt gacttcaaca gattccactg ccacttctca 2941 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcctaa gcgactcaac 3001 ttcaagctct tcaacattca ggtcaaagag gttacggaca acaatggagt caagaccatc 3061 gccaataacc ttaccagcac ggtccaggtc ttcacggact cagactatca gctcccgtac 3121 gtgctcgggt cggctcacga gggctgcctc ccgccgttcc cagcggacgt tttcatgatt 3181 cctcagtacg ggtatctgac gcttaatgat ggaagccagg ccgtgggtcg ttcgtccttt 3241 tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg gtaacaactt ccagttcagc 3301 tacgagtttg agaacgtacc tttccatagc agctacgctc acagccaaag cctggaccga 3361 ctaatgaatc cactcatcga ccaatacttg tactatctct caaagactat taacggttct 3421 ggacagaatc aacaaacgct aaaattcagt gtggccggac ccagcaacat ggctgtccag 3481 ggaagaaact acatacctgg acccagctac cgacaacaac gtgtctcaac cactgtgact 3541 caaaacaaca acagcgaatt tgcttggcct ggagcttctt cttgggctct caatggacgt 3601 aatagcttga tgaatcctgg acctgctatg gccagccaca aagaaggaga ggaccgtttc 3661 tttcctttgt ctggatcttt aatttttggc aaacaaggaa ctggaagaga caacgtggat 3721 gcggacaaag tcatgataac caacgaagaa gaaattaaaa ctactaaccc ggtagcaacg 3781 gagtcctatg gacaagtggc cacaaaccac cagagtgccc aagcacaggc gcagaccggc 3841 tgggttcaaa accaaggaat acttccgggt atggtttggc aggacagaga tgtgtacctg 3901 caaggaccca tttgggccaa aattcctcac acggacggca actttcaccc ttctccgctg 3961 atgggagggt ttggaatgaa gcacccgcct cctcagatcc tcatcaaaaa cacacctgta 4021 cctgcggatc ctccaacggc cttcaacaag gacaagctga actctttcat cacccagtat 4081 tctactggcc aagtcagcgt ggagatcgag tgggagctgc agaaggaaaa cagcaagcgc 4141 tggaacccgg agatccagta cacttccaac tattacaagt ctaataatgt tgaatttgct 4201 gttaatactg aaggtgtata tagtgaaccc cgccccattg gcaccagata cctgactcgt 4261 aatctgtaat tgcttgttaa tcaataaacc gtttaattcg tttcagttga actttggtct 4321 ctgcgaaggg cgaattcgtt taaacctgca ggactagagg tcctgtatta gaggtcacgt 4381 gagtgttttg cgacattttg cgacaccatg tggtcacgct gggtatttaa gcccgagtga 4441 gcacgcaggg tctccatttt gaagcgggag gtttgaacgc gcagccgcca agccgaattc 4501 tgcagatatc catcacactg gcggccgctc gactagagcg gccgccaccg cggtggagct 4561 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc 4621 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 4681 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 4741 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 4861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 4921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 5101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 5161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 5341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 5401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 5461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 5521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 5581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 5641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 5701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 5761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 5821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 5881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 5941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 6001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 6061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 6121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 6181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 6241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 6301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 6361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 6421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 6481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 6541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 6601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 6661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 6721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa 6781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 6841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 6901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 6961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 7021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 7081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 7141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 7201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg 7261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 7321 attaagttgg //