LOCUS       Exported                 835 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKSV7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Smith K, Youngman P
  TITLE     Use of a new integrational vector to investigate 
            compartment-specific expression of the Bacillus subtilis spoIIM 
            gene.
  JOURNAL   Biochimie.   . 74(7-8):705-11.
  PUBMED    1391050
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    22..78
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(82..99)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(82..98)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(91..113)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(307..326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     426..448
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(486..504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        572..676
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagctat gacatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac
       61 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      241 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      301 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      361 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      421 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      481 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      541 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      601 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      661 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      721 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      781 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaag
//
