LOCUS       Exported                9026 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pABCb10-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Graf SA, Haigh SE, Corson ED, Shirihai OS
  TITLE     Targeting, import, and dimerization of a mammalian mitochondrial ATP
            binding cassette (ABC) transporter, ABCB10 (ABC-me).
  JOURNAL   J Biol Chem. 2004 Oct 8. 279(41):42954-63.
  PUBMED    15215243
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3251..3268
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3322..3895
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3489..3506)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3716..3735
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3957..3966
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3963..4682
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3963..4679
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4008..4029)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4269..4288)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4616..4637
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4695..4712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    4730..4851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4741..4760)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4795..4814
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5037..5492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5124..5143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5334..5355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5609..5966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(5632..5652)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5817..5952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5879..5898
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6017..6811
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6071..6090)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6681..6700
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6875..6923
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6964..6983)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7083..7105
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7143..7161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7229..7333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7334..8194
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7552..7571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8365..8953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8854..8873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgaggcca tgggcatgcg cgccccttct gctagggcgc tactgctgat
     1141 tccgcgtcgg ggccctgccg tgcgagcgtg ggccccggcc gtctcctctc ggatatggct
     1201 ggcttctgaa tggaccccgc tcgtacgcgc gtggacctct ctgatccaca agccgggttc
     1261 gggcctccgc tttcccgcgc ccctatccgg gctgcctggc ggcgtggggc agtgggccac
     1321 ctcctcgggg gcccgcaggt gctgggtgct ggcaggaccc cgcgccgcac atcccctgtt
     1381 cgccaggctc cagggtgcag ctgccaccgg tgtgcgagac cttgggaacg actcgcagcg
     1441 gcgtcccgcg gcgaccgggc gctcagaagt atggaagctc ctagggctgg tgcgccccga
     1501 gcgcgggaga ctgtcagctg cagttgggtt tctggctgtg tccagtgtca tcaccatgtc
     1561 tgcccccttc ttcctgggaa ggatcattga tgtcatctat accaacccaa gcgagggcta
     1621 tggcgatagc ttgactcggc tctgtgctgt gctcacctgt gtgttcctgt gcggcgctgc
     1681 tgccaacggc attcgtgtct acctcatgca gtcttcaggt cagagcattg tgaacaggct
     1741 gcgcacctct ctgttctcct ccattctaag gcaagaggtt gctttctttg acaagacccg
     1801 cacaggcgaa ttaattaacc gcctctcctc ggataccgca ctcctggggc gctccgtgac
     1861 tgagaacctc tccgatggcc tgagagctgg ggcccaggcc tccgttggtg ttggcatgat
     1921 gttttttgtg tcaccaagtc tggccacctt tgttctgagt gtggtaccgc ccatctctgt
     1981 cctcgctgtg atttatgggc gatatctacg gaaactgtcc aaagccacac aggactcctt
     2041 ggcagaagcc acacagctag ccgaggagcg gattggaaac ataagaacca tccgagcttt
     2101 tgggaaggag atgacggaag tggagaagta caccggccga gtggaccagt tgctgcagct
     2161 ggcacagaag gaggccttgg cacgagccgg cttctttgga gcagctgggc tctcaggaaa
     2221 cctgattgtg ctgtctgtcc tgtacaaagg aggcctgctg atgggcagtg cccatatgac
     2281 agtgggcgag ctctcttcct tcctcatgta cgctttctgg gttggattga gcatcggagg
     2341 actgagttca ttctactccg agctgatgaa aggcctgggt gctggtgggc ggctctggga
     2401 gctgctggag agacagccac ggctgccttt taacgagggg atggtgttag atgagaaaac
     2461 cttccagggt gccctggagt tcagaaacgt gcacttcaca taccctgctc gcccagaggt
     2521 gtccgtattc caggatttca gtctttccat cccatctgga tctgtcacag cgctggttgg
     2581 cccaagtggt tctgggaaat caacagtggt ttcgctcctg ctgcggttgt acgaccctaa
     2641 ttctggaaca gtcagccttg atggccatga cattcgtcag ctaaaccccg tctggctgag
     2701 atccaagatt gggacagtga gtcaggagcc agtcctgttc tcctgctctg tcgctgagaa
     2761 cattgcctat ggagctgaca acctctcctc ggtcacagcc cagcaggtgg agagggccgc
     2821 agaggtggcc aacgccgctg agttcatccg aagcttccca cagggcttcg acactgtggt
     2881 cggggagaag ggcatcctcc tgtcaggtgg gcagaagcag aggattgcaa tagccagagc
     2941 cctgctgaag aaccccaaaa ttcttctcct tgatgaagca accagtgcac tggatgccga
     3001 aaatgagcac ctggtccagg aggcccttga ccggctgatg gaagggagaa ccgtgctgat
     3061 catagcccac cgcctgtcca ccattaagaa tgccaacttt gttgctgtcc ttgaccacgg
     3121 aaagatctgt gaacacggga cacatgaaga gctgctcctg aagcccaatg ggctttacag
     3181 aaaactgatg aacaagcaga gtttcctgtt atataatgga gcagagcagt tcctggaacc
     3241 tgcacgggcg caccatcacc atcaccattg aacgcgtcga gcatgcatct agggcggcca
     3301 attccgcccc tctccccccc ccccctctcc ctcccccgcc cctaacgtta ctggccgaag
     3361 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3421 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3481 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3541 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3601 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3661 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3721 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3781 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     3841 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
     3901 ccacaacccg ggatcctcta gagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3961 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4021 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4081 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4141 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4201 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4261 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4321 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4381 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4441 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4501 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4561 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4621 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4681 aaagcggccg cttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt
     4741 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4801 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4861 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     4921 taaaacctct acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg
     4981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
     5041 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5101 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5161 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5221 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5281 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5341 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5401 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5461 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     5521 ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct
     5581 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
     5641 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5761 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5821 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5881 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5941 tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact
     6001 taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6061 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6121 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6181 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6241 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6301 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6361 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6421 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6481 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6541 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6601 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6661 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6721 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6781 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6841 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca
     6901 tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atccgcgtat ggtgcactct
     6961 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     7021 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7081 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     7141 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7201 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7561 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9001 ttttgctcac atggctcgac agatct
//
