LOCUS Exported 846 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pGEM-3z/623 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 846) AUTHORS Lowary PT, Widom J TITLE New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. JOURNAL J Mol Biol. 1998 Feb 13. 276(1):19-42. PUBMED 9514715 REFERENCE 2 (bases 1 to 846) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..846 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(353..371) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(354..371) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(389..405) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(389..405) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(402..424) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 413..429 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(437..467) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 482..503 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(620..637) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 ctatgggcga attcgagctc ggtacccggg gatcctctag agtcgggatc ctaatgacca 61 aggggggtgg gctacccaaa gcccacgata ccggctcata actggcgcct tttcaaagtt 121 gtacctgacc gagcaggtgc ctacagatcc agacgagcgg aaatgcccag aattgcgtct 181 acagaccgct aagctcatct agagctccct agagccacca agggctgttc ccagaaattg 241 tcgtagaacg ccaatcccct tcgcacaatg taaacgaacg cgttcgttta gtccgggtgc 301 cagtcggata gtgttccgag ctcccgacct gcaggcatgc aagcttgagt attctatagt 361 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 841 gtcaga //