LOCUS       Exported                4126 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expression in E coli and perform ligand binding in solution.
ACCESSION   .
VERSION     .
KEYWORDS    CXCR4-SZ158a QTY
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Qing R, Tao F, Chatterjee P, Yang G, Han Q, Chung H, Ni J, Suter BP,
            Kubicek J, Maertens B, Schubert T, Blackburn C, Zhang S
  TITLE     Non-full-length Water-Soluble CXCR4(QTY) and CCR5(QTY) Chemokine 
            Receptors: Implication for Overlooked Truncated but Functional 
            Membrane Receptors.
  JOURNAL   iScience. 2020 Oct 28;23(12):101670. doi: 
            10.1016/j.isci.2020.101670. eCollection 2020 Dec 18.
  PUBMED    33376963
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(185..202)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             711..733
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(764..783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(765..783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1292..1483
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1477..1499)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1585..1724
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1640..1659
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1739..1756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1910..2498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1990..2009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2669..3529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3292..3311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3530..3634)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3660..4115)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3797..3818)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4009..4028
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gaggtcgaca agcttggatc
      181 cttagtggtg gtggtggtgg tgcgccggcg caacctggct ggtctcggtg ctgctgtgaa
      241 agctgctgct ttcgctctcg gtgctcacgc tgctgtggcc accacgtttg cccttgctca
      301 gaatcttcag gctgctacca cggctaacgc tggtcagcgc gtgttgcgcg ctggttttaa
      361 acttcgcgcc caggaacgca tattgggtcg ggttctggca gcagtggaag tacgccagcg
      421 cttcggtggt gctaatccat ttgtgcacgg tgttttcaaa ctcgcagccc tgcttaatga
      481 tctccagcag gatgaagcta tcggtgctgg taccggtata gtacggttgc cagcacgcat
      541 agtacgcttg ggtctgggtg gtggtgctat aggtggtcgg ctgaaagatt ttgttgaagt
      601 tcgcgttttc ctcacggaag cacggttcct tcatgctatc gtagtcgccg ctacccattt
      661 cctcggtata gttgtcgctg gtgtaaatgc tgataccctc catatgtata tctccttctt
      721 aaagttaaac aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta
      781 ttaatttcgc gggatcgaga tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
      841 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
      901 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
      961 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     1021 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     1081 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
     1141 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
     1201 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
     1261 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     1321 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     1381 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     1441 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     1561 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     1621 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattgcggt
     1681 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     1741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2341 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     3001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     3661 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     3721 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3781 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3841 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     3901 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3961 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     4021 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     4081 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//
