LOCUS Exported 13380 bp ds-DNA circular SYN 24-AUG-2022 DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.. ACCESSION . VERSION . KEYWORDS pCMV-dR8.2 dvpr SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13380) AUTHORS Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD TITLE Lentivirus-delivered stable gene silencing by RNAi in primary cells. JOURNAL RNA 2003 Apr;9(4):493-501. PUBMED 12649500 REFERENCE 2 (bases 1 to 13380) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 24, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13380 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" CDS 855..2357 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS 2150..5161 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 4846..4963 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 6458..6691 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 6876..6920 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" CDS 7214..7240 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" promoter complement(8477..8495) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8478..8495) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(8785..8804) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(9018..9037) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9137..9159 /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter 9283..9387 /gene="bla" /label=AmpR promoter CDS 9388..10248 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9606..9625) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10419..11007 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10908..10927 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11161..11178 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(11248..11268) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 11253..11582 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11433..11568 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11495..11514 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 12716..12781 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 12911..12931 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg 721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgcg acggatcgat 781 cccgtaataa gcttcgaggt ccgcggccgc gttgacgcgc acggcaagag gcgaggggcg 841 gcgactggtg agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgat 901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 1021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac 1201 agcaagcagc agctgacaca ggacacagca atcaggtcag ccaaaattac cctatagtgc 1261 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg 1321 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat 1381 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag 1441 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc 1501 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca 1561 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta 1621 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa 1681 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact 1741 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt 1801 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag 1861 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac 1921 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca 1981 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg 2041 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat 2101 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga 2161 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa 2221 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc 2281 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg 2341 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc 2401 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg 2461 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg 2521 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa 2581 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc 2641 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga 2701 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa 2761 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac 2821 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga 2881 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact 2941 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc 3001 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct 3061 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga 3121 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt 3181 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct 3241 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg 3301 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa 3361 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca 3421 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact 3481 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat 3541 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat 3601 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct 3661 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac 3721 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt 3781 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac 3841 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt 3901 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga 3961 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct 4021 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc 4081 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca 4141 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga 4201 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga 4261 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca 4321 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc 4381 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc 4441 catgcatgga caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga 4501 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat 4561 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc 4621 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc 4681 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg 4741 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc 4801 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg 4861 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 4921 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 4981 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt 5041 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag 5101 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta 5161 acacatggaa aagattagta aaacaccata tgtatatttc aaggaaagct aaggactggt 5221 tttatagaca tcactatgaa agtactaatc caaaaataag ttcagaagta cacatcccac 5281 taggggatgc taaattagta ataacaacat attggggtct gcatacagga gaaagagact 5341 ggcatttggg tcagggagtc tccatagaat ggaggaaaaa gagatatagc acacaagtag 5401 accctgacct agcagaccaa ctaattcatc tgcactattt tgattgtttt tcagaatctg 5461 ctataagaaa taccatatta ggacgtatag ttagtcctag gtgtgaatat caagcaggac 5521 ataacaaggt aggatctcta cagtacttgg cactagcagc attaataaaa ccaaaacaga 5581 taaagccacc tttgcctagt gttaggaaac tgacagagga cagatggaac aagccccaga 5641 agaccaaggg ccacagaggg agccatacaa tgaatggaca ctagagcttt agtagcccga 5701 acccggggcg gaccgaagtg gaagccataa taagaattct gcaacaactg ctgtttatcc 5761 atttcagaat tgggtgtcga catagcagaa taggcgttac tcgacagagg agagcaagaa 5821 atggagccag tagatcctag actagagccc tggaagcatc caggaagtca gcctaaaact 5881 gcttgtacca attgctattg taaaaagtgt tgctttcatt gccaagtttg tttcatgaca 5941 aaagccttag gcatctccta tggcaggaag aagcggagac agcgacgaag agctcatcag 6001 aacagtcaga ctcatcaagc ttctctatca aagcagtaag tagtacatgt aatgcaacct 6061 ataatagtag caatagtagc attagtagta gcaataataa tagcaatagt tgtgtggtcc 6121 atagtaatca tagaatatag gaaaatatta agacaaagaa aaatagacag gttaattgat 6181 agactaatag aaagagcaga agacagtggc aatgagagtg aaggagaagt atcagcactt 6241 gtggagatgg gggtggaaat ggggcaccat gctccttggg atattgatga tctgtagttg 6301 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat 6361 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga 6421 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct 6481 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac 6541 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac 6601 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg 6661 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca 6721 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt 6781 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac 6841 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat 6901 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa 6961 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt 7021 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa 7081 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag 7141 acagatccat tcgattagtg aacggatcct tggcacttat ctgggacgat ctgcggagcc 7201 tgtgcctctt cagctaccac cgcttgagag acttactctt gattgtaacg aggattgtgg 7261 aacttctggg acgcaggggg tgggaagccc tcaaatattg gtggaatctc ctacaatatt 7321 ggagtcagga gctaaagaat agtgctgtta gcttgctcaa tgccacagcc atagcagtag 7381 ctgaggggac agatagggtt atagaagtag tacaaggagc ttgtagagct attcgccaca 7441 tacctagaag aataagacag ggcttggaaa ggattttgct ataagatggg tggcaagtgg 7501 tcaaaaagta gtgtgattgg atggcctact gtaagggaaa gaatgagacg agctgagcca 7561 gcagcagatg gggtgggagc agtatctcga gacctagaaa aacatggagc aatcacaagt 7621 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 7681 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 7741 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 7801 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag 7861 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta 7921 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta 7981 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt 8041 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggagta cttcaagaac 8101 tgctgagcgg ccgccccggt gaccttcaga ccttggcact ggaggtggcc cggcagaagc 8161 gcggcatcgt ggatcagtgc tgcaccagca tctgctctct ctaccaactg gagaactact 8221 gcaactaggc ccaccactac cctgtccacc cctctgcaat gaataaaacc tttgaaagag 8281 cactacaagt tgtgtgtaca tgcgtgcatg tgcatatgtg gtgcgggggg aacatgagtg 8341 gggctggctg gagtggcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg 8401 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagtctag 8461 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta 8521 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg 8581 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg 8641 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa 8701 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg 8761 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 8821 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 8881 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 8941 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 9001 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 9061 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 9121 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 9181 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 9241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 9301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 9361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 9421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 9481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 9541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 9601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 9661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 9721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 9781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 9841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 9901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 9961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 10021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 10081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 10141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 10201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 10261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 10321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 10381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 10441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 10501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 10561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 10621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 10681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 10741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 10801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 10861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 10921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 10981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 11041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 11101 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 11161 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 11221 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 11281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 11341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 11401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 11461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 11521 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 11581 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg 11641 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg 11701 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat 11761 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag 11821 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt 11881 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc 11941 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc 12001 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg 12061 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg 12121 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct 12181 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat 12241 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag 12301 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg 12361 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc 12421 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg 12481 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag 12541 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt 12601 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta 12661 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa 12721 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 12781 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa 12841 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 12901 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 12961 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta 13021 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac 13081 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag 13141 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat 13201 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca 13261 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 13321 acctgaaaca taaaatgaat gcaattgttg ttgttgggct gcaggaattc gagctcgccc //