LOCUS Exported 10065 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag. ACCESSION . VERSION . KEYWORDS pLEX_307 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10065) TITLE Root Lab pLEX and pLIX Gateway backbones JOURNAL Unpublished REFERENCE 2 (bases 1 to 10065) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10065 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1418 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1443..1473 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1527..2186 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1593..1612) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2528..2833 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2712..2731 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2874..2998) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3006..3047 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3098..3686 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3151..3171) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3569..3580) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3758..3991 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4069..4190 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4106..4125) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4160..4179 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4230..4365 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4385..4404) /label=T7 /note="T7 promoter, forward primer" promoter complement(4386..4404) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4414..4431) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4414..4430) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4423..4445) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4572..5027 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4659..4678) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4869..4890 /label=F1ori-F /note="F1 origin, forward primer" promoter 5053..5157 /gene="bla" /label=AmpR promoter CDS 5158..6018 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5376..5395) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6189..6777 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6678..6697 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6931..6948 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7065..7086 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7101..7131 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7139..7155 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7144..7166 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7163..7179 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7163..7179 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7198..7218 /label=T3 /note="T3 promoter, forward primer" promoter 7200..7218 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7246..7472 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7473..7653 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7700..7825 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8318..8551 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8736..8780 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9011..9221 /label=SV40 promoter /note="SV40 early promoter" promoter 9033..9229 /label=SV40 promoter /note="SV40 early promoter" rep_origin 9080..9215 /label=SV40 ori /note="SV40 origin of replication" CDS 9261..9863 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(9261..9280) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9757..9777 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9906..10023 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agctgaacga 1321 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact 1381 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag 1441 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga 1501 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg 1561 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta 1621 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata 1681 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg 1741 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt 1801 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg 1861 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg 1921 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga 1981 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca 2041 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc 2101 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact 2161 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac 2221 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc 2281 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga 2341 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc 2401 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg 2461 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc 2521 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt 2581 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag 2641 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga 2701 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga 2761 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt 2821 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg 2881 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta 2941 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg 3001 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta 3061 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat 3121 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 3181 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 3241 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 3301 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 3361 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 3421 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 3481 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 3541 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 3601 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 3661 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct 3721 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 3781 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 3841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 3901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 3961 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt 4021 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 4081 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 4141 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 4201 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 4261 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4321 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 4381 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 4441 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 4501 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 4561 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 4621 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 4681 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 4741 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4801 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4861 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4921 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4981 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 5041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 5101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 5161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 5221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 5281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 5341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 5401 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 5461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 5521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 5581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 5641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 5701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 5761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 5821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 5881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 5941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 6001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 6061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 6121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 6181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 6241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 6301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 6361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 6421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 6481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 6541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 6601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 6661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 6721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 6781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 6841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 6901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 6961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 7021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 7081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 7141 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 7201 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa 7261 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga 7321 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa 7381 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata 7441 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag 7501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 7561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 7621 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc 7681 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc 7741 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa 7801 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga 7861 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc 7921 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg 7981 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc 8041 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac 8101 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca 8161 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat 8221 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga 8281 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct 8341 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac 8401 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac 8461 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg 8521 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca 8581 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt 8641 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac 8701 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat 8761 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa 8821 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt 8881 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa 8941 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 9001 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt 9061 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 9121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 9181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag 9241 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 9301 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 9361 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 9421 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg 9481 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 9541 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 9601 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 9661 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 9721 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 9781 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 9841 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc 9901 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 9961 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat 10021 tttcgggttt attacaggga cagcagagat ccactttggc gccgg //