LOCUS       Exported                2915 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  TERRA with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR TERRA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K
  TITLE     Telomere Transcripts Target Telomerase in Human Cancer Cells.
  JOURNAL   Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 
            10.3390/genes7080046.
  PUBMED    27537914
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(214..313)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(330..349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(331..349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(354..370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(354..370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(472..491)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             483..1292
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(550..569)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1439..2027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1928..1947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2181..2198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2357..2384
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2476..2562
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2611..2633
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2625..2642
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2626..2642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2658..2757
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
       61 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
      121 ttagggttag ggttagggtt agggttaggg ttcggaattc atcgatgata tcagatcgac
      181 aattccactt tttttaattc tcgagatatc tagacccagc tttcttgtac aaagttggca
      241 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      301 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
      361 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
      421 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
      481 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
      541 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
      601 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
      661 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
      721 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
      781 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
      841 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
      901 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
      961 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1021 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1081 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     1141 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     1201 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     1261 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     1321 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     1381 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1561 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2281 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     2341 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     2401 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     2461 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     2521 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     2581 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     2641 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
     2701 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttta
     2761 aaggaaccaa ttcagtcgat cgactggacg aattccgcgg aattccggtt ggggttgggg
     2821 ttggggttgg ggttggggtt agggttgggg ttagggttag cgttagggtt agggttaggg
     2881 ttggggttag ggttagggtt agggttaggg ttagg
//
