LOCUS Exported 5343 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Ras GTPase, Excitation: 488, Emission: 507. ACCESSION . VERSION . KEYWORDS EGFP-Rab11a-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5343) AUTHORS Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, Lambright DG, Luna EJ TITLE The membrane-associated protein, supervillin, accelerates F-actin-dependent rapid integrin recycling and cell motility. JOURNAL Traffic. 2010 Jun;11(6):782-99. doi: 10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17. PUBMED 20331534 REFERENCE 2 (bases 1 to 5343) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5343 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 547..556 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 553..1269 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 553..1269 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(598..619) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(859..878) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1206..1227 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2071..2192 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2108..2127) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2162..2181 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2199..2654) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2336..2357) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2548..2567 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2681..2785 /gene="bla" /label=AmpR promoter promoter 2787..3144 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2810..2830) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2995..3130 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3057..3076 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3179..3973 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3233..3252) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3843..3862 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4161..4180) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4205..4252 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4581..5169 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5070..5089 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1261 ctgtacaagt ccggactcag atctcgagcc atgggcaccc gcgacgacga gtacgactac 1321 ctctttaaag ttgtccttat tggagattct ggtgttggaa agagtaatct cctgtctcga 1381 tttactcgaa atgagtttaa tctggaaagc aagagcacca ttggagtaga gtttgcaaca 1441 agaagcatcc aggttgatgg aaaaacaata aaggcacaga tatgggacac agcagggcaa 1501 gagcgatatc gagctataac atcagcatat tatcgtggag ctgtaggtgc cttattggtt 1561 tatgacattg ctaaacatct cacatatgaa aatgtagagc gatggctgaa agaactgaga 1621 gatcatgctg atagtaacat tgttatcatg cttgtgggca ataagagtga tctacgtcat 1681 ctcagggcag ttcctacaga tgaagcaaga gcttttgcag aaaagaatgg tttgtcattc 1741 attgaaactt cggccctaga ctctacaaat gtagaagctg cttttcagac aattttaaca 1801 gagatttacc gcattgtttc tcagaagcaa atgtcagaca gacgcgaaaa tgacatgtct 1861 ccaagcaaca atgtggttcc tattcatgtt ccaccaacca ctgaaaacaa gccaaaggtg 1921 cagtgctgtc agaacatcta aggatccacc ggatctagat aactgatcat aatcagccat 1981 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2041 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2101 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2161 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 2221 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 2281 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 2341 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 2401 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 2461 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 2521 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 2581 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 2641 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 2701 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 2761 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 2821 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 2881 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2941 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3061 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3121 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 3181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 3241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 3301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 3361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 3421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 3481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 3541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 3601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 3661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 3721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 3781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 3841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 3901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 3961 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4021 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4141 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 4201 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 4261 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 4321 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 4381 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 4441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 4741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 4861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 5281 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 5341 ccg //