LOCUS Exported 9270 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pQCXIH-Myc-YAP-5SA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9270) AUTHORS Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J, Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL TITLE Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. JOURNAL Genes Dev. 2007 Nov 1. 21(21):2747-61. PUBMED 17974916 REFERENCE 2 (bases 1 to 9270) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9270 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 505..529 /label=LNCX /note="Human CMV promoter, forward primer" CDS 653..682 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" misc_feature 2195..2768 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2362..2379) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2589..2608 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2807..3826 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE" LTR 4373..4798 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(4901..4923) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5059..5079) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5064..5393 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5244..5379 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5306..5325 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 5415..5434 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(5515..5532) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5686..6274) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5766..5785) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6445..7305) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7068..7087 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7306..7410) /gene="bla" /label=AmpR promoter primer_bind 7478..7496 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 7596..7975 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 7976..8179 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 8180..8355 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 8418..8617 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 8818..9234 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 9164..9186 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 9202..9218 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg 541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg 601 ggaacggtgc attggaacgg acctgcagga attgatccgc ggccgcacca tggaacaaaa 661 actcatctca gaagaggatc tggcgggcaa ggatcccggg cagcagccgc cgcctcaacc 721 ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc 781 cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg 841 gcatcagatc gtgcacgtcc gcggggacgc ggagaccgac ctggaggcgc tcttcaacgc 901 cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct 961 gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccgctactga 1021 tgcaggcact gcaggagccc tgactccaca gcatgttcga gctcatgccg ctccagctgc 1081 tctgcagttg ggagctgttt ctcctgggac actgaccccc actggagtag tctctggccc 1141 agcagctaca cccacagctc agcatcttcg acaggctgct tttgagatac ctgatgatgt 1201 acctctgcca gcaggttggg agatggcaaa gacatcttct ggtcagagat acttcttaaa 1261 tcacatcgat cagacaacaa catggcagga ccccaggaag gccatgctgt cccagatgaa 1321 cgtcacagcc cccaccagtc caccagtgca gcagaatatg atgaactcgg cttcaggtcc 1381 tcttcctgat ggatgggaac aagccatgac tcaggatgga gaaatttact atataaacca 1441 taagaacaag accacctctt ggctagaccc aaggcttgac cctcgttttg ccatgaacca 1501 gagaatcagt cagagtgctc cagtgaaaca gccaccaccc ctggctcccc agagcccaca 1561 gggaggcgtc atgggtggca gcaactccaa ccagcagcaa cagatgcgac tgcagcaact 1621 gcagatggag aaggagaggc tgcggctgaa acagcaagaa ctgcttcggc aggagttagc 1681 cctgcgtagc cagttaccaa cactggagca ggatggtggg actcaaaatc cagtgtcttc 1741 tcccgggatg tctcaggaat tgagaacaat gacgaccaat agctcagatc ctttccttaa 1801 cagtggcacc tatcactctc gagatgaggc tacagacagt ggactaagca tgagcagcta 1861 cagtgtccct cgaaccccag atgacttcct gaacagtgtg gatgagatgg atacaggtga 1921 tactatcaac caaagcaccc tgccctcaca gcagaaccgt ttcccagact accttgaagc 1981 cattcctggg acaaatgtgg accttggaac actggaagga gatggaatga acatagaagg 2041 agaggagctg atgccaagtc tgcaggaagc tttgagttct gacatcctta atgacatgga 2101 gtctgttttg gctgccacca agctagataa agaaagcttt cttacatggt tatagttaat 2161 taacggatcc ggaattccgc cccccccccc cccccccctc tccctccccc ccccctaacg 2221 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 2281 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 2341 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 2401 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 2461 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 2521 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 2581 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 2641 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 2701 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 2761 gatgataagc ttgccacaac ccgtaccaaa gatggataga tccggaaagc ctgaactcac 2821 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca 2881 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt 2941 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt 3001 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct 3061 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga 3121 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct 3181 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg 3241 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga 3301 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga 3361 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga 3421 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata 3481 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg 3541 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct 3601 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc 3661 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac 3721 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga 3781 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc 3841 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa 3901 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg 3961 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt 4021 ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg 4081 agatcgccgc ccgtgcctgc cgattgggtg cagatctcta tcgagggctg cagcgctgca 4141 gaggccgagt gcagaactgc tccaaaggga cctcaaggct ttccgaggga cactaggctg 4201 actccatcga gccagtgtag agataagctt atcgattagt ccaatttgtt aaagacagga 4261 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt 4321 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag 4381 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa 4441 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga 4501 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 4561 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 4621 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 4681 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 4741 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 4801 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt 4861 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 4921 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 4981 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 5041 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 5101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 5161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 5221 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 5281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 5341 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta 5401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 5461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 5521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 5581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 5641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 5701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 5761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 5821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 5881 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 5941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6001 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 6061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 6121 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 6181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 6241 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 6301 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 6361 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 6421 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 6481 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 6541 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 6601 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 6661 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 6721 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 6781 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 6841 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 6901 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 6961 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7021 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 7081 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7141 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 7201 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 7261 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 7321 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 7381 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 7441 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 7501 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat 7561 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 7621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 7681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 7741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 7801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 7861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 7921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 7981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 8041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 8101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 8161 ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct 8221 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat 8281 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga 8341 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg 8401 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 8461 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg 8521 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc 8581 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg 8641 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg 8701 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc 8761 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc 8821 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc 8881 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa 8941 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc 9001 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac 9061 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac 9121 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt 9181 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt 9241 gaagatctgg gggatcgatc ctctagagtc //